alphafold 3 server Search Results


alphafold3 (af) server  (Schrodinger LLC)
90
Schrodinger LLC alphafold3 (af) server
Live-cell imaging of cells expressing Sid4-GFP and Sad1-mCherry in ppc89 + , ppc89-3 , or ppc89-4 cells. Cells were grown at 25°C and were shifted to 36°C for 3 h and images were acquired at both time points. Scale bar, 5 µm. a) Quantification of SPB fluorescence intensity from cells as in (b) and S2B. Sid4-GFP SPB fluorescence intensity was divided by Sad1-mCherry SPB fluorescence intensity. N ≥ 30 cells for each condition and strain from 3 independent experiments. From 3 independent experiments. Error bars represent mean ± SEM. P -values were obtained with a 1-way ANOVA with Tukey's post hoc test. **** P ≤ 0.0001. b) Representative images from the indicated strains. (c, e) Yeast 2-hybrid analysis was performed with S. cerevisiae strain PJ69-4A which was cotransformed with plasmids expressing the indicated Ppc89 and Sid4 fragments. Black boxes indicate regions of predicted α-helices. leu + trp + transformants were tested for growth on −his and -ade plates (SDMU) or -his plates (SDMUA). “+++” indicates strong growth, “++” indicates little growth, and “−” indicates no growth. d) <t>AlphaFold3</t> predicted structure of the Ppc89-Sid4 complex. Two copies of Ppc89s residues 647-687 were modeled with 2 copies of Sid4 residues 546-660. Ppc89 is in magenta and Sid4 is in gray. f) In vitro binding assay with bead-bound GST or GST-Ppc89(545-687) and soluble MBP or MBP-Sid4(546-660). Samples were washed, resolved by SDS-PAGE, and stained with Coomassie blue.
Alphafold3 (Af) Server, supplied by Schrodinger LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alphafold+3+server/pmc11708228-78-7-26?v=Schrodinger+LLC
Average 90 stars, based on 1 article reviews
alphafold3 (af) server - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


Live-cell imaging of cells expressing Sid4-GFP and Sad1-mCherry in ppc89 + , ppc89-3 , or ppc89-4 cells. Cells were grown at 25°C and were shifted to 36°C for 3 h and images were acquired at both time points. Scale bar, 5 µm. a) Quantification of SPB fluorescence intensity from cells as in (b) and S2B. Sid4-GFP SPB fluorescence intensity was divided by Sad1-mCherry SPB fluorescence intensity. N ≥ 30 cells for each condition and strain from 3 independent experiments. From 3 independent experiments. Error bars represent mean ± SEM. P -values were obtained with a 1-way ANOVA with Tukey's post hoc test. **** P ≤ 0.0001. b) Representative images from the indicated strains. (c, e) Yeast 2-hybrid analysis was performed with S. cerevisiae strain PJ69-4A which was cotransformed with plasmids expressing the indicated Ppc89 and Sid4 fragments. Black boxes indicate regions of predicted α-helices. leu + trp + transformants were tested for growth on −his and -ade plates (SDMU) or -his plates (SDMUA). “+++” indicates strong growth, “++” indicates little growth, and “−” indicates no growth. d) AlphaFold3 predicted structure of the Ppc89-Sid4 complex. Two copies of Ppc89s residues 647-687 were modeled with 2 copies of Sid4 residues 546-660. Ppc89 is in magenta and Sid4 is in gray. f) In vitro binding assay with bead-bound GST or GST-Ppc89(545-687) and soluble MBP or MBP-Sid4(546-660). Samples were washed, resolved by SDS-PAGE, and stained with Coomassie blue.

Journal: G3: Genes | Genomes | Genetics

Article Title: New mutations in the core Schizosaccharomyces pombe spindle pole body scaffold Ppc89 reveal separable functions in regulating cell division

doi: 10.1093/g3journal/jkae249

Figure Lengend Snippet: Live-cell imaging of cells expressing Sid4-GFP and Sad1-mCherry in ppc89 + , ppc89-3 , or ppc89-4 cells. Cells were grown at 25°C and were shifted to 36°C for 3 h and images were acquired at both time points. Scale bar, 5 µm. a) Quantification of SPB fluorescence intensity from cells as in (b) and S2B. Sid4-GFP SPB fluorescence intensity was divided by Sad1-mCherry SPB fluorescence intensity. N ≥ 30 cells for each condition and strain from 3 independent experiments. From 3 independent experiments. Error bars represent mean ± SEM. P -values were obtained with a 1-way ANOVA with Tukey's post hoc test. **** P ≤ 0.0001. b) Representative images from the indicated strains. (c, e) Yeast 2-hybrid analysis was performed with S. cerevisiae strain PJ69-4A which was cotransformed with plasmids expressing the indicated Ppc89 and Sid4 fragments. Black boxes indicate regions of predicted α-helices. leu + trp + transformants were tested for growth on −his and -ade plates (SDMU) or -his plates (SDMUA). “+++” indicates strong growth, “++” indicates little growth, and “−” indicates no growth. d) AlphaFold3 predicted structure of the Ppc89-Sid4 complex. Two copies of Ppc89s residues 647-687 were modeled with 2 copies of Sid4 residues 546-660. Ppc89 is in magenta and Sid4 is in gray. f) In vitro binding assay with bead-bound GST or GST-Ppc89(545-687) and soluble MBP or MBP-Sid4(546-660). Samples were washed, resolved by SDS-PAGE, and stained with Coomassie blue.

Article Snippet: Protein structure predictions were generated with the AlphaFold3 (AF) server ( Abramson et al. 2024 ) and visualized using the PyMOL molecular graphics system version 3.0 Schrodinger, LLC.

Techniques: Live Cell Imaging, Expressing, Fluorescence, In Vitro, Binding Assay, SDS Page, Staining