Journal: International Journal of Molecular Sciences

Article Title: Time-Dependent Changes in Muscle IGF1-IGFBP5-PAPP System after Sciatic Denervation

doi: 10.3390/ijms241814112

Figure Lengend Snippet: Effect of sciatic denervation on the IGFBP-5/PAPP-A expression levels ratio ( A ), total protein concentration ( B ) and the protein levels of HDAC-4 ( C ), myogenin ( D ), cleaved alpha II spectrin ( E ) and light chain 3b II (LC3b-II) ( F ) at different time points post-denervation (DNX). Values are represented as mean ± standard error of the mean (SEM) of right (denervated) paws in comparison with control animals (SHAM operated in both hind limbs). Representative Western blots are shown in ( G ). Statistics: a ≠ b ≠ c in the comparisons between different time-point groups of the right paw by least post hoc analysis after significant one-way ANOVA.

Article Snippet: After blocking the membranes with TBS containing 5% ( w / v ) non-fat dried milk, they were incubated overnight at 4 °C with primary antibodies: HDAC4 (antibody ID: 7628, 1:2000; Cell Signaling Technology; Danvers, MA, USA); Myogenin (antibody ID: sc-12732, 1:500; Santa Cruz Biotechnology; Dallas, TX, USA); LC3b (antibody ID: 12741, 1:1000; Cell Signaling Technology; Danvers, MA, USA) and alpha 2 spectrin (antibody ID: sc-48382; 1:1000; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Expressing, Protein Concentration, Comparison, Control, Western Blot

Journal: Archives of biochemistry and biophysics

Article Title: Endoplasmic Reticulum Stress-induced Complex I Defect: Central Role of Calcium Overload

doi: 10.1016/j.abb.2020.108299

Figure Lengend Snippet: Antibodies used in the current manuscript.

Article Snippet: Background intensity adjustment, if performed, was always adjusted for the entire membrane. table ft1 table-wrap mode="anchored" t5 Table 1: caption a7 Antibody name Company Catalog number Concentration Subunit 4 of cytochrome oxidase Cell signaling 4844 1:10000 GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) Cell signaling 5174 1:1000 NDUFA4 (mitochondrial complex associated) Abcam ab129752 1:1000 NDUFB1 (NADH:Ubiquinone Oxidoreductase Subunit B1) Abcam ab201302 1:1000 NDUFS7 (NADH:Ubiquinone Oxidoreductase Core Subunit S7) ThermoFisher Scientific PA5–19343 1:500 PDHα1 subunit (pyruvate dehydrogenase α1 subunit) Cell signaling 2784 1:1000 Spectrin Santa Cruz csc-46696 1:100 VDAC (Voltage-dependent anion-selective channel) Abcam ab14715 1:2500 Open in a separate window Antibodies used in the current manuscript.

Techniques: Concentration Assay