Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Extracellular Vesicle Marker Changes Associated With Disease Activity in Relapsing-Remitting Multiple Sclerosis

doi: 10.1212/NXI.0000000000200404

Figure Lengend Snippet: EVs were isolated by size-exclusion chromatography (SEC, SmartSEC, System Biosciences). (A) Analysis of the classical EV marker Alix by Western blotting after isolation. Equal amounts of EVs and plasma samples (10 µg) were used. A weak level of albumin was detected in the EV fraction. (B) Freshly isolated EVs were subjected to cryo-electron microscopy (cryo-EM). Cryo-electron microscopy observations showed EVs with typical lipid bilayer membrane. EV = extracellular vesicle.

Article Snippet: Thereafter, membranes were incubated with a primary antibody against Alix (1:500, NBP1-90201, Novus Biologicals, Centennial, CO) in a rolling shaker overnight at 4°C.

Techniques: Isolation, Size-exclusion Chromatography, Marker, Western Blot, Clinical Proteomics, Cryo-Electron Microscopy, Cryo-EM Sample Prep, Membrane

Journal: Aging

Article Title: Hypoxia-regulated exosomes mediate M2 macrophage polarization and promote metastasis in chondrosarcoma.

doi: 10.18632/aging.205230

Figure Lengend Snippet: Figure 1. Isolation of exosomes derived from chondrosarcoma cells. (A) Identical concentrations of SW1353 cells were seeded under normoxic (O2 = 20%) and hypoxic (O2 = 1%) conditions for 48 h. Exosome (SW1353-N-exo and SW1353-H-exo) size was the characterization of exosomes by Nanoparticle Tracking Analysis (NTA). (B, C) Western blot analysis for exosomal proteins CD9, ALIX, TSG101, CD63, HSP70. All replicate number ≧ 3. *p < 0.05 compared with the SW1353-N-exo group.

Article Snippet: We obtained specific rabbit polyclonal antibodies for ALIX (Catalog No: A2215), TSG101 (Catalog No: A1692), and CD63 (Catalog No: A5274) from ABclonal (Woburn, MA, USA).

Techniques: Isolation, Derivative Assay, Western Blot