alignx application module Search Results


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bionumerics v. 4.61 sequence types module  (Applied Maths)
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alignx application module vector nti 7 software package  (InforMax Inc)
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dnastar software meg align module  (DNASTAR)
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align 2d structure alignment program  (Accelrys)
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alpha300  (Oxford Instruments)
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jsm-it800 scanning electron microscope  (JEOL)
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alpha actin antibody / acta2  (NSJ Bioreagents)
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Image Search Results


KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Modulation of Peptidoglycan Synthesis by Recycled Cell Wall Tetrapeptides

doi: 10.1016/j.celrep.2020.107578

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Penta-His antibody QIAGEN Cat#34660; RRID:AB_2619735 Anti-Mouse IgG Sigma Cat#A9044; RRID:AB_258431 Bacterial and Virus Strains All bacterial strains are listed in Table S1 This study Table S1 Chemicals, Peptides, and Recombinant Proteins D-Methionine Sigma Cat#M9375 L-Methionine Merck Cat#105707 Critical Commercial Assays Ni-NTA Agarose QIAGEN Cat#30210 Bio-Rad Protein Assay Dye Reagent Concentrate Bio-Rad Cat#500-0006 GeneJET Genomic DNA Purification Kit Thermo Fisher Scientific Cat#K0721 Qubit® dsDNA HS Assay Kit Thermo Fisher Scientific Cat# {"type":"entrez-protein","attrs":{"text":"Q32851","term_id":"75280859","term_text":"Q32851"}} Q32851 Nextera XT DNA Library Preparation Kit Illumina Cat#FC-131-1024 MiSeq Reagent Kit v2 (50-cycles) Illumina Cat#MS-102-2001 Deposited Data Tn-seq experiments_Raw data This study https://www.ncbi.nlm.nih.gov BioProject ID: PRJNA623082 WGS suppressor mutants_Raw data This study https://www.ncbi.nlm.nih.gov BioProject ID: PRJNA623074 Oligonucleotides All oligonucleotides are detailed in Tables S2 – S4 This study Tables S2 – S4 Recombinant DNA pET28b:: vc2153 -6xHis This study FC281 pET28b:: ldcA -6xHis This study FC2025 pET22b:: vca0337 -6xHis This study FC2662 pET28b:: slt70 -6xHis ( E. coli ) Espaillat et al., 2016 FC1860 pET28b:: ldtA -6xHis Cava et al., 2011 FC1168 pET28b::AHA1477-6xHis This study FC2772 pET28b::PMI1557-6xHis This study FC2773 pET28b::STM1800-6xHis This study FC2774 pBAD 33 :: ldcA- 6xHis This study FC2753 pBAD 18-Km :: ldtA- 6xHis This study FC2759 pBAD 18-Km :: murA- 6xHis This study FC2838 pBAD 18-Km :: murB- 6xHis This study FC2839 pBAD 18-Km :: murC- 6xHis This study FC2840 pBAD 18-Km :: murE- 6xHis This study FC2841 pBAD 18-Km :: murF- 6xHis This study FC2842 Software and Algorithms GraphPad Prism GraphPad Software, Inc. https://www.graphpad.com Zeiss Zen Blue Software Zeiss https://www.zeiss.com/corporate/int/home.html ImageJ https://imagej.nih.gov/ij/ https://imagej.nih.gov/ij/ NCBI blast https://www.ncbi.nlm.nih.gov/BLAST/ https://www.ncbi.nlm.nih.gov/BLAST/ Galaxy server tools Afgan et al., 2016 https://usegalaxy.org/ Open in a separate window KEY RESOURCES TABLE Vibrio cholerae releases peptidoglycan (PG) fragments edited with NCDAAs Recycling of NCDAA-muropeptides induces the accumulation of PG-tetrapeptide precursors V. cholerae recycled tetrapeptides are substrates for PG synthesis Recycled PG-tetrapeptides control cell wall synthesis and crosslinking

Techniques: Recombinant, DNA Purification, Software