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Key Resources Table

Journal: Structure (London, England : 1993)

Article Title: Structural basis for MARK1 kinase autoinhibition by its KA1 domain

doi: 10.1016/j.str.2018.05.008

Figure Lengend Snippet: Key Resources Table

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Overexpress C41(DE3) Sigma Aldrich Cat#CMC0017 Chemicals, Peptides, and Recombinant Proteins Recombinant human MARK1 45-795, Δ382-681, T215E/K761S/R764S, N-terminal hexahistidine (Crystal construct) ( Emptage et al., 2017a ) N/A Recombinant human MARK1 45-371, TEV-cleavable N-terminal hexahistidine (kinase/UBAdomain) ( Emptage et al., 2017a ) N/A Recombinant human MARK1 683-795, N-terminal hexahistidine (KA1 domain) ( Moravcevic et al, 2010 ) N/A Tau-derived MARK1 substrate peptide Genscript Cat#RP20244 Recombinant TEV protease pRK793 N/A Deposited Data Crystal structure of autoinhibited MARK1 This study 6C9D Recombinant DNA pET21a Recombinant human MARK1 45-795, Δ382-681, T215E/K761S/R764S, N-terminal hexahistidine (Crystal construct) (Emptage et al., 2015a) N/A pET21a Recombinant human MARK1 45-371, TEV-cleavable N-terminal hexahistidine (kinase/UBA domain) (Emptage et al., 2015a) N/A pET21a Recombinant human MARK1 683-795, N-terminal hexahistidine (KA1 domain) ( Moravcevic et al, 2010 ) N/A pRK793 (TEV protease) Addgene Plasmid#8827 Software and Algorithms HKL2000 ( Otwinowski and Minor, 1997 ) www.hkl-xray.com Coot ( Emsley et al., 2010 ) www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot PHENIX ( Adams et al., 2010 ) www.phenix-online.org/documentation/index.html MOLPROBITY ( Chen et al., 2010 ) http://molprobity.biochem.duke.edu PyMol Schrödinger www.pymol.org APBS ( Baker et al., 2001 ) www.poissonboltzmann.org/apbs GraphPad Prism 5 GraphPad Software, Inc. www.graphpad.com/scientific-software/prism/ PISA ( Krissinel and Henrick, 2007 ) www.ebi.ac.uk/pdbe/pisa/ ESPript ( Robert et al., 2014 ) http://espript.ibcp.fr Other HisPur Ni-NTA Resin Thermo Scientific Cat#88223 HiTrap SP HP GE Healthcare Cat#17115101 Superdex 75 10/300 GL GE Healthcare Cat#17517104 Open in a separate window Key Resources Table Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Ronen Marmorstein ( ude.nnepu@romram ).

Techniques: Virus, Recombinant, Construct, Plasmid Preparation, Software