alexa488-conjugated goat anti-rabbit igg Search Results


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  • 99
    Thermo Fisher anti rabbit alexa488 conjugated
    Anti Rabbit Alexa488 Conjugated, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam alexa488 conjugated goat anti rabbit igg h l
    Alexa488 Conjugated Goat Anti Rabbit Igg H L, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa488 conjugated goat anti rabbit igg
    Fig. 4. Actin cytoskeleton regulates localization of CD44H and MT1-MMP. CHO-K1 cells were transiently transfected with expression plasmids for CD44H-F ( A ), MT1-F ( B ), MT1-F/dPEX ( C ) and CD44H/dCP + MT1-F ( D and E ). Transfected cells were seeded on fibronectin-coated glass coverslips for 1 h and treated with anti-FLAG antibody (A–C and E) or anti-CD44 antibody (D) for 30 min at 37°C. After excess antibody had been washed off with PBS, the cells were treated with CyD (1 µg/ml) for 30 min at 37°C. They were then fixed, and the localization of the bound antibody and actin cytoskeleton was visualized with Cy3-conjugated anti-mouse <t>IgG</t> (red) and <t>Alexa488-conjugated</t> phalloidin (A–C and E) or Alexa488-conjugated anti-rabbit IgG and Alexa594-conjugated phalloidin (D), respectively. Scale bar, 10 µm.
    Alexa488 Conjugated Goat Anti Rabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 360 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa488 conjugated goat anti rabbit igg/product/Thermo Fisher
    Average 89 stars, based on 360 article reviews
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    Jackson Immuno alexa488 conjugated goat anti rabbit igg
    Fig. 4. Actin cytoskeleton regulates localization of CD44H and MT1-MMP. CHO-K1 cells were transiently transfected with expression plasmids for CD44H-F ( A ), MT1-F ( B ), MT1-F/dPEX ( C ) and CD44H/dCP + MT1-F ( D and E ). Transfected cells were seeded on fibronectin-coated glass coverslips for 1 h and treated with anti-FLAG antibody (A–C and E) or anti-CD44 antibody (D) for 30 min at 37°C. After excess antibody had been washed off with PBS, the cells were treated with CyD (1 µg/ml) for 30 min at 37°C. They were then fixed, and the localization of the bound antibody and actin cytoskeleton was visualized with Cy3-conjugated anti-mouse <t>IgG</t> (red) and <t>Alexa488-conjugated</t> phalloidin (A–C and E) or Alexa488-conjugated anti-rabbit IgG and Alexa594-conjugated phalloidin (D), respectively. Scale bar, 10 µm.
    Alexa488 Conjugated Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa488 conjugated goat anti rabbit igg/product/Jackson Immuno
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    93
    Abcam goat anti rabbit igg conjugated to alexa488 fluorophore
    Fig. 4. Actin cytoskeleton regulates localization of CD44H and MT1-MMP. CHO-K1 cells were transiently transfected with expression plasmids for CD44H-F ( A ), MT1-F ( B ), MT1-F/dPEX ( C ) and CD44H/dCP + MT1-F ( D and E ). Transfected cells were seeded on fibronectin-coated glass coverslips for 1 h and treated with anti-FLAG antibody (A–C and E) or anti-CD44 antibody (D) for 30 min at 37°C. After excess antibody had been washed off with PBS, the cells were treated with CyD (1 µg/ml) for 30 min at 37°C. They were then fixed, and the localization of the bound antibody and actin cytoskeleton was visualized with Cy3-conjugated anti-mouse <t>IgG</t> (red) and <t>Alexa488-conjugated</t> phalloidin (A–C and E) or Alexa488-conjugated anti-rabbit IgG and Alexa594-conjugated phalloidin (D), respectively. Scale bar, 10 µm.
    Goat Anti Rabbit Igg Conjugated To Alexa488 Fluorophore, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg conjugated to alexa488 fluorophore/product/Abcam
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    85
    Cell Signaling Technology Inc goat anti rabbit igg alexa488 conjugated secondary
    Fig. 4. Actin cytoskeleton regulates localization of CD44H and MT1-MMP. CHO-K1 cells were transiently transfected with expression plasmids for CD44H-F ( A ), MT1-F ( B ), MT1-F/dPEX ( C ) and CD44H/dCP + MT1-F ( D and E ). Transfected cells were seeded on fibronectin-coated glass coverslips for 1 h and treated with anti-FLAG antibody (A–C and E) or anti-CD44 antibody (D) for 30 min at 37°C. After excess antibody had been washed off with PBS, the cells were treated with CyD (1 µg/ml) for 30 min at 37°C. They were then fixed, and the localization of the bound antibody and actin cytoskeleton was visualized with Cy3-conjugated anti-mouse <t>IgG</t> (red) and <t>Alexa488-conjugated</t> phalloidin (A–C and E) or Alexa488-conjugated anti-rabbit IgG and Alexa594-conjugated phalloidin (D), respectively. Scale bar, 10 µm.
    Goat Anti Rabbit Igg Alexa488 Conjugated Secondary, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg alexa488 conjugated secondary/product/Cell Signaling Technology Inc
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    91
    The Jackson Laboratory alexa488 conjugated goat anti rabbit igg
    Fig. 4. Actin cytoskeleton regulates localization of CD44H and MT1-MMP. CHO-K1 cells were transiently transfected with expression plasmids for CD44H-F ( A ), MT1-F ( B ), MT1-F/dPEX ( C ) and CD44H/dCP + MT1-F ( D and E ). Transfected cells were seeded on fibronectin-coated glass coverslips for 1 h and treated with anti-FLAG antibody (A–C and E) or anti-CD44 antibody (D) for 30 min at 37°C. After excess antibody had been washed off with PBS, the cells were treated with CyD (1 µg/ml) for 30 min at 37°C. They were then fixed, and the localization of the bound antibody and actin cytoskeleton was visualized with Cy3-conjugated anti-mouse <t>IgG</t> (red) and <t>Alexa488-conjugated</t> phalloidin (A–C and E) or Alexa488-conjugated anti-rabbit IgG and Alexa594-conjugated phalloidin (D), respectively. Scale bar, 10 µm.
    Alexa488 Conjugated Goat Anti Rabbit Igg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa488 conjugated goat anti rabbit igg/product/The Jackson Laboratory
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    Thermo Fisher goat polyclonal anti rabbit igg alexa488 conjugate
    Fig. 4. Actin cytoskeleton regulates localization of CD44H and MT1-MMP. CHO-K1 cells were transiently transfected with expression plasmids for CD44H-F ( A ), MT1-F ( B ), MT1-F/dPEX ( C ) and CD44H/dCP + MT1-F ( D and E ). Transfected cells were seeded on fibronectin-coated glass coverslips for 1 h and treated with anti-FLAG antibody (A–C and E) or anti-CD44 antibody (D) for 30 min at 37°C. After excess antibody had been washed off with PBS, the cells were treated with CyD (1 µg/ml) for 30 min at 37°C. They were then fixed, and the localization of the bound antibody and actin cytoskeleton was visualized with Cy3-conjugated anti-mouse <t>IgG</t> (red) and <t>Alexa488-conjugated</t> phalloidin (A–C and E) or Alexa488-conjugated anti-rabbit IgG and Alexa594-conjugated phalloidin (D), respectively. Scale bar, 10 µm.
    Goat Polyclonal Anti Rabbit Igg Alexa488 Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal anti rabbit igg alexa488 conjugate/product/Thermo Fisher
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    Thermo Fisher alexa488 conjugated anti goat igg alexa555 conjugated anti rabbit igg
    Fig. 4. Actin cytoskeleton regulates localization of CD44H and MT1-MMP. CHO-K1 cells were transiently transfected with expression plasmids for CD44H-F ( A ), MT1-F ( B ), MT1-F/dPEX ( C ) and CD44H/dCP + MT1-F ( D and E ). Transfected cells were seeded on fibronectin-coated glass coverslips for 1 h and treated with anti-FLAG antibody (A–C and E) or anti-CD44 antibody (D) for 30 min at 37°C. After excess antibody had been washed off with PBS, the cells were treated with CyD (1 µg/ml) for 30 min at 37°C. They were then fixed, and the localization of the bound antibody and actin cytoskeleton was visualized with Cy3-conjugated anti-mouse <t>IgG</t> (red) and <t>Alexa488-conjugated</t> phalloidin (A–C and E) or Alexa488-conjugated anti-rabbit IgG and Alexa594-conjugated phalloidin (D), respectively. Scale bar, 10 µm.
    Alexa488 Conjugated Anti Goat Igg Alexa555 Conjugated Anti Rabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa488 conjugated goat anti rabbit ig
    Alendronate treatment promotes osteocyte apoptosis and stimulates TNFα expression in macrophages. Wild-type mice were administered alendronate for two weeks. Then, osteomyelitis (infection) was established in left femurs, as in Fig. 1 . Right femurs were sham-operated and served as controls (non-infection). Seven days later, bone sections were prepared and labeled with Biotin-dUTP using terminal deoxynucleotidyl trans (TdT), followed by Avidin-DTAF as TUNEL staining (TUNEL) to identify apoptotic cells ( a ). Sections were doubly-stained with <t>Alexa488-conjugated</t> rat anti-mouse F4/80 and goat anti-mouse TNFα, followed by Alexa488-conjugated donkey anti-rat Ig’ and Alexa568-conjugated donkey anti-goat Ig’. ( b ). Nuclei were stained with DAPI. Sections were observed under a fluorescence microscope. Bar = 10 μm. Representative data of at least two independent experiments are shown.
    Alexa488 Conjugated Goat Anti Rabbit Ig, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa488 conjugated goat anti rabbit ig/product/Thermo Fisher
    Average 99 stars, based on 22 article reviews
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    Image Search Results


    Fig. 4. Actin cytoskeleton regulates localization of CD44H and MT1-MMP. CHO-K1 cells were transiently transfected with expression plasmids for CD44H-F ( A ), MT1-F ( B ), MT1-F/dPEX ( C ) and CD44H/dCP + MT1-F ( D and E ). Transfected cells were seeded on fibronectin-coated glass coverslips for 1 h and treated with anti-FLAG antibody (A–C and E) or anti-CD44 antibody (D) for 30 min at 37°C. After excess antibody had been washed off with PBS, the cells were treated with CyD (1 µg/ml) for 30 min at 37°C. They were then fixed, and the localization of the bound antibody and actin cytoskeleton was visualized with Cy3-conjugated anti-mouse IgG (red) and Alexa488-conjugated phalloidin (A–C and E) or Alexa488-conjugated anti-rabbit IgG and Alexa594-conjugated phalloidin (D), respectively. Scale bar, 10 µm.

    Journal: The EMBO Journal

    Article Title: CD44 directs membrane-type 1 matrix metalloproteinase to lamellipodia by associating with its hemopexin-like domain

    doi: 10.1093/emboj/cdf411

    Figure Lengend Snippet: Fig. 4. Actin cytoskeleton regulates localization of CD44H and MT1-MMP. CHO-K1 cells were transiently transfected with expression plasmids for CD44H-F ( A ), MT1-F ( B ), MT1-F/dPEX ( C ) and CD44H/dCP + MT1-F ( D and E ). Transfected cells were seeded on fibronectin-coated glass coverslips for 1 h and treated with anti-FLAG antibody (A–C and E) or anti-CD44 antibody (D) for 30 min at 37°C. After excess antibody had been washed off with PBS, the cells were treated with CyD (1 µg/ml) for 30 min at 37°C. They were then fixed, and the localization of the bound antibody and actin cytoskeleton was visualized with Cy3-conjugated anti-mouse IgG (red) and Alexa488-conjugated phalloidin (A–C and E) or Alexa488-conjugated anti-rabbit IgG and Alexa594-conjugated phalloidin (D), respectively. Scale bar, 10 µm.

    Article Snippet: Rabbit polyclonal antibody against human CD44 was purchased from Santa Cruz (Santa Cruz, CA); mouse anti-FLAG M2 antibody and alkaline phosphatase-conjugated antibodies (goat anti-mouse IgG and goat anti-rabbit IgG) from Sigma; mouse anti-c-Myc antibody from Roche Molecular Biochemicals; goat Cy3-conjugated anti-mouse IgG from Jackson ImmunoResearch Laboratories (USA); goat Alexa488-conjugated anti-rabbit IgG from Molecular Probes (USA); and mouse anti-MT1-MMPPEX monoclonal antibody (222-1D8) and anti-MT1-MMPCAT monoclonal antibody (113-5B7) from Daiichi Fine Chemical Co. (Takaoka, Japan).

    Techniques: Transfection, Expressing

    Fig. 5. MT1-MMP movement by pulling CD44H on the cell surface. A Myc-tagged mutant CD44H lacking the ligand-binding domain (CD44H/dL) was expressed together with either MT1-F ( A and B ), MT1F/dCAT ( C ) or MT1-F/dPEX ( D ) in COS-1 cells. After 48 h transfection, cells on glass coverslips were treated with anti-Myc-conjugated microbeads for 30 min. CD44 was then visualized with anti-CD44 rabbit polyclonal antibody and Alexa488-conjugated anti-rabbit IgG antibody. MT1-F, MT1-F/dCAT and MT1-F/dPEX were visualized with anti-FLAG M2 antibody and Alexa568-conjugated anti-mouse IgG antibody. The boxed areas in the MT1-F column (A) are magnified in the lower column (B). Only magnified pictures are presented for MT1-F/dCAT (C) and MT1-F/dPEX (D). Scale bar, 10 µm.

    Journal: The EMBO Journal

    Article Title: CD44 directs membrane-type 1 matrix metalloproteinase to lamellipodia by associating with its hemopexin-like domain

    doi: 10.1093/emboj/cdf411

    Figure Lengend Snippet: Fig. 5. MT1-MMP movement by pulling CD44H on the cell surface. A Myc-tagged mutant CD44H lacking the ligand-binding domain (CD44H/dL) was expressed together with either MT1-F ( A and B ), MT1F/dCAT ( C ) or MT1-F/dPEX ( D ) in COS-1 cells. After 48 h transfection, cells on glass coverslips were treated with anti-Myc-conjugated microbeads for 30 min. CD44 was then visualized with anti-CD44 rabbit polyclonal antibody and Alexa488-conjugated anti-rabbit IgG antibody. MT1-F, MT1-F/dCAT and MT1-F/dPEX were visualized with anti-FLAG M2 antibody and Alexa568-conjugated anti-mouse IgG antibody. The boxed areas in the MT1-F column (A) are magnified in the lower column (B). Only magnified pictures are presented for MT1-F/dCAT (C) and MT1-F/dPEX (D). Scale bar, 10 µm.

    Article Snippet: Rabbit polyclonal antibody against human CD44 was purchased from Santa Cruz (Santa Cruz, CA); mouse anti-FLAG M2 antibody and alkaline phosphatase-conjugated antibodies (goat anti-mouse IgG and goat anti-rabbit IgG) from Sigma; mouse anti-c-Myc antibody from Roche Molecular Biochemicals; goat Cy3-conjugated anti-mouse IgG from Jackson ImmunoResearch Laboratories (USA); goat Alexa488-conjugated anti-rabbit IgG from Molecular Probes (USA); and mouse anti-MT1-MMPPEX monoclonal antibody (222-1D8) and anti-MT1-MMPCAT monoclonal antibody (113-5B7) from Daiichi Fine Chemical Co. (Takaoka, Japan).

    Techniques: Mutagenesis, Ligand Binding Assay, Transfection

    Fig. 3. Localization of CD44H and MT1-MMP to the lamellipodium. ( A ) HT-1080 cells were cultured on glass coverslips for 12 h. Cells were reacted with rabbit anti-CD44 antibody for 30 min. After a wash with PBS, cells were treated with TPA for 30 min. Following fixation, bound anti-CD44 antibody was visualized with Alexa488-conjugated anti-rabbit IgG. Actin cytoskeleton was identified with Alexa594-conjugated phalloidin. ( B ) HT-1080 cells expressing either MT1-F or MT1-F/dPEX were treated with anti-FLAG antibody for 30 min, followed by TPA. After fixation, antibodies reacting with MT1-F were visualized with Alexa488-conjugated anti-mouse IgG. The expression level of MT1-F and MT1-F/dPEX was confirmed by western blotting. ( C ) A mutant CD44H (CD44H/dCP) that lacks the cytoplasmic domain (as illustrated) was constructed. CHO-K1 cells were transiently transfected with the plasmids indicated and subjected to immunoprecipitation using anti-FLAG M2 antibody in the presence or absence of FLAG peptide (200 μg/ml). Precipitates were analyzed by western blotting using anti-CD44 antibody (2C5). ( D ) MT1-MMP + CD44H-F/dCP or MT1F + CD44H/dCP were expressed in HT-1080 cells. CD44H-F/dCP and MT1-F on the cell surface were visualized using anti-FLAG M2 antibody and Cy3- conjugated anti-mouse IgG. Cells were treated with TPA similarly to (A) before fixation. F-actin was stained with Alexa-594-conjugated phalloidin. Scale bar, 10 µm.

    Journal: The EMBO Journal

    Article Title: CD44 directs membrane-type 1 matrix metalloproteinase to lamellipodia by associating with its hemopexin-like domain

    doi: 10.1093/emboj/cdf411

    Figure Lengend Snippet: Fig. 3. Localization of CD44H and MT1-MMP to the lamellipodium. ( A ) HT-1080 cells were cultured on glass coverslips for 12 h. Cells were reacted with rabbit anti-CD44 antibody for 30 min. After a wash with PBS, cells were treated with TPA for 30 min. Following fixation, bound anti-CD44 antibody was visualized with Alexa488-conjugated anti-rabbit IgG. Actin cytoskeleton was identified with Alexa594-conjugated phalloidin. ( B ) HT-1080 cells expressing either MT1-F or MT1-F/dPEX were treated with anti-FLAG antibody for 30 min, followed by TPA. After fixation, antibodies reacting with MT1-F were visualized with Alexa488-conjugated anti-mouse IgG. The expression level of MT1-F and MT1-F/dPEX was confirmed by western blotting. ( C ) A mutant CD44H (CD44H/dCP) that lacks the cytoplasmic domain (as illustrated) was constructed. CHO-K1 cells were transiently transfected with the plasmids indicated and subjected to immunoprecipitation using anti-FLAG M2 antibody in the presence or absence of FLAG peptide (200 μg/ml). Precipitates were analyzed by western blotting using anti-CD44 antibody (2C5). ( D ) MT1-MMP + CD44H-F/dCP or MT1F + CD44H/dCP were expressed in HT-1080 cells. CD44H-F/dCP and MT1-F on the cell surface were visualized using anti-FLAG M2 antibody and Cy3- conjugated anti-mouse IgG. Cells were treated with TPA similarly to (A) before fixation. F-actin was stained with Alexa-594-conjugated phalloidin. Scale bar, 10 µm.

    Article Snippet: Rabbit polyclonal antibody against human CD44 was purchased from Santa Cruz (Santa Cruz, CA); mouse anti-FLAG M2 antibody and alkaline phosphatase-conjugated antibodies (goat anti-mouse IgG and goat anti-rabbit IgG) from Sigma; mouse anti-c-Myc antibody from Roche Molecular Biochemicals; goat Cy3-conjugated anti-mouse IgG from Jackson ImmunoResearch Laboratories (USA); goat Alexa488-conjugated anti-rabbit IgG from Molecular Probes (USA); and mouse anti-MT1-MMPPEX monoclonal antibody (222-1D8) and anti-MT1-MMPCAT monoclonal antibody (113-5B7) from Daiichi Fine Chemical Co. (Takaoka, Japan).

    Techniques: Cell Culture, Expressing, Western Blot, Mutagenesis, Construct, Transfection, Immunoprecipitation, Staining

    Alendronate treatment promotes osteocyte apoptosis and stimulates TNFα expression in macrophages. Wild-type mice were administered alendronate for two weeks. Then, osteomyelitis (infection) was established in left femurs, as in Fig. 1 . Right femurs were sham-operated and served as controls (non-infection). Seven days later, bone sections were prepared and labeled with Biotin-dUTP using terminal deoxynucleotidyl trans (TdT), followed by Avidin-DTAF as TUNEL staining (TUNEL) to identify apoptotic cells ( a ). Sections were doubly-stained with Alexa488-conjugated rat anti-mouse F4/80 and goat anti-mouse TNFα, followed by Alexa488-conjugated donkey anti-rat Ig’ and Alexa568-conjugated donkey anti-goat Ig’. ( b ). Nuclei were stained with DAPI. Sections were observed under a fluorescence microscope. Bar = 10 μm. Representative data of at least two independent experiments are shown.

    Journal: Scientific Reports

    Article Title: Elevation of pro-inflammatory cytokine levels following anti-resorptive drug treatment is required for osteonecrosis development in infectious osteomyelitis

    doi: 10.1038/srep46322

    Figure Lengend Snippet: Alendronate treatment promotes osteocyte apoptosis and stimulates TNFα expression in macrophages. Wild-type mice were administered alendronate for two weeks. Then, osteomyelitis (infection) was established in left femurs, as in Fig. 1 . Right femurs were sham-operated and served as controls (non-infection). Seven days later, bone sections were prepared and labeled with Biotin-dUTP using terminal deoxynucleotidyl trans (TdT), followed by Avidin-DTAF as TUNEL staining (TUNEL) to identify apoptotic cells ( a ). Sections were doubly-stained with Alexa488-conjugated rat anti-mouse F4/80 and goat anti-mouse TNFα, followed by Alexa488-conjugated donkey anti-rat Ig’ and Alexa568-conjugated donkey anti-goat Ig’. ( b ). Nuclei were stained with DAPI. Sections were observed under a fluorescence microscope. Bar = 10 μm. Representative data of at least two independent experiments are shown.

    Article Snippet: Immunofluorescence Surgical sections of bone tissue were stained using a MEBSTAIN Apoptosis TUNEL Kit Direct (MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., Nagoya, Japan), or stained with anti-single standard DNA (ssDNA) Rabbit IgG (#18731 1:50; IBL, Gunma, Japan) followed by Alexa488-conjugated goat anti-rabbit Ig’ (#A-11034 1:200; Invitrogen, Carlsbad, CA).

    Techniques: Expressing, Mouse Assay, Infection, Labeling, Avidin-Biotin Assay, TUNEL Assay, Staining, Fluorescence, Microscopy