alexa488-conjugated goat anti-mouse igg Search Results


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  • 99
    Thermo Fisher goat anti mouse alexa488 conjugated antibody
    Goat Anti Mouse Alexa488 Conjugated Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse alexa488 conjugated antibody/product/Thermo Fisher
    Average 99 stars, based on 31 article reviews
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    88
    Thermo Fisher goat anti mouse igg conjugated to alexa488
    Precocious Blimp1 expression causes autoimmunity in Prdm1 ihCd2/+ mice with progressing age Presence of <t>IgG</t> antibodies detecting dsDNA, cardiolipin, SSA (Ro‐52), and SSB (La) in the serum of Prdm1 ihCd2/+ (red dots) and wild‐type (gray dots) mice at the age of 2, 4, and 12 months. The titers of the different IgG antibodies were determined in the serum of male and female mice by ELISA and are displayed as arbitrary units (AU). The serum of 6‐month‐old Fasl gld/gld mice was used as positive control. Representative images of anti‐nuclear antibody (ANA) staining obtained with serum from 12‐month‐old Prdm1 ihCd2/+ or wild‐type mice (right), as detected by indirect immunofluorescence assay using HEp‐2 cells and an <t>Alexa488‐conjugated</t> anti‐mouse IgG detection antibody. The serum of Fasl gld/gld mice was used as positive control. The presence of ANA‐IgG antibodies was analyzed for 22 Prdm1 ihCd2/+ and 12 wild‐type mice, as summarized in the pie chart (right). Periodic acid‐Schiff (PAS) staining of paraffin‐embedded kidney sections of Prdm1 ihCd2/+ . Representative pictures of glomeruli of Prdm1 ihCd2/+ ) are shown to the right. Arrows denote intracapillary hyaline “thrombi,” asterisks indicate mesangial sclerosis with obscured capillary loops, and the arrowhead points to an obsolescent glomerulus with collapse of the glomerular tuft architecture. Statistical data (A, C) are shown as mean value with SEM and were analyzed by the Mann–Whitney U ‐test (A) or the Student's t ‐test (C); * P
    Goat Anti Mouse Igg Conjugated To Alexa488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg conjugated to alexa488/product/Thermo Fisher
    Average 88 stars, based on 46 article reviews
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    91
    The Jackson Laboratory alexa488 conjugated goat anti mouse igg
    Precocious Blimp1 expression causes autoimmunity in Prdm1 ihCd2/+ mice with progressing age Presence of <t>IgG</t> antibodies detecting dsDNA, cardiolipin, SSA (Ro‐52), and SSB (La) in the serum of Prdm1 ihCd2/+ (red dots) and wild‐type (gray dots) mice at the age of 2, 4, and 12 months. The titers of the different IgG antibodies were determined in the serum of male and female mice by ELISA and are displayed as arbitrary units (AU). The serum of 6‐month‐old Fasl gld/gld mice was used as positive control. Representative images of anti‐nuclear antibody (ANA) staining obtained with serum from 12‐month‐old Prdm1 ihCd2/+ or wild‐type mice (right), as detected by indirect immunofluorescence assay using HEp‐2 cells and an <t>Alexa488‐conjugated</t> anti‐mouse IgG detection antibody. The serum of Fasl gld/gld mice was used as positive control. The presence of ANA‐IgG antibodies was analyzed for 22 Prdm1 ihCd2/+ and 12 wild‐type mice, as summarized in the pie chart (right). Periodic acid‐Schiff (PAS) staining of paraffin‐embedded kidney sections of Prdm1 ihCd2/+ . Representative pictures of glomeruli of Prdm1 ihCd2/+ ) are shown to the right. Arrows denote intracapillary hyaline “thrombi,” asterisks indicate mesangial sclerosis with obscured capillary loops, and the arrowhead points to an obsolescent glomerulus with collapse of the glomerular tuft architecture. Statistical data (A, C) are shown as mean value with SEM and were analyzed by the Mann–Whitney U ‐test (A) or the Student's t ‐test (C); * P
    Alexa488 Conjugated Goat Anti Mouse Igg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa488 conjugated goat anti mouse igg/product/The Jackson Laboratory
    Average 91 stars, based on 1 article reviews
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    89
    Jackson Immuno alexa488 conjugated goat anti mouse igg
    Precocious Blimp1 expression causes autoimmunity in Prdm1 ihCd2/+ mice with progressing age Presence of <t>IgG</t> antibodies detecting dsDNA, cardiolipin, SSA (Ro‐52), and SSB (La) in the serum of Prdm1 ihCd2/+ (red dots) and wild‐type (gray dots) mice at the age of 2, 4, and 12 months. The titers of the different IgG antibodies were determined in the serum of male and female mice by ELISA and are displayed as arbitrary units (AU). The serum of 6‐month‐old Fasl gld/gld mice was used as positive control. Representative images of anti‐nuclear antibody (ANA) staining obtained with serum from 12‐month‐old Prdm1 ihCd2/+ or wild‐type mice (right), as detected by indirect immunofluorescence assay using HEp‐2 cells and an <t>Alexa488‐conjugated</t> anti‐mouse IgG detection antibody. The serum of Fasl gld/gld mice was used as positive control. The presence of ANA‐IgG antibodies was analyzed for 22 Prdm1 ihCd2/+ and 12 wild‐type mice, as summarized in the pie chart (right). Periodic acid‐Schiff (PAS) staining of paraffin‐embedded kidney sections of Prdm1 ihCd2/+ . Representative pictures of glomeruli of Prdm1 ihCd2/+ ) are shown to the right. Arrows denote intracapillary hyaline “thrombi,” asterisks indicate mesangial sclerosis with obscured capillary loops, and the arrowhead points to an obsolescent glomerulus with collapse of the glomerular tuft architecture. Statistical data (A, C) are shown as mean value with SEM and were analyzed by the Mann–Whitney U ‐test (A) or the Student's t ‐test (C); * P
    Alexa488 Conjugated Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 89/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa488 conjugated goat anti mouse igg/product/Jackson Immuno
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    Cell Signaling Technology Inc alexa488 conjugated goat anti mouse igg
    Precocious Blimp1 expression causes autoimmunity in Prdm1 ihCd2/+ mice with progressing age Presence of <t>IgG</t> antibodies detecting dsDNA, cardiolipin, SSA (Ro‐52), and SSB (La) in the serum of Prdm1 ihCd2/+ (red dots) and wild‐type (gray dots) mice at the age of 2, 4, and 12 months. The titers of the different IgG antibodies were determined in the serum of male and female mice by ELISA and are displayed as arbitrary units (AU). The serum of 6‐month‐old Fasl gld/gld mice was used as positive control. Representative images of anti‐nuclear antibody (ANA) staining obtained with serum from 12‐month‐old Prdm1 ihCd2/+ or wild‐type mice (right), as detected by indirect immunofluorescence assay using HEp‐2 cells and an <t>Alexa488‐conjugated</t> anti‐mouse IgG detection antibody. The serum of Fasl gld/gld mice was used as positive control. The presence of ANA‐IgG antibodies was analyzed for 22 Prdm1 ihCd2/+ and 12 wild‐type mice, as summarized in the pie chart (right). Periodic acid‐Schiff (PAS) staining of paraffin‐embedded kidney sections of Prdm1 ihCd2/+ . Representative pictures of glomeruli of Prdm1 ihCd2/+ ) are shown to the right. Arrows denote intracapillary hyaline “thrombi,” asterisks indicate mesangial sclerosis with obscured capillary loops, and the arrowhead points to an obsolescent glomerulus with collapse of the glomerular tuft architecture. Statistical data (A, C) are shown as mean value with SEM and were analyzed by the Mann–Whitney U ‐test (A) or the Student's t ‐test (C); * P
    Alexa488 Conjugated Goat Anti Mouse Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa488 conjugated goat anti mouse igg/product/Cell Signaling Technology Inc
    Average 89 stars, based on 4 article reviews
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    89
    Becton Dickinson alexa488 conjugated goat anti mouse igg
    Precocious Blimp1 expression causes autoimmunity in Prdm1 ihCd2/+ mice with progressing age Presence of <t>IgG</t> antibodies detecting dsDNA, cardiolipin, SSA (Ro‐52), and SSB (La) in the serum of Prdm1 ihCd2/+ (red dots) and wild‐type (gray dots) mice at the age of 2, 4, and 12 months. The titers of the different IgG antibodies were determined in the serum of male and female mice by ELISA and are displayed as arbitrary units (AU). The serum of 6‐month‐old Fasl gld/gld mice was used as positive control. Representative images of anti‐nuclear antibody (ANA) staining obtained with serum from 12‐month‐old Prdm1 ihCd2/+ or wild‐type mice (right), as detected by indirect immunofluorescence assay using HEp‐2 cells and an <t>Alexa488‐conjugated</t> anti‐mouse IgG detection antibody. The serum of Fasl gld/gld mice was used as positive control. The presence of ANA‐IgG antibodies was analyzed for 22 Prdm1 ihCd2/+ and 12 wild‐type mice, as summarized in the pie chart (right). Periodic acid‐Schiff (PAS) staining of paraffin‐embedded kidney sections of Prdm1 ihCd2/+ . Representative pictures of glomeruli of Prdm1 ihCd2/+ ) are shown to the right. Arrows denote intracapillary hyaline “thrombi,” asterisks indicate mesangial sclerosis with obscured capillary loops, and the arrowhead points to an obsolescent glomerulus with collapse of the glomerular tuft architecture. Statistical data (A, C) are shown as mean value with SEM and were analyzed by the Mann–Whitney U ‐test (A) or the Student's t ‐test (C); * P
    Alexa488 Conjugated Goat Anti Mouse Igg, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa488 conjugated goat anti mouse igg/product/Becton Dickinson
    Average 89 stars, based on 15 article reviews
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    88
    Millipore goat anti mouse igg conjugated to alexa488
    Dopamine receptor activation decreases the cell-surface expression of GABA A receptors via a dynamin-dependent pathway. A , B , Internalization of GABA A receptors was visualized by staining with β 2/3 -specific antibody in the presence of vehicle, SKF-38393 ( A ), or quinpirole ( B ), as described in Materials and Methods. Binding of β 2/3 -specific antibody to the surface expressed GABA A receptors was visualized using a secondary anti-mouse <t>IgG</t> coupled to Alexa555 (in red), while binding of the same antibody to internalized receptors was visualized using a secondary anti-mouse IgG coupled to <t>Alexa488</t> (in green). Scale bars correspond to 10 μm. C , The decrease in surface β 2/3 subunit levels caused by SKF-38393 (SKF) was significantly attenuated in the presence of a dynamin inhibitory peptide (SKF/DynP), but not in the presence of its scrambled control (SKF/DynPC). D , The decrease in surface β 2/3 subunit levels caused by quinpirole (Q) was also significantly attenuated in the presence of a dynamin inhibitory peptide (Q/DynP), but not by its scrambled control (Q/DynPC). Bars represent mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett post hoc analysis versus control (* p
    Goat Anti Mouse Igg Conjugated To Alexa488, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher goat anti mouse alexa488 conjugated iggs
    Dopamine receptor activation decreases the cell-surface expression of GABA A receptors via a dynamin-dependent pathway. A , B , Internalization of GABA A receptors was visualized by staining with β 2/3 -specific antibody in the presence of vehicle, SKF-38393 ( A ), or quinpirole ( B ), as described in Materials and Methods. Binding of β 2/3 -specific antibody to the surface expressed GABA A receptors was visualized using a secondary anti-mouse <t>IgG</t> coupled to Alexa555 (in red), while binding of the same antibody to internalized receptors was visualized using a secondary anti-mouse IgG coupled to <t>Alexa488</t> (in green). Scale bars correspond to 10 μm. C , The decrease in surface β 2/3 subunit levels caused by SKF-38393 (SKF) was significantly attenuated in the presence of a dynamin inhibitory peptide (SKF/DynP), but not in the presence of its scrambled control (SKF/DynPC). D , The decrease in surface β 2/3 subunit levels caused by quinpirole (Q) was also significantly attenuated in the presence of a dynamin inhibitory peptide (Q/DynP), but not by its scrambled control (Q/DynPC). Bars represent mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett post hoc analysis versus control (* p
    Goat Anti Mouse Alexa488 Conjugated Iggs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse alexa488 conjugated iggs/product/Thermo Fisher
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    Thermo Fisher goat polyclonal anti mouse igg alexa488 conjugate
    Dopamine receptor activation decreases the cell-surface expression of GABA A receptors via a dynamin-dependent pathway. A , B , Internalization of GABA A receptors was visualized by staining with β 2/3 -specific antibody in the presence of vehicle, SKF-38393 ( A ), or quinpirole ( B ), as described in Materials and Methods. Binding of β 2/3 -specific antibody to the surface expressed GABA A receptors was visualized using a secondary anti-mouse <t>IgG</t> coupled to Alexa555 (in red), while binding of the same antibody to internalized receptors was visualized using a secondary anti-mouse IgG coupled to <t>Alexa488</t> (in green). Scale bars correspond to 10 μm. C , The decrease in surface β 2/3 subunit levels caused by SKF-38393 (SKF) was significantly attenuated in the presence of a dynamin inhibitory peptide (SKF/DynP), but not in the presence of its scrambled control (SKF/DynPC). D , The decrease in surface β 2/3 subunit levels caused by quinpirole (Q) was also significantly attenuated in the presence of a dynamin inhibitory peptide (Q/DynP), but not by its scrambled control (Q/DynPC). Bars represent mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett post hoc analysis versus control (* p
    Goat Polyclonal Anti Mouse Igg Alexa488 Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal anti mouse igg alexa488 conjugate/product/Thermo Fisher
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    Thermo Fisher facs goat anti mouse igg alexa488 conjugate
    Dopamine receptor activation decreases the cell-surface expression of GABA A receptors via a dynamin-dependent pathway. A , B , Internalization of GABA A receptors was visualized by staining with β 2/3 -specific antibody in the presence of vehicle, SKF-38393 ( A ), or quinpirole ( B ), as described in Materials and Methods. Binding of β 2/3 -specific antibody to the surface expressed GABA A receptors was visualized using a secondary anti-mouse <t>IgG</t> coupled to Alexa555 (in red), while binding of the same antibody to internalized receptors was visualized using a secondary anti-mouse IgG coupled to <t>Alexa488</t> (in green). Scale bars correspond to 10 μm. C , The decrease in surface β 2/3 subunit levels caused by SKF-38393 (SKF) was significantly attenuated in the presence of a dynamin inhibitory peptide (SKF/DynP), but not in the presence of its scrambled control (SKF/DynPC). D , The decrease in surface β 2/3 subunit levels caused by quinpirole (Q) was also significantly attenuated in the presence of a dynamin inhibitory peptide (Q/DynP), but not by its scrambled control (Q/DynPC). Bars represent mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett post hoc analysis versus control (* p
    Facs Goat Anti Mouse Igg Alexa488 Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Precocious Blimp1 expression causes autoimmunity in Prdm1 ihCd2/+ mice with progressing age Presence of IgG antibodies detecting dsDNA, cardiolipin, SSA (Ro‐52), and SSB (La) in the serum of Prdm1 ihCd2/+ (red dots) and wild‐type (gray dots) mice at the age of 2, 4, and 12 months. The titers of the different IgG antibodies were determined in the serum of male and female mice by ELISA and are displayed as arbitrary units (AU). The serum of 6‐month‐old Fasl gld/gld mice was used as positive control. Representative images of anti‐nuclear antibody (ANA) staining obtained with serum from 12‐month‐old Prdm1 ihCd2/+ or wild‐type mice (right), as detected by indirect immunofluorescence assay using HEp‐2 cells and an Alexa488‐conjugated anti‐mouse IgG detection antibody. The serum of Fasl gld/gld mice was used as positive control. The presence of ANA‐IgG antibodies was analyzed for 22 Prdm1 ihCd2/+ and 12 wild‐type mice, as summarized in the pie chart (right). Periodic acid‐Schiff (PAS) staining of paraffin‐embedded kidney sections of Prdm1 ihCd2/+ . Representative pictures of glomeruli of Prdm1 ihCd2/+ ) are shown to the right. Arrows denote intracapillary hyaline “thrombi,” asterisks indicate mesangial sclerosis with obscured capillary loops, and the arrowhead points to an obsolescent glomerulus with collapse of the glomerular tuft architecture. Statistical data (A, C) are shown as mean value with SEM and were analyzed by the Mann–Whitney U ‐test (A) or the Student's t ‐test (C); * P

    Journal: The EMBO Journal

    Article Title: Precocious expression of Blimp1 in B cells causes autoimmune disease with increased self‐reactive plasma cells

    doi: 10.15252/embj.2018100010

    Figure Lengend Snippet: Precocious Blimp1 expression causes autoimmunity in Prdm1 ihCd2/+ mice with progressing age Presence of IgG antibodies detecting dsDNA, cardiolipin, SSA (Ro‐52), and SSB (La) in the serum of Prdm1 ihCd2/+ (red dots) and wild‐type (gray dots) mice at the age of 2, 4, and 12 months. The titers of the different IgG antibodies were determined in the serum of male and female mice by ELISA and are displayed as arbitrary units (AU). The serum of 6‐month‐old Fasl gld/gld mice was used as positive control. Representative images of anti‐nuclear antibody (ANA) staining obtained with serum from 12‐month‐old Prdm1 ihCd2/+ or wild‐type mice (right), as detected by indirect immunofluorescence assay using HEp‐2 cells and an Alexa488‐conjugated anti‐mouse IgG detection antibody. The serum of Fasl gld/gld mice was used as positive control. The presence of ANA‐IgG antibodies was analyzed for 22 Prdm1 ihCd2/+ and 12 wild‐type mice, as summarized in the pie chart (right). Periodic acid‐Schiff (PAS) staining of paraffin‐embedded kidney sections of Prdm1 ihCd2/+ . Representative pictures of glomeruli of Prdm1 ihCd2/+ ) are shown to the right. Arrows denote intracapillary hyaline “thrombi,” asterisks indicate mesangial sclerosis with obscured capillary loops, and the arrowhead points to an obsolescent glomerulus with collapse of the glomerular tuft architecture. Statistical data (A, C) are shown as mean value with SEM and were analyzed by the Mann–Whitney U ‐test (A) or the Student's t ‐test (C); * P

    Article Snippet: For detection of mouse IgM or IgG, the slides were incubated for 30 min at 22°C with an Alexa488‐conjugated goat anti‐mouse IgM antibody or an Alexa488‐conjugated goat anti‐mouse IgG (H + L) antibody (both from Thermo Fisher Scientific) as a secondary antibody.

    Techniques: Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay, Positive Control, Staining, Immunofluorescence, MANN-WHITNEY

    SplitCore-SplitGFP-GB1: Triple-layer fluorescent particles with exposed targeting domains. (a) Design of SplitCore-splitGFP-GB1 fusions (SCSGFP-GB1). GB1 fused to one of the new ends provided by the split GFP insert (see Supplementary Fig. S3 online) should yield fluorescent CLPs with exposed immunoglobulin binding GB1 domains. (b) Expression and CLP formation. Bacterial lysates were subjected to sedimentation as in Fig. 1c . SDS-PAGE (top) demonstrated cosedimentation of two proteins of the expected sizes into fractions 8 to 12 which produced distinct green fluorescent bands in NAGE (UV) that also stained with CB. Similar results were obtained with GB1 fused to GFPβ1-10 on coreN (not shown). (c) Visualization of folded GFP containing material in the gradient tube from which the samples in (b) were derived. (d) Negative staining EM. The two micrographs are from independent grids onto which material from fraction 8, diluted 10-fold in PBS, was applied. Smaller (*; diameter ∼38 nm) and larger (**; diameter ∼42 nm) particles were observed. (e,f) SCSGFP-GB1 binds antibodies. (e) Indirect immunofluorescence. Permeabilized HeLa cells were incubated with mouse anti-tubulin antibody (i, iii) or not (ii, iv), then with SCSGFP-GB1 (i, ii) or an Alexa488-conjugated goat anti-mouse IgG (gαm-Alexa488) antibody (iii, iv). Both generated similar, primary antibody-dependent staining patterns. (f) Fluorescent Western blot. Decreasing amounts of GFP were separated by SDS-PAGE, blotted on nitrocellulose and incubated with a mouse anti-GFP mAb mixture (Roche), then with either gαm-Alexa488 or SC-SGFP-GB1. Fluorescent signals were detected using a Typhoon 7000 imager (GE Healthcare).

    Journal: Scientific Reports

    Article Title: SplitCore: An exceptionally versatile viral nanoparticle for native whole protein display regardless of 3D structure

    doi: 10.1038/srep00005

    Figure Lengend Snippet: SplitCore-SplitGFP-GB1: Triple-layer fluorescent particles with exposed targeting domains. (a) Design of SplitCore-splitGFP-GB1 fusions (SCSGFP-GB1). GB1 fused to one of the new ends provided by the split GFP insert (see Supplementary Fig. S3 online) should yield fluorescent CLPs with exposed immunoglobulin binding GB1 domains. (b) Expression and CLP formation. Bacterial lysates were subjected to sedimentation as in Fig. 1c . SDS-PAGE (top) demonstrated cosedimentation of two proteins of the expected sizes into fractions 8 to 12 which produced distinct green fluorescent bands in NAGE (UV) that also stained with CB. Similar results were obtained with GB1 fused to GFPβ1-10 on coreN (not shown). (c) Visualization of folded GFP containing material in the gradient tube from which the samples in (b) were derived. (d) Negative staining EM. The two micrographs are from independent grids onto which material from fraction 8, diluted 10-fold in PBS, was applied. Smaller (*; diameter ∼38 nm) and larger (**; diameter ∼42 nm) particles were observed. (e,f) SCSGFP-GB1 binds antibodies. (e) Indirect immunofluorescence. Permeabilized HeLa cells were incubated with mouse anti-tubulin antibody (i, iii) or not (ii, iv), then with SCSGFP-GB1 (i, ii) or an Alexa488-conjugated goat anti-mouse IgG (gαm-Alexa488) antibody (iii, iv). Both generated similar, primary antibody-dependent staining patterns. (f) Fluorescent Western blot. Decreasing amounts of GFP were separated by SDS-PAGE, blotted on nitrocellulose and incubated with a mouse anti-GFP mAb mixture (Roche), then with either gαm-Alexa488 or SC-SGFP-GB1. Fluorescent signals were detected using a Typhoon 7000 imager (GE Healthcare).

    Article Snippet: Immunoglobulin binding by SC-SGFP-GB1 CLPs For indirect immunofluorescence, 4% paraformaldehyde fixed HeLa cells were permeabilized using 0.2 % Triton X-100 in PBS, blocked with 5% BSA in PBS and incubated with 0.05 µg/ml of mouse-anti-tubulin mAb, or not; for detection, either 0.13 µM goat-anti-mouse IgG antibody conjugated to Alexa488 (gαm-Alexa488; Invitrogen) or 0.05 µM SC-SGFP-GB1 CLPs were used.

    Techniques: Binding Assay, Expressing, Sedimentation, SDS Page, Produced, Staining, Derivative Assay, Negative Staining, Immunofluorescence, Incubation, Generated, Western Blot

    Dopamine receptor activation decreases the cell-surface expression of GABA A receptors via a dynamin-dependent pathway. A , B , Internalization of GABA A receptors was visualized by staining with β 2/3 -specific antibody in the presence of vehicle, SKF-38393 ( A ), or quinpirole ( B ), as described in Materials and Methods. Binding of β 2/3 -specific antibody to the surface expressed GABA A receptors was visualized using a secondary anti-mouse IgG coupled to Alexa555 (in red), while binding of the same antibody to internalized receptors was visualized using a secondary anti-mouse IgG coupled to Alexa488 (in green). Scale bars correspond to 10 μm. C , The decrease in surface β 2/3 subunit levels caused by SKF-38393 (SKF) was significantly attenuated in the presence of a dynamin inhibitory peptide (SKF/DynP), but not in the presence of its scrambled control (SKF/DynPC). D , The decrease in surface β 2/3 subunit levels caused by quinpirole (Q) was also significantly attenuated in the presence of a dynamin inhibitory peptide (Q/DynP), but not by its scrambled control (Q/DynPC). Bars represent mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett post hoc analysis versus control (* p

    Journal: The Journal of Neuroscience

    Article Title: Dopamine-Dependent Tuning of Striatal Inhibitory Synaptogenesis

    doi: 10.1523/JNEUROSCI.4411-09.2010

    Figure Lengend Snippet: Dopamine receptor activation decreases the cell-surface expression of GABA A receptors via a dynamin-dependent pathway. A , B , Internalization of GABA A receptors was visualized by staining with β 2/3 -specific antibody in the presence of vehicle, SKF-38393 ( A ), or quinpirole ( B ), as described in Materials and Methods. Binding of β 2/3 -specific antibody to the surface expressed GABA A receptors was visualized using a secondary anti-mouse IgG coupled to Alexa555 (in red), while binding of the same antibody to internalized receptors was visualized using a secondary anti-mouse IgG coupled to Alexa488 (in green). Scale bars correspond to 10 μm. C , The decrease in surface β 2/3 subunit levels caused by SKF-38393 (SKF) was significantly attenuated in the presence of a dynamin inhibitory peptide (SKF/DynP), but not in the presence of its scrambled control (SKF/DynPC). D , The decrease in surface β 2/3 subunit levels caused by quinpirole (Q) was also significantly attenuated in the presence of a dynamin inhibitory peptide (Q/DynP), but not by its scrambled control (Q/DynPC). Bars represent mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett post hoc analysis versus control (* p

    Article Snippet: To label internalized GABAA receptors, cultures were incubated with goat anti-mouse IgG conjugated to Alexa488 (3 μg/ml, Millipore Bioscience Research Reagents) for 60 min at the room temperature.

    Techniques: Activation Assay, Expressing, Staining, Binding Assay