Journal: Rapid Communications in Mass Spectrometry

Article Title: Stable Nitrogen Isotope Analysis of Amino Acids by Orbitrap Mass Spectrometry: Application for Extraterrestrial Samples

doi: 10.1002/rcm.10127

Figure Lengend Snippet: Chromatograms of the derivatized amino acid standards (top), Murchison (middle), and procedural blank (bottom) obtained with the GC‐Orbitrap‐IRMS mode. (1) α‐aminoisobutyric acid, (2) L‐isovaline, (3) D‐isovaline, (4) D‐alanine, (5) D‐valine, (6) L‐alanine, (7) D‐α‐aminobutyric acid, (8) L‐valine, (9) L‐ α‐aminobutyric acid, (10) glycine, (11) β‐alanine, (12) D‐aspartic acid, (13) L‐aspartic acid, (14) γ‐aminobutyric acid, (15) D‐glutamic acid, (16) L‐glutamic acid, (17) δ‐amino‐ n ‐valeric acid, (18) ε‐amino‐ n ‐caproic acid. Unlabeled peaks were unidentified.

Article Snippet: L‐valine (USGS75), D‐valine (≥ 98%, Sigma‐Aldrich), glycine (≥ 99%, Sigma‐Aldrich), β‐alanine (99%, Thermo Scientific), L‐alanine (Arndt Schimmelmann, Indiana University), and D‐alanine (≥ 98%, Merck) were treated as unknown samples.

Techniques:

Journal: Rapid Communications in Mass Spectrometry

Article Title: Stable Nitrogen Isotope Analysis of Amino Acids by Orbitrap Mass Spectrometry: Application for Extraterrestrial Samples

doi: 10.1002/rcm.10127

Figure Lengend Snippet: Plot of δ 15 N (‰, Air) values obtained for glycine, β‐alanine, and α‐aminoisobutyric acid (α‐AIB) using the Orbitrap‐IRMS (red dots), compared with values from studies by Engel and Macko (black squares) and Elsila et al. (black triangles) on the Murchison meteorite.

Article Snippet: L‐valine (USGS75), D‐valine (≥ 98%, Sigma‐Aldrich), glycine (≥ 99%, Sigma‐Aldrich), β‐alanine (99%, Thermo Scientific), L‐alanine (Arndt Schimmelmann, Indiana University), and D‐alanine (≥ 98%, Merck) were treated as unknown samples.

Techniques:

Journal: Immunity

Article Title: Loss of CD4 + T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection

doi: 10.1016/j.immuni.2023.07.014

Figure Lengend Snippet: (A and B) Volcano plot of (A) differential metabolite abundance in in vitro -activated (22–24 h) CD4 + T cells from WT and Arg1 CKO mice, n = 4–6. Positive and negative ionization mode features (gray), unannotated features that exceeded log 2 FC > 0.26 (~20% change) and adjusted p value < 0.05 (black), annotated features which exceeded log 2 FC > 0.26 and adjusted p value < 0.05 (red- and blue-highlighted and labeled for clarity), and (B) glutamine abundancy. (C) Simplified schematic of intersecting arginine and glutamine pathways. (D and E) Abundance of (D) glutamate and (E) α-ketoglutarate (αKG). For αKG, n = 3 individual mice shown with technical replicates. (F) Schematic of Gpt2 activity in glutamine metabolism. (G and H) Representative FACS plots showing glutamate pyruvate transaminase 2 (GPT2) protein expression in WT, (G) Arg1 KO, and (H) Arg2 KO CD4 + T cells on day 3 post in vitro activation. (I) Percentage of IFN-γ + , IL-10 + , and IFN-γ-IL-10 double-positive T cells assessed via flow cytometry after in vitro stimulation with or without AOA treatment, n = 2–4 (representative of two independent experiments). *p < 0.05, **p < 0.01, ****p < 0.0001. (B, D, E, G, and I) Two-tailed Student’s t test; (L) one-way ANOVA. ns, no statistically significant difference, TCA cycle, tricarboxylic acid cycle. See also .

Article Snippet: anti-mouse GPT2 , Proteintech/Invitrogen , Cat# 16757-1-AP; RRID: AB_2112098.

Techniques: In Vitro, Labeling, Activity Assay, Expressing, Activation Assay, Flow Cytometry, Two Tailed Test

Journal: Immunity

Article Title: Loss of CD4 + T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection

doi: 10.1016/j.immuni.2023.07.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: anti-mouse GPT2 , Proteintech/Invitrogen , Cat# 16757-1-AP; RRID: AB_2112098.

Techniques: Virus, Isolation, Recombinant, Staining, Cell Isolation, Flow Cytometry, RNA Sequencing Assay, In Vitro, Microarray, CRISPR, Software