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Biosynth Carbosynth
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Biosynth Carbosynth
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Lycopene Inhibits Oxidative Stress-Mediated Inflammatory Responses in Ethanol/Palmitoleic Acid-Stimulated Pancreatic Acinar AR42J Cells
doi: 10.3390/ijms22042101
Figure Lengend Snippet: Lycopene inhibits ethanol and palmitoleic acid (EtOH/POA)-induced zymogen activation in AR42J cells. Cells were pretreated with the indicated concentrations of lycopene for 2 h and then stimulated with EtOH/POA for 20 min. ( A ) Trypsin activity was assayed using a fluorogenic assay with a trypsin-specific substrate. ( B ) Chymotrypsin activity was assayed using a fluorogenic assay with a chymotrypsin-specific substrate. The activity of trypsin or chymotrypsin in untreated cells was set as 100%. Data are expressed as the mean ± standard error (SE) of three different experiments. For each experiment, the number of samples in each group was 4 ( n = 4 per each group). * p < 0.05 vs. untreated cells; + p < 0.05 vs. cells with EtOH/POA stimulation alone (control).
Article Snippet: Chymotrypsin activity was measured using a fluorogenic assay with a substrate specific for
Techniques: Activation Assay, Activity Assay, Control
Journal: International Journal of Molecular Sciences
Article Title: Lycopene Inhibits Oxidative Stress-Mediated Inflammatory Responses in Ethanol/Palmitoleic Acid-Stimulated Pancreatic Acinar AR42J Cells
doi: 10.3390/ijms22042101
Figure Lengend Snippet: NADPH oxidase 1 inhibitor ML171 and antioxidant N-acetylcysteine (NAC) inhibit ethanol and palmitoleic acid (EtOH/POA)-induced zymogen activation and IL-6 expression in AR42J cells. The cells were pretreated with 2 μM ML171 or 1 mM NAC for 1 h, and then stimulated with EtOH/POA for 20 min ( A , B ), 4 h ( C ), and 24 h ( D ). ( A ) Trypsin activity was assayed using a fluorogenic assay with a trypsin-specific substrate. ( B ) Chymotrypsin activity was assayed using a fluorogenic assay with a chymotrypsin-specific substrate. ( C ) mRNA expression of IL-6 was determined by real-time polymerase chain reaction (PCR) and normalized to β-actin. ( D ) Levels of IL-6 in the culture medium were measured by enzyme-linked immunosorbent assay (ELISA). The activity of trypsin or chymotrypsin in untreated cells was set as 100% ( A , B ). IL-6 mRNA level in untreated cells was set as 1 ( C ). Data are expressed as the mean ± standard error (SE) of three different experiments. For each experiment, the number of samples in each group was 4 ( n = 4 per each group). * p < 0.05 vs. untreated cells; + p < 0.05 vs. cells with EtOH/POA stimulation alone (control).
Article Snippet: Chymotrypsin activity was measured using a fluorogenic assay with a substrate specific for
Techniques: Activation Assay, Expressing, Activity Assay, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control