Journal: International Journal of Molecular Sciences

Article Title: Comparison of Bioluminescent Substrates in Natural Infection Models of Neglected Parasitic Diseases

doi: 10.3390/ijms232416074

Figure Lengend Snippet: In vitro bioluminescence of different concentrations of D-luciferin, CycLuc1 and AkaLumine-HCl. ( A ) In vitro bioluminescent signal of L. infantum LLM-2346 PpyRE9 promastigotes and ( B ) L. major JISH118 PpyRE9 promastigotes; ( C ) In vitro bioluminescent signal of L. infantum LLM-2346 PpyRE9 amastigote-infected mouse peritoneal macrophages and ( D ) L. major JISH118 PpyRE9 amastigote-infected mouse peritoneal macrophages; ( E ) In vitro bioluminescent signal of T. brucei brucei AnTAR1.1 PpyRE9 parasites. The representative figures show the average luminescence (expressed in relative luminescence units (RLU) [p/s/cm 2 /Sr]) produced in at least two independent repeat experiments, each conducted in triplicate. Dotted lines represent the background signal. Results are expressed as mean luminescent signal ± SD (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 and **** p ≤ 0.0001).

Article Snippet: One hour post infection (hpi) and 1 day post infection (dpi) mice were anesthetised via 2% isoflurane inhalation and imaged at for 15 min starting 3 min after i.p. injection of 150 mg/kg D-luciferin (Beetle Luciferin Potassium Salt, Promega Benelux, Leiden, The Netherlands) or 7.5 mg/kg CycLuc1 (Merck Life Science B.V., Amsterdam, The Nederlands) or 50 mg/kg AkaLumine-HCl (Merck Life Science B.V., Amsterdam, The Nederlands).

Techniques: In Vitro, Infection, Produced