Journal: Frontiers in Cellular Neuroscience

Article Title: Comparative morphology and plant volatile responses of antennal sensilla in Cinara cedri (Hemiptera: Lachninae), Eriosoma lanigerum (Hemiptera: Eriosomatinae), and Therioaphis trifolii (Hemiptera: Calaphidinae)

doi: 10.3389/fncel.2023.1162349

Figure Lengend Snippet: Chemicals used for single sensillum recording.

Article Snippet: Allyl isothiocyanate , 57-06-25 , >95 , ChromaDex.

Techniques:

Journal: Frontiers in Cellular Neuroscience

Article Title: Comparative morphology and plant volatile responses of antennal sensilla in Cinara cedri (Hemiptera: Lachninae), Eriosoma lanigerum (Hemiptera: Eriosomatinae), and Therioaphis trifolii (Hemiptera: Calaphidinae)

doi: 10.3389/fncel.2023.1162349

Figure Lengend Snippet: Heat map of functional profiles of ORNs housed in primary rhinaria in C. cedri, E. lanigerum , and T. trifolii to the tested odorants. The response intensity of ORNs is color-coded according to the response values ( n = 4–16). The solvent paraffin oil was set as the control. Chemicals were listed in . E2He, trans -2-hexenal; MeSA, methyl salicylate; AITC, allyl isothiocyanate, GA, geranyl acetate.

Article Snippet: Allyl isothiocyanate , 57-06-25 , >95 , ChromaDex.

Techniques: Functional Assay, Solvent, Control

Journal: Frontiers in Cellular Neuroscience

Article Title: Comparative morphology and plant volatile responses of antennal sensilla in Cinara cedri (Hemiptera: Lachninae), Eriosoma lanigerum (Hemiptera: Eriosomatinae), and Therioaphis trifolii (Hemiptera: Calaphidinae)

doi: 10.3389/fncel.2023.1162349

Figure Lengend Snippet: SSR response values of ORNs in antennal placoid sensilla of C. cedri to the tested odorants. (A) Neuronal responses of LP4, LP5I, LP5II, and LP6 of C. cedri . Bars labeled with different letters are significantly different (mean ± SEM, n = 4–16, GLM followed by Duncan's multiple range test). (B) Representative SSR traces showing responses of ORNs in LP4, LP5I, LP5II, and LP6 of C. cedri to (±)-citronellal, (+)-limonene, (–)-β-pinene, E2He, GA, and nepetalactol. Paraffin oil was used as a control. Chemicals were tested at a dose of 10 −3 g. Red bars indicate the 300 ms stimulation of odorants. E2He, trans -2-hexenal; MeSA, methyl salicylate; AITC, allyl isothiocyanate; GA, geranyl acetate.

Article Snippet: Allyl isothiocyanate , 57-06-25 , >95 , ChromaDex.

Techniques: Labeling, Control

Journal: Frontiers in Cellular Neuroscience

Article Title: Comparative morphology and plant volatile responses of antennal sensilla in Cinara cedri (Hemiptera: Lachninae), Eriosoma lanigerum (Hemiptera: Eriosomatinae), and Therioaphis trifolii (Hemiptera: Calaphidinae)

doi: 10.3389/fncel.2023.1162349

Figure Lengend Snippet: SSR response values of ORNs in antennal placoid sensilla of E. lanigerum and T. trifolii to the tested odorants. (A) Neuronal responses of LP5 and LP6 of E. lanigerum ( n = 3–10). Error bars indicate SEM (Student's t -test, * P < 0.05, ** P < 0.01, *** P < 0.001, n.s. represents no significant differences). (B) Neuronal responses of LP5 and LP6 of T. trifolii ( n = 3–11). (C) The representative SSR traces showing ORNs in LP6 and LP5 of E. lanigerum were tested by sabinene, β-citronellol, (–)-linalool, α-terpineol and (–)-piperitone. (D) Representative SSR traces showing responses of ORNs in LP6 and LP5 of T. trifolii to MeSA. Paraffin oil was used as a control. Chemicals were tested at a dose of 10 −3 g. Red bars indicate the 300 ms stimulation of odorants. E2He, trans -2-hexenal; MeSA, methyl salicylate; AITC, allyl isothiocyanate; GA, geranyl acetate.

Article Snippet: Allyl isothiocyanate , 57-06-25 , >95 , ChromaDex.

Techniques: Control

Journal: Frontiers in Cellular Neuroscience

Article Title: Comparative morphology and plant volatile responses of antennal sensilla in Cinara cedri (Hemiptera: Lachninae), Eriosoma lanigerum (Hemiptera: Eriosomatinae), and Therioaphis trifolii (Hemiptera: Calaphidinae)

doi: 10.3389/fncel.2023.1162349

Figure Lengend Snippet: Comparison of the neuronal response values between LP5 and LP6 among C. cedri, E. lanigerum , and T. trifolii to the tested odorants. Bars labeled with different letters are significantly different; n.s. indicates no significant differences (mean ± SEM, n = 3–13, GLM followed by Duncan's multiple range test). E2He, trans -2-hexenal; MeSA, methyl salicylate; AITC, allyl isothiocyanate; GA, geranyl acetate.

Article Snippet: Allyl isothiocyanate , 57-06-25 , >95 , ChromaDex.

Techniques: Comparison, Labeling

Journal: International Journal of Molecular Sciences

Article Title: Allyl-, Butyl- and Phenylethyl-Isothiocyanate Modulate Akt–mTOR and Cyclin–CDK Signaling in Gemcitabine- and Cisplatin-Resistant Bladder Cancer Cell Lines

doi: 10.3390/ijms231910996

Figure Lengend Snippet: Schedule of PEITC, BITC and AITC generation by enzymatic hydrolysis.

Article Snippet: Bladder cancer cells (50 μL, 1 × 105 cells/mL) were treated with AITC, BITC or PEITC (all from Merck, Darmstadt, Germany) at different concentrations (versus non-treated) and then seeded onto 96-well tissue culture plates.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Allyl-, Butyl- and Phenylethyl-Isothiocyanate Modulate Akt–mTOR and Cyclin–CDK Signaling in Gemcitabine- and Cisplatin-Resistant Bladder Cancer Cell Lines

doi: 10.3390/ijms231910996

Figure Lengend Snippet: ( A – C ) Influence of BITC ( A ), AITC ( B ) and PEITC ( C ) on growth of parental, cisplatin- and gemcitabine-resistant RT112, T24, and TCCSUP bladder cancer cell lines. Cell number was evaluated after 24, 48, and 72 h by the MTT assay. Error bars indicate standard deviation (SD), n = 5. * indicates a significant difference to the non-treated control.

Article Snippet: Bladder cancer cells (50 μL, 1 × 105 cells/mL) were treated with AITC, BITC or PEITC (all from Merck, Darmstadt, Germany) at different concentrations (versus non-treated) and then seeded onto 96-well tissue culture plates.

Techniques: MTT Assay, Standard Deviation, Control

Journal: International Journal of Molecular Sciences

Article Title: Allyl-, Butyl- and Phenylethyl-Isothiocyanate Modulate Akt–mTOR and Cyclin–CDK Signaling in Gemcitabine- and Cisplatin-Resistant Bladder Cancer Cell Lines

doi: 10.3390/ijms231910996

Figure Lengend Snippet: Early apoptosis, late apoptosis and necrosis of parental and resistant RT112 ( A ) and T24 cells ( B ) treated with BITC, AITC or PEITC for 72 h. Controls (Ctrl) remained untreated. Upper left quadrants show the percentage of cells in necrosis, upper right quadrants show the percentage of cells in late apoptosis, lower right quadrants show the percentage of cells in early apoptosis and lower left quadrants vital cells (one representative of 3 analyses; SD intra-assay < 10%). A total of 10,000 events were collected for each sample.

Article Snippet: Bladder cancer cells (50 μL, 1 × 105 cells/mL) were treated with AITC, BITC or PEITC (all from Merck, Darmstadt, Germany) at different concentrations (versus non-treated) and then seeded onto 96-well tissue culture plates.

Techniques: Intra Assay

Journal: International Journal of Molecular Sciences

Article Title: Allyl-, Butyl- and Phenylethyl-Isothiocyanate Modulate Akt–mTOR and Cyclin–CDK Signaling in Gemcitabine- and Cisplatin-Resistant Bladder Cancer Cell Lines

doi: 10.3390/ijms231910996

Figure Lengend Snippet: Influence of BITC (7.5 µM), AITC (20 µM) and PEITC (7.5 µM) on clone formation of parental RT112 and T24 cells. Controls were without drugs. Scale bar: 100 µm.

Article Snippet: Bladder cancer cells (50 μL, 1 × 105 cells/mL) were treated with AITC, BITC or PEITC (all from Merck, Darmstadt, Germany) at different concentrations (versus non-treated) and then seeded onto 96-well tissue culture plates.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Allyl-, Butyl- and Phenylethyl-Isothiocyanate Modulate Akt–mTOR and Cyclin–CDK Signaling in Gemcitabine- and Cisplatin-Resistant Bladder Cancer Cell Lines

doi: 10.3390/ijms231910996

Figure Lengend Snippet: Number of RT112 ( A ), T24 ( B ), and TCCSUP ( C ) clones (parental, cisplatin-resistant, gemcitabine-resistant) exposed to BITC (7.5 µM), AITC (20, 40 µM) or PEITC (7.5 µM), indicated as stripped bars. Controls remained untreated (white bars), n = 3. * indicates a significant difference to untreated controls.

Article Snippet: Bladder cancer cells (50 μL, 1 × 105 cells/mL) were treated with AITC, BITC or PEITC (all from Merck, Darmstadt, Germany) at different concentrations (versus non-treated) and then seeded onto 96-well tissue culture plates.

Techniques: Clone Assay

Journal: International Journal of Molecular Sciences

Article Title: Allyl-, Butyl- and Phenylethyl-Isothiocyanate Modulate Akt–mTOR and Cyclin–CDK Signaling in Gemcitabine- and Cisplatin-Resistant Bladder Cancer Cell Lines

doi: 10.3390/ijms231910996

Figure Lengend Snippet: Cell cycle distribution in parental, cisplatin- or gemcitabine-resistant RT112 ( A ) and T24 cells ( B ) following BITC (7.5 µM), AITC (20 µM) or PEITC (7.5 µM) exposure for 24 and/or 72 h. Controls (−) remained untreated. “+” indicates drug treatment. One representative of three separate experiments is shown.

Article Snippet: Bladder cancer cells (50 μL, 1 × 105 cells/mL) were treated with AITC, BITC or PEITC (all from Merck, Darmstadt, Germany) at different concentrations (versus non-treated) and then seeded onto 96-well tissue culture plates.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Allyl-, Butyl- and Phenylethyl-Isothiocyanate Modulate Akt–mTOR and Cyclin–CDK Signaling in Gemcitabine- and Cisplatin-Resistant Bladder Cancer Cell Lines

doi: 10.3390/ijms231910996

Figure Lengend Snippet: Western blot of cell cycle- and mTOR-related proteins from RT112 and T24 lysates of parental and cisplatin/gemcitabine-resistant cells. Tumor cells were pretreated with BITC (7.5 µM), AITC (20 µM) or PEITC (7.5 µM) for 24 and/or 72 h. Controls (Ctrl) remained untreated. Β-actin served as the internal control. One representative from three separate experiments.

Article Snippet: Bladder cancer cells (50 μL, 1 × 105 cells/mL) were treated with AITC, BITC or PEITC (all from Merck, Darmstadt, Germany) at different concentrations (versus non-treated) and then seeded onto 96-well tissue culture plates.

Techniques: Western Blot, Control

Journal: Frontiers in Physiology

Article Title: Heat Perception and Aversive Learning in Honey Bees: Putative Involvement of the Thermal/Chemical Sensor AmHsTRPA

doi: 10.3389/fphys.2015.00316

Figure Lengend Snippet: Effect of topical application of HsTRPA activators on sting extension responses . The bees' mouthparts were stimulated with AITC (allyl isothiocyanate), CA (cinnamaldehyde) or camphor at two concentrations, (A) 1–3 mM or (B) 100–300 mM (all drugs in green). A thermal stimulation (red) or a water control (white) were used as controls. Only 100 mM AITC led to significant SER compared to the water control ( * p < α corr = 0.025).

Article Snippet: While CA and camphor application did not elicit any clear response (Mc Nemar test, Chi 2 < 1.50, NS), AITC induced 32% SER, which was significantly higher than the water control (Mc Nemar test, Chi 2 = 8.10, p < 0.025).

Techniques: Control