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JEOL phase plate airlock system
(a) Diagram of the ZPC microscope illumination <t>system.</t> The <t>phase</t> <t>plate</t> is inserted at the back focal plane of the objective lens. (b) (1) Diagram of the ZPC pole piece showing the positions of specimen, the phase plate and the objective aperture. (2) Photograph of the phase plate <t>airlock</t> system. (c) The phase plate blade can hold two 2-mm phase plate discs, each consisting of 5 × 5 phase plates. Scanning electron microscopy image of a phase plate shows the 0.7-µm hole in the middle.
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SCHOTT schott bottle
(a) Diagram of the ZPC microscope illumination <t>system.</t> The <t>phase</t> <t>plate</t> is inserted at the back focal plane of the objective lens. (b) (1) Diagram of the ZPC pole piece showing the positions of specimen, the phase plate and the objective aperture. (2) Photograph of the phase plate <t>airlock</t> system. (c) The phase plate blade can hold two 2-mm phase plate discs, each consisting of 5 × 5 phase plates. Scanning electron microscopy image of a phase plate shows the 0.7-µm hole in the middle.
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Hitachi Ltd hitachi s
(a) Diagram of the ZPC microscope illumination <t>system.</t> The <t>phase</t> <t>plate</t> is inserted at the back focal plane of the objective lens. (b) (1) Diagram of the ZPC pole piece showing the positions of specimen, the phase plate and the objective aperture. (2) Photograph of the phase plate <t>airlock</t> system. (c) The phase plate blade can hold two 2-mm phase plate discs, each consisting of 5 × 5 phase plates. Scanning electron microscopy image of a phase plate shows the 0.7-µm hole in the middle.
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Image Search Results


(a) Diagram of the ZPC microscope illumination system. The phase plate is inserted at the back focal plane of the objective lens. (b) (1) Diagram of the ZPC pole piece showing the positions of specimen, the phase plate and the objective aperture. (2) Photograph of the phase plate airlock system. (c) The phase plate blade can hold two 2-mm phase plate discs, each consisting of 5 × 5 phase plates. Scanning electron microscopy image of a phase plate shows the 0.7-µm hole in the middle.

Journal: Nature protocols

Article Title: Zernike Phase Contrast Electron Cryo-Tomography Applied to Marine Cyanobacteria Infected with Cyanophages

doi: 10.1038/nprot.2014.176

Figure Lengend Snippet: (a) Diagram of the ZPC microscope illumination system. The phase plate is inserted at the back focal plane of the objective lens. (b) (1) Diagram of the ZPC pole piece showing the positions of specimen, the phase plate and the objective aperture. (2) Photograph of the phase plate airlock system. (c) The phase plate blade can hold two 2-mm phase plate discs, each consisting of 5 × 5 phase plates. Scanning electron microscopy image of a phase plate shows the 0.7-µm hole in the middle.

Article Snippet: Petroff-Hausser counting chamber (EMS, Catalog no. 60512-20) Transmission electron microscope model JEM2200FS (JEOL) Phase plate airlock system (JEOL) Phase plates (Terabase Inc., Japan) Single tilt liquid nitrogen cryo-specimen holder and holder transfer system model 626 (Gatan Inc.) US4000 Charge Coupled Device (CCD) camera model 895 (Gatan Inc.) Vacuum glow discharger (Ernest F. Fullam Inc.) Desktop centrifuge (Eppendorf centrifuge 5417R) FEI Vitrobot Mark III (FEI) Filter paper (Whatman Number 1, Qualitative circles, 55 mm diameter, Catalog no. 1001-055) Quantifoil holey grids (R 2.0/1.0, Cu, 200 mesh, Quantifoil, Germany) Carbon evaporator (Emitech K950) Carbon rods (EMS, Catalog no. 70220-02) Mica sheets (EMS, Catalog no. 71855-05) Software PPCont: phase plate controller provided by JEOL.

Techniques: Microscopy, Electron Microscopy