Journal: PLoS ONE

Article Title: Regulation of Aryl Hydrocarbon Receptor Interacting Protein (AIP) Protein Expression by MiR-34a in Sporadic Somatotropinomas

doi: 10.1371/journal.pone.0117107

Figure Lengend Snippet: TaqMan assays used in real-time qPCR quantification.

Article Snippet: RT-qPCR was performed with the TaqMan system using ready made AIP-probe primer kits (Hs_00610222_m1, Rn_00597273-m1, Life Technologies).

Techniques:

Journal: PLoS ONE

Article Title: Regulation of Aryl Hydrocarbon Receptor Interacting Protein (AIP) Protein Expression by MiR-34a in Sporadic Somatotropinomas

doi: 10.1371/journal.pone.0117107

Figure Lengend Snippet: Demographic, radiological, biochemical and pathological characteristics of the patients with acromegaly.

Article Snippet: RT-qPCR was performed with the TaqMan system using ready made AIP-probe primer kits (Hs_00610222_m1, Rn_00597273-m1, Life Technologies).

Techniques: Biomarker Discovery, Control, Expressing

Journal: PLoS ONE

Article Title: Regulation of Aryl Hydrocarbon Receptor Interacting Protein (AIP) Protein Expression by MiR-34a in Sporadic Somatotropinomas

doi: 10.1371/journal.pone.0117107

Figure Lengend Snippet: A and B—Examples of low AIP expression; C and D: Examples of high AIP expression; E—Normal human pituitary staining with omitting primary antibody (negative control); F—Normal human pituitary staining with AIP (positive control); Scale bar = 1000 μm.

Article Snippet: RT-qPCR was performed with the TaqMan system using ready made AIP-probe primer kits (Hs_00610222_m1, Rn_00597273-m1, Life Technologies).

Techniques: Expressing, Staining, Negative Control, Positive Control

Journal: PLoS ONE

Article Title: Regulation of Aryl Hydrocarbon Receptor Interacting Protein (AIP) Protein Expression by MiR-34a in Sporadic Somatotropinomas

doi: 10.1371/journal.pone.0117107

Figure Lengend Snippet: The 11 miRNAs selected to analyse the expression level in patients with low or high AIP protein expression and the criteria for selection.

Article Snippet: RT-qPCR was performed with the TaqMan system using ready made AIP-probe primer kits (Hs_00610222_m1, Rn_00597273-m1, Life Technologies).

Techniques: Expressing, Selection

Journal: PLoS ONE

Article Title: Regulation of Aryl Hydrocarbon Receptor Interacting Protein (AIP) Protein Expression by MiR-34a in Sporadic Somatotropinomas

doi: 10.1371/journal.pone.0117107

Figure Lengend Snippet: GH3 cells were transfected with plasmids containing pGL3- AIP -3’UTR WT and constructs with mutation in SITE A, SITE B, SITE C and SITE A+C and co-transfected with miR-34a or scrambled control. GH3 cells transfected with empty pGL3 vector and miR-34a or its scrambled control. Data are shown as Firefly/Renilla activity ratios compared to that of the scrambled control transfected cells. Mean ±SEM, *, P <0.05, ***, P <0.001.

Article Snippet: RT-qPCR was performed with the TaqMan system using ready made AIP-probe primer kits (Hs_00610222_m1, Rn_00597273-m1, Life Technologies).

Techniques: Transfection, Construct, Mutagenesis, Control, Plasmid Preparation, Activity Assay

Journal: PLoS ONE

Article Title: Regulation of Aryl Hydrocarbon Receptor Interacting Protein (AIP) Protein Expression by MiR-34a in Sporadic Somatotropinomas

doi: 10.1371/journal.pone.0117107

Figure Lengend Snippet: Effect of miR-34a on endogenous AIP mRNA expression in HEK293 (D) and in GH3 (E) cells 48 hours after transfection measured by RT-qPCR. Data are shown as mean±SEM, **, P <0.01 ***, P <0.001.

Article Snippet: RT-qPCR was performed with the TaqMan system using ready made AIP-probe primer kits (Hs_00610222_m1, Rn_00597273-m1, Life Technologies).

Techniques: Expressing, Transfection, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Phosphoproteomics of CD2 signaling reveals an AMPK-dependent regulation of lytic granule polarization in cytotoxic T cells

doi: 10.1101/795963

Figure Lengend Snippet: (A) Colocalization analysis and (B) immunofluorescence image of LAMP1 and AMPK signals in CTLs. Quantitative measurements were taken for 30 CTLs (n=3 donors). (C) Immunofluorescence images of CTLs electroporated with LAMP1-AIP-mCherry or Tom20-AIP-mCherry constructs and stained with antibodies against perforin (granules). (D) Quantitative analysis of lytic granule polarization in antibody-stimulated mCherry-positive CTLs overexpressing either LAMP1-AIP-mCherry or Tom20-AIP-mCherry (n=3 experiments). (E) Colocalization analysis of immunofluorescence signals in CTLs stained with antibodies against AMPK and perforin (granules) (n=3 experiments). (F) Immunofluorescence image of a CTL stained with antibodies against AMPK, LAMP1 and perforin (granules). (G) CLEM analysis of lytic granules and AMPK-positive compartment in freshly isolated CTLs. N, nucleus; LD, lipid droplet; AMPK, AMPK-positive vesicle; LG, lytic granule. Scale bar, 5 μm. Shown are mean values ± SD; ns, not significant; two-way ANOVA test ****p < 0.0001.

Article Snippet: For overexpression of LAMP1-AIP and Tom20-AIP, CTLs were nucleofected with 2 μg of purified LAMP-mChF-AIP and Tom20-mChF-AIP plasmids (a gift from Takanari Inoue, Addgene plasmids # 61524 and # 61512).

Techniques: Immunofluorescence, Construct, Staining, Isolation

Journal: Heliyon

Article Title: PSO/SDF-1 composite hydrogel promotes osteogenic differentiation of PDLSCs and bone regeneration in periodontitis rats.

doi: 10.1016/j.heliyon.2024.e32686

Figure Lengend Snippet: Fig. 6. Expression of osteogenic genes of PDLSCs by the hydrogels. (A) The mRNA expression level of ALP, RUNX2 and OCN. (B) The protein expression level of ALP, RUNX2, and OCN. Data are expressed as mean ± SD, N = 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 vs Control group.

Article Snippet: Rabbit anti-ALP antibody (1:100, 18176-1-AP, Proteintech), RUNX2 (1:100, ab236639, Abcam), and OCN (1:100, 23418-1-AP, Proteintech) were used to incubated on sections overnight at 4 ◦C.

Techniques: Expressing, Control

Journal: Heliyon

Article Title: PSO/SDF-1 composite hydrogel promotes osteogenic differentiation of PDLSCs and bone regeneration in periodontitis rats.

doi: 10.1016/j.heliyon.2024.e32686

Figure Lengend Snippet: Fig. 8. IHC analysis of different hydrogels. (A) Representative pictures of IHC of ALP. The scale bar is 500 μm and 100 μm. (B) Representative pictures of IHC of RUNX2. The scale bar is 500 μm and 100 μm. (C) Representative pictures of IHC of OCN. The scale bar is 500 μm and 100 μm. (D) IHC quantitative analysis of ALP. (E) IHC quantitative analysis of RUNX2. (F) IHC quantitative analysis of OCN. Data are expressed as mean ± SD, N = 6. ###, P < 0.001; ####, P < 0.0001 vs Model group.

Article Snippet: Rabbit anti-ALP antibody (1:100, 18176-1-AP, Proteintech), RUNX2 (1:100, ab236639, Abcam), and OCN (1:100, 23418-1-AP, Proteintech) were used to incubated on sections overnight at 4 ◦C.

Techniques: