Journal: Cell Reports Methods

Article Title: In situ microwave fixation provides an instantaneous snapshot of the brain metabolome

doi: 10.1016/j.crmeth.2023.100455

Figure Lengend Snippet:

Article Snippet: GLUT3, Rabbit , Alomone , Cat# AGT-023; RRID:AB_2756644.

Techniques:

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: The Hardy–Weinberg Equilibrium for AGT rs699 and AGTR1 rs5186 in the Study Population (P <0.05 Indicates Results are Not Consistent with the Hardy–Weinberg Equilibrium)

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: Baseline Characteristics of the Study Cohort Classified by SNPs and Genotype

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: Associations Between AGT and AGTR1 Polymorphisms and Changes in SBP and DBP at Baseline and at the 5-Year Follow-Up According to Mode of Inheritance

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: Association Between Haplotype Variations in AGT rs699 and AGTR1 rs5186 and SBP and DBP at Baseline and at the 5-Year Follow-Up

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: Odds ratios of hypertension and hypertension with metabolic syndrome depending on haplotype variations of AGT rs699 and AGTR1 rs5186.

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: A network of AGT and AGTR1 gene interactions using GeneMania, ( http://genemania.org ).

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Activation of angiogenin expression in macrophages by lipopolysaccharide via the TLR4/NF-κB pathway in colitis

doi: 10.3724/abbs.2024013

Figure Lengend Snippet: Table 1 Sequences of primers and siRNAs used in this study

Article Snippet: The membrane was blocked with 5% nonfat milk and then incubated with primary antibodies, including anti-ANG antibody (prepared in our own laboratory) and anti-ACTB antibody (#81115-1-RR; Proteintech, Chicago, USA), in TBST (Tris-buffered saline, 0.1% Tween 20) buffer at 4°C overnight.

Techniques: Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Enhanced Cardiorenal Protective Effects of Combining SGLT2 Inhibition, Endothelin Receptor Antagonism and RAS Blockade in Type 2 Diabetic Mice

doi: 10.3390/ijms232112823

Figure Lengend Snippet: Kidney, urine, and serum angiotensinogen and renal renin levels in vehicle-treated db/m mice, vehicle-treated db/db mice, and db/db mice treated with empagliflozin, atrasentan, ramipril, or their combinations. ( A ) Angiotensinogen (AGT) mRNA expression. ( B ) Urine AGT-to-creatinine ratio. ( C ) Serum AGT values. ( D ) Renin mRNA expression. ( E ) Representative kidney sections showing specific renin staining (×400 magnification). Increased juxtaglomerular cell staining (grey arrows) is observed in treatments with ramipril and or ramipril combination with empagliflozin and/or atrasentan. ( F ) Renin stained area in µm 2 . db/m : non-diabetic mice treated with vehicle. db/db : diabetic mice treated with vehicle. db/db EMP : diabetic mice treated with empagliflozin. db/db EMP + RAM : diabetic mice treated with empagliflozin and ramipril. db/db EMP + RAM + ATR : diabetic mice treated with empagliflozin, ramipril, and atrasentan. db/db ATR : diabetic mice treated with atrasentan. db/db ATR + RAM : diabetic mice treated with atrasentan and ramipril. db/db RAM : diabetic mice treated with ramipril. Factorial ANOVA main effect results are displayed next to the graph. P Diabetes : diabetes’ main effect. P EMP : empagliflozin’s main effect. P RAM : ramipril’s main effect. P ATR : atrasentan’s main effect. $ p < 0.05 vehicle-treated db/db mice vs. vehicle-treated db/m mice. * p < 0.05 any treated db/db mice compared with vehicle-treated db/db mice. φ p < 0.05 combinations of empagliflozin or atrasentan with ramipril db/db vs. ramipril db/db.

Article Snippet: Angiotensinogen levels were assessed in urine and serum after treatments using a commercial ELISA kit (Mouse angiotensinogen (aGT) ELISA Kit, CSB-E08566m, Cusabio, Houston, TX, USA) following manufacturer instructions.

Techniques: Expressing, Staining