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  • 94
    Agilent technologies agilent 1100 hplc
    aps1 and pgm leaves accumulate WT ADPG content. (A) <t>HPLC-MS/MS</t> detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an <t>Agilent</t> 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.
    Agilent 1100 Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 3359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies agilent 1100 series
    aps1 and pgm leaves accumulate WT ADPG content. (A) <t>HPLC-MS/MS</t> detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an <t>Agilent</t> 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.
    Agilent 1100 Series, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies agilent 1100 pump
    aps1 and pgm leaves accumulate WT ADPG content. (A) <t>HPLC-MS/MS</t> detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an <t>Agilent</t> 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.
    Agilent 1100 Pump, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies quaternary agilent 1100 series hplc pump
    aps1 and pgm leaves accumulate WT ADPG content. (A) <t>HPLC-MS/MS</t> detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an <t>Agilent</t> 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.
    Quaternary Agilent 1100 Series Hplc Pump, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Agilent technologies agilent 1100 msd
    aps1 and pgm leaves accumulate WT ADPG content. (A) <t>HPLC-MS/MS</t> detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an <t>Agilent</t> 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.
    Agilent 1100 Msd, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies agilent 1100 1200 hplc
    aps1 and pgm leaves accumulate WT ADPG content. (A) <t>HPLC-MS/MS</t> detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an <t>Agilent</t> 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.
    Agilent 1100 1200 Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Agilent technologies agilent model 1100
    aps1 and pgm leaves accumulate WT ADPG content. (A) <t>HPLC-MS/MS</t> detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an <t>Agilent</t> 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.
    Agilent Model 1100, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies agilent 1100 ms g1946
    aps1 and pgm leaves accumulate WT ADPG content. (A) <t>HPLC-MS/MS</t> detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an <t>Agilent</t> 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.
    Agilent 1100 Ms G1946, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies agilent 1100 device
    aps1 and pgm leaves accumulate WT ADPG content. (A) <t>HPLC-MS/MS</t> detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an <t>Agilent</t> 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.
    Agilent 1100 Device, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies agilent 1100 nanopump
    aps1 and pgm leaves accumulate WT ADPG content. (A) <t>HPLC-MS/MS</t> detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an <t>Agilent</t> 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.
    Agilent 1100 Nanopump, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    aps1 and pgm leaves accumulate WT ADPG content. (A) HPLC-MS/MS detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an Agilent 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.

    Journal: PLoS ONE

    Article Title: HPLC-MS/MS Analyses Show That the Near-Starchless aps1 and pgm Leaves Accumulate Wild Type Levels of ADPglucose: Further Evidence for the Occurrence of Important ADPglucose Biosynthetic Pathway(s) Alternative to the pPGI-pPGM-AGP Pathway

    doi: 10.1371/journal.pone.0104997

    Figure Lengend Snippet: aps1 and pgm leaves accumulate WT ADPG content. (A) HPLC-MS/MS detection of ADPG in WT, aps1 and pgm leaves. Upper panels: Total ion chromatograms (TIC) of extracts from the indicated plants in which the selected fragmentation parent ion was 587.8 m/z. Middle panels: Extracted ion chromatograms (EIC) in which the selected ion for fragmentation of the parent ion was 346.1 m/z. Lower panels: Mass spectra (MS2) obtained from fragmentation of parent ion. ADPG was measured using an Agilent 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent) (see Materials and Methods for further details). (B) ADPG content in WT, aps1, pgm and aps1/pgm leaves. Plants were simultaneously grown either in soil or solid MS. Leaves from 4-weeks old WT, aps1, pgm and aps1/pgm plants were simultaneously harvested after 10 h of illumination. ADPG was simultaneouly extracted from leaves of WT, aps1, pgm and aps1/pgm plants and content was simultaneously measured by HPLC-MS/MS as described in Materials and Methods . Note that, consistent with [15] , leaves of aps1, pgm and aps1/pgm plants accumulated WT ADPG content. Values represent the mean ±SD of determinations on three independent samples.

    Article Snippet: ADPG content in leaves of plants cultured on soil was measured in the Research Support Service at the Public University of Navarra using an Agilent 1100 HPLC fitted with a Xbridge C18 column (100×3.0 mm I.D. particle size 3.5 µm) coupled to a MSD-Trap spectrometer (Agilent).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry