Journal: Stem Cells Translational Medicine

Article Title: Extracellular Vesicles From Mesenchymal Umbilical Cord Cells Exert Protection Against Oxidative Stress and Fibrosis in a Rat Model of Bronchopulmonary Dysplasia

doi: 10.1093/stcltm/szad070

Figure Lengend Snippet: Antibody list.

Article Snippet: Goat anti RAGE , AF1145 , BIO-TECHNE.

Techniques:

Journal: Molecular immunology

Article Title: S100A11 regulates nasal epithelial cell remodeling and inflammation in CRSwNPs via the RAGE-mediated AMPK-STAT3 pathway.

doi: 10.1016/j.molimm.2021.09.014

Figure Lengend Snippet: Fig. 5. S100A11 depends on RAGE-mediated can activate AMPK and inhibit STAT3 signaling pathway. A. The phosphorylation levels of AMPK and STAT3 were detected by Western blot after S100A11 was overexpressed. B. The histogram shows the phosphorylation ratio of AMPK and STAT3 (pAMPK/ AMPK and pSTAT3/ STAT3). C. Western blot showed the expression of cleaved-PARP, CCND1, CCNE, Bcl-2, AMPK and STAT3 phosphorylated proteins after treatment of cells with different concentrations of rh-S100A11 protein (0~10000ug/mL) for 24 h. D. Cell immunofluorescence staining detected the expression of STAT3 phosphorylated protein after treatment with 1000ug/mL rh-S100A11 protein for 24 h (bar = 20um). E. The relative mRNA levels of Fas, Stk35, Tsc2 and Socs3 after cells were treated with 1000ug/mL rh-S100A11 protein or transfected for 24 h. F. RAGE protein inhibitors affect the phosphorylation levels of AMPK and STAT3 caused by rhS100A11. The bar graphs show quantification of the results, with each value represents the mean ± SD of three independent experiments. Statistical significance is shown using the Student’s t-test analysis, *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Rabbit polyclonal anti− CCND1 antibody (Cat. No. 60,186-1-Ig, 1:2000 diluted), anti-RAGE antibody (Cat. No. 66,833-1-Ig, 1:2000 diluted) and anti− CCNE antibody (Cat. No. 11554− 1-AP, 1:2000 diluted for WB) were purchased from Proteintech.

Techniques: Phospho-proteomics, Western Blot, Expressing, Immunofluorescence, Staining, Transfection

Journal: Experimental and Therapeutic Medicine

Article Title: Epigallocatechin-3-gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression

doi: 10.3892/etm.2017.4774

Figure Lengend Snippet: EGCG inhibits balloon injury-induced HMGB1 and RAGE expression levels. mRNA expression levels of (A) HMGB1 and (B) RAGE in artery tissues were determined by reverse transcription-quantitative polymerase chain reaction. Protein expression levels of (C) HMGB1 and (D) RAGE in artery tissues were detected by western blotting. β-actin was used as a loading control. The protein bands were quantified by gray scanning. Data are presented as the mean + standard deviation (n=6). *P<0.05, **P<0.01 and ***P<0.001 vs. the sham group; # P<0.05, ## P<0.01 and ### P<0.001 vs. the injury group. EGCG, epigallocatechin-3-gallate; HMGB1, high mobility group box 1; RAGE, receptor of advanced glycation end products.

Article Snippet: Subsequent to blocking with 5% skimmed milk for 1 h at room temperature, the membranes were incubated with primary antibodies against HMGB1 (Boster Biological Technology, Ltd., Wuhan, China; catalogue no. BA4277; 1:400), RAGE (Boster Biological Technology, Ltd.; catalogue no. PB0530; 1:400), nuclear factor (NF)-κB (Boster Biological Technology, Ltd.; catalogue no. BA0610; 1:400) and β-actin (Santa Cruz Biotechnology, Dallas, USA; catalogue no. sc-47778; 1:400), respectively at 4°C overnight.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Control, Standard Deviation

Journal: Oncotarget

Article Title: AGER rs2070600 polymorphism elevates neutrophil-lymphocyte ratio and mortality in metastatic lung adenocarcinoma

doi: 10.18632/oncotarget.21764

Figure Lengend Snippet: Associations of the AGER polymorphisms with the risk of lung adenocarcinoma

Article Snippet: The PCR mixture contained TaqMan SNP Genotyping Assay (C__15867521_20, C___3293837_1_, C___8848033_1_; Life Technologies Corp., Carlsbad, CA, USA), genomic DNA (2.0 g/mL), and TaqMan Fast Universal PCR Master Mix (Life Technologies Corp.).

Techniques: Mutagenesis

Journal: Oncotarget

Article Title: AGER rs2070600 polymorphism elevates neutrophil-lymphocyte ratio and mortality in metastatic lung adenocarcinoma

doi: 10.18632/oncotarget.21764

Figure Lengend Snippet: Box plots showing the range of neutrophil-lymphocyte ratios with/without the AGER rs2070600 minor allele (T) in the patients with metastatic lung adenocarcinoma (n = 96) (A), and exploratory subgroup analysis in patients with EGFR mutation-positive and EGFR mutation-negative lung adenocarcinoma, separately (B). Each box represents the 25 th to 75 th percentiles; solid lines within the boxes indicate the median values; whiskers are the 10 th and 90 th percentiles; and points represent outliers. The p -values were evaluated using Mann–Whitney U -tests.

Article Snippet: The PCR mixture contained TaqMan SNP Genotyping Assay (C__15867521_20, C___3293837_1_, C___8848033_1_; Life Technologies Corp., Carlsbad, CA, USA), genomic DNA (2.0 g/mL), and TaqMan Fast Universal PCR Master Mix (Life Technologies Corp.).

Techniques: Mutagenesis, MANN-WHITNEY

Journal: Oncotarget

Article Title: AGER rs2070600 polymorphism elevates neutrophil-lymphocyte ratio and mortality in metastatic lung adenocarcinoma

doi: 10.18632/oncotarget.21764

Figure Lengend Snippet: Kaplan-Meier analysis showing (A) the survival rate and (B) progression free survival (PFS) with platinum-based chemotherapy in patients with metastatic lung adenocarcinoma, on the presence or absence of the AGER rs2070600 minor allele (T) (n = 96). Solid line indicates patients with the C/C genotype. Dotted line indicates patients with the C/T or T/T genotype. Patients with the AGER rs2070600 minor allele (T) showed significantly poorer survival and shorter PFS compared to those without the minor allele.

Article Snippet: The PCR mixture contained TaqMan SNP Genotyping Assay (C__15867521_20, C___3293837_1_, C___8848033_1_; Life Technologies Corp., Carlsbad, CA, USA), genomic DNA (2.0 g/mL), and TaqMan Fast Universal PCR Master Mix (Life Technologies Corp.).

Techniques: