Journal: Journal of biotechnology

Article Title: Expediting adenovirus titer assays via an algorithmic live-cell imaging technique.

doi: 10.1016/j.jbiotec.2024.09.018

Figure Lengend Snippet: Figure 1. Timelines for the proposed Incucyte assay and the traditional Hexon staining assay. Plates seeded with an HEK 293 cell monolayer were infected with a dilution series created from Ad-GFP virus samples. After a 48-hour incubation period, the cell monolayer was imaged via a Sartorius Incucyte live-cell imaging system, and the FRs corresponding to infected cells were counted using the Incucyte’s segmentation algorithms. The same plates were subjected to the Hexon staining assay, imaged, and the stained regions were counted by the operator.

Article Snippet: [27] I. Essen Bioscience, “IncuCyte Live-Cell Analysis Systems User Manual.” Sartorius, Inc, 2022.

Techniques: Staining, Infection, Virus, Incubation, Live Cell Imaging

Journal: Journal of biotechnology

Article Title: Expediting adenovirus titer assays via an algorithmic live-cell imaging technique.

doi: 10.1016/j.jbiotec.2024.09.018

Figure Lengend Snippet: Figure 4. FR counts for the same image under different edge sensitivities and minimum mean intensities. FRs identified by the Incucyte segmentation algorithm are outlined in pink. The total number of identified FRs in each image is shown in the attached black box. The yellow and cyan squares are used to outline specific FRs across all parameter conditions. The above image was acquired using the “Autoscale Well” setting and the 24-well plate used in R3. It depicts a cellular monolayer infected with a viral solution dilution factor of 7 (see Supplemental Information, Section 2, for more information regarding the imaging layout).

Article Snippet: [27] I. Essen Bioscience, “IncuCyte Live-Cell Analysis Systems User Manual.” Sartorius, Inc, 2022.

Techniques: Infection, Imaging

Journal: Journal of biotechnology

Article Title: Expediting adenovirus titer assays via an algorithmic live-cell imaging technique.

doi: 10.1016/j.jbiotec.2024.09.018

Figure Lengend Snippet: Figure 7. Comparison of infectious titer estimates attained via Incucyte and Hexon staining for various samples (R1 to R6).

Article Snippet: [27] I. Essen Bioscience, “IncuCyte Live-Cell Analysis Systems User Manual.” Sartorius, Inc, 2022.

Techniques: Comparison, Staining

Journal: Journal of biotechnology

Article Title: Expediting adenovirus titer assays via an algorithmic live-cell imaging technique.

doi: 10.1016/j.jbiotec.2024.09.018

Figure Lengend Snippet: Figure 8. FR counts obtained via Incucyte analysis for the dilution series plated on 24-well and 96-well plates for R5. The 96-well data includes samples from dilution factors 4 to 9, while the 24-well data includes samples from dilution factors 6 to 9, enabling a comparison of half-dilution factors from the two datasets. The FR count in the 96-well plate plateaus from dilution factors 4-6, and the normalized standard deviation increases as the count number falls below 200. The count range for the 24-well plate, identified as 300-6500 in prior 24-well runs, is shown in red at the bottom right graph. Both plates were infected in accordance with the plate map depicted in Figure S1 of the Supplemental Information.

Article Snippet: [27] I. Essen Bioscience, “IncuCyte Live-Cell Analysis Systems User Manual.” Sartorius, Inc, 2022.

Techniques: Comparison, Standard Deviation, Infection

Journal: Scientific Reports

Article Title: Effects of releasing ankle joint during electrically evoked cycling in persons with motor complete spinal cord injury

doi: 10.1038/s41598-024-56955-w

Figure Lengend Snippet: Two stimulation modes of FES-evoked cycling were used in this study; ( a ) QH stimulation; ( b ) QHT stimulation; and ( c ) stimulation angle. Image adapted from the software 3D Anatomy Learning (Version 3.9, Education Mobile) (open-source project).

Article Snippet: The pedal power output (PO) and the hip, knee, and ankle joints kinematics of each trial set were recorded at 120 Hz, displayed in real-time using software (Tekscan Incorporated, Boston, Massachusetts) and 3D motion analysis systems (Qualisys AB, Gothenburg, Sweden, and Vicon, Oxford, UK) to store data into a PC for offline analysis.

Techniques: Software