Journal: Development, Growth & Differentiation

Article Title: BMP ‐Dependent Modulation of ROS Generation and Scavenging Controls Interdigital Cell Death

doi: 10.1111/dgd.70034

Figure Lengend Snippet: BMP signaling promotes ROS production in the interdigital regions. (a) Cell death (LysoTracker, green) and ROS (DHE, red) were detected in the hindlimbs of chicken embryos 10–17 h after implantation of heparin‐acrylic beads (asterisks) soaked with PBS ( n = 5) or BMP2 ( n = 7) at HH28‐29. Note that cell death and ROS signals increased around BMP2‐soaked beads (arrowheads). (b) Quantification of LysoTracker‐ or DHE‐positive puncta in ID3 of the left and right hindlimbs indicated in (a). Each pair of symbols represents an individual embryo. Statistical analysis was performed using the Wilcoxon matched‐pairs signed‐rank test, and q values are reported. (c) Cell death (LysoTracker, green) and ROS (DHE, red) signals were detected 13–17 h after implantation of Affi‐Gel Blue Gel beads (asterisks) soaked with PBS ( n = 8) or Noggin ( n = 9) at HH29‐30. Note that cell death and ROS signals were diminished around Noggin‐soaked beads (bracket). (d) Quantification of LysoTracker‐ or DHE‐positive puncta in ID3 of the left and right limbs shown in (c). Each pair of symbols represents an individual embryo. Statistical analysis was performed using the Wilcoxon matched‐pairs signed‐rank test, and q values are reported. Scale bars, 500 μm. ID3, interdigital region 3.

Article Snippet: CM Affi‐Gel Blue Gel beads (153–7304, BioRad, California) were washed, dried, and soaked in Noggin or PBS.

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