Journal: Archives of Gynecology and Obstetrics

Article Title: Glycosylated fibronectin as a first trimester marker for gestational diabetes

doi: 10.1007/s00404-020-05670-8

Figure Lengend Snippet: a Glycosylated fibronectin concentrations in BMI subgroups of 20–25 and 30–35 kg/m 2 in the study and the control group. b Effect of smoking on concentrations of glycosylated fibronectin in the control and GDM groups. c Multiple of median (MoM) values for fibronectin and glycosylated fibronectin between control and GDM groups. d Multiple of median (MoM) values for adiponectin and glycosylated adiponectin between control and GDM groups

Article Snippet: Calibrators for both assays were made from recombinant human adiponectin (1065-AF, R&D Systems, Abingdon, UK).

Techniques: Control

Journal: Nature communications

Article Title: A mouse model for a partially inactive obesity-associated human MC3R variant.

doi: 10.1038/ncomms10522

Figure Lengend Snippet: Figure 8 | Maintained adipose tissue function in MC3RhDM/hDM mice. Gonadal fat was isolated from fasted female mice fed a high-fat diet for (a) quantitative real-time PCR (MC3RhWT/hWT n ¼ 5; MC3RhDM/hDM n ¼ 8/group), (b–d) western blotting analysis (MC3RhWT/hWT n ¼ 5; MC3RhDM/hDM n ¼ 4), and (e–l) FACS analysis (MC3RhWT/hWT n ¼ 4; MC3RhDM/hDM n ¼ 5) in MC3RhWT/hWT (open bars) and MC3RhDM/hDM (closed bars) mice. (a) The expression levels of genes related to macrophage infiltration, inflammation and adipose tissue metabolism were normalized for b-actin expression. Western protein expression results for (b) peroxisome proliferator-activated receptor gamma (PPARg), (c) adiponectin and (d) phosphospecific 50-adenosine monophosphate- activated protein kinase (p-AMPK), divided by glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression and normalized relative to average for MC3RhWT/hWT. The stromal vascular fraction was isolated from gonadal fat (B2 g) from MC3RhWT/hWTand MC3RhDM/hDMmice. For detecting macrophages by flow cytometry, a gating strategy was used to enrich samples from MC3RhWT/hWTand MC3RhDM/hDMmice for adipose tissue macrophages by selecting cells in the gate 1 area (e,g). The gate 1 area was stained with a F4/80 antibody (f and h) and the dot plots depict forward scatter (FSC) and side scatter (SSC) (left). For detecting neutrophils, whole cells (non-gated) were stained with CD11b and Gr-1 (i,j). Bar graphs show the average values±s.e.m. for percentage of cells in the stromal vascular fraction that were (k) macrophages and (l) neutrophils. *Po0.05 MC3RhDM/hDMversus MC3RhWT/hWT. (m,n) Haematoxylin and eosin stained sections showed no apparent differences in adipocyte morphology between groups. Groups were compared by Student’s t-tests (two-tailed) (a–d and k–i). Scale bar, 100 mm. Data are represented as mean±s.e.m. for a-d, k, and l. FACS, fluorescence-activated cell sorting

Article Snippet: Other metabolites or hormones were measured using the indicated kits; Insulin (SRI-13K, Linco Research, St Charles, MO), corticosterone (ADI-900-097; Enzo Life science, Farmingdale, NY), IGF-1 (22-IGF-R21, ALPCO, Salem, NH), Adiponectin (DRP 300 for human plasma samples obtained by venipuncture and MRP300 for mouse serum, R&D SYSTEMS, Minneapolis, MN), Leptin (MOB00, R&D SYSTEMS), triglycerides (337-B; Sigma), and non-esterified fatty acids (13831175; Roche Molecular Biochemicals, Indianapolis, IN).

Techniques: Isolation, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Cytometry, Staining, Two Tailed Test, FACS