Journal: International Journal of Molecular Sciences

Article Title: Adipose-Derived Stem Cells Preincubated with Green Tea EGCG Enhance Pancreatic Tissue Regeneration in Rats with Type 1 Diabetes through ROS/Sirt1 Signaling Regulation

doi: 10.3390/ijms23063165

Figure Lengend Snippet: Characterization of adipose-derived stem cells. ( a ) Surface markers, ( b ) adipogenesis differentiation and ( c ) self-renewal markers.

Article Snippet: The determination of the differentiation capability of stem cells was performed using the mesenchymal adipogenesis kit (Merck KGaA, Darmstadt, Germany) based on the manufacturer’s instructions.

Techniques: Derivative Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: GC-Rich Extracellular DNA Induces Oxidative Stress, Double-Strand DNA Breaks, and DNA Damage Response in Human Adipose-Derived Mesenchymal Stem Cells

doi: 10.1155/2015/782123

Figure Lengend Snippet: GC-DNA induces adipogenic differentiation of haMSCs. HaMSCs were grown to subconfluency (~80%) and then DNA samples were added. Cells were cultivated in the presence of DNA samples (50 ng/mL) at 37°C for 14 days in AmnioMax Basal Medium with AmnioMax Supplement C100 (Gibco). In a week the culture medium with DNA samples was refreshed. (a) Changes in the morphology of the cultured cells (100x). Cells were fixed with isopropanol and stained with crystal violet. Control (−) DNA samples were not added to the medium; control (+) cells were cultured in the medium reliably inducing adipogenesis (StemCell Technologies Inc.). (b) Adipogenic differentiation of haMSCs was identified by fixing the cells with 4% PFA and staining with 0.3% Oil Red O solution and with CytoGreen (20x). (c) Relative levels of RNA for PPARG2 and LPL. The cells were fixed after 7 days of incubation. (d) Detection of FABP4 protein level in haMSCs exposed to DNA samples. Cells were fixed with 3% PFA, treated with 0.1% Triton X-100 and stained with anti-FABP4 (FITC) antibodies. (A) FACS analysis showing the stained cellular fractions (SSC-FL1 (FABP4) diagram). Gate R encircles the fraction of haMSCs that express large amounts of protein FABP4; (B) the distribution of fluorescence intensities of the cells stained with anti-FABP4 (FITC) antibodies; (C) the proportion of cells with large amounts of protein FABP4 (gate R). Horizontal bars reflect relative expression levels in the control cells. Data points were averaged and represented as mean ± SD for three biological replicates. Asterisk ( * ) depicts the differences between exposed cells and control cells that were statistically significant by the Mann-Whitney U test ( P < 0.05).

Article Snippet: Control (−) DNA samples were not added to the medium; control (+) – cells were cultured in the medium reliably inducing adipogenesis (“StemCell Technologies Inc.”).

Techniques: Cell Culture, Staining, Control, Incubation, Fluorescence, Expressing, MANN-WHITNEY