adenylation Search Results


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  • 95
    Millipore adenosine monophosphate
    Tandem MS of molecular ions from cell supernatants and authentic compounds. (A) adenosine <t>monophosphate</t> (AMP), (B) glutathione (GSH), (C) spermine, and (D) nicotinamide adenine dinucleotide (NAD + ).
    Adenosine Monophosphate, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    New England Biolabs dna adenylation kit
    Tandem MS of molecular ions from cell supernatants and authentic compounds. (A) adenosine <t>monophosphate</t> (AMP), (B) glutathione (GSH), (C) spermine, and (D) nicotinamide adenine dinucleotide (NAD + ).
    Dna Adenylation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Bioo Scientific adenylation kit
    Tandem MS of molecular ions from cell supernatants and authentic compounds. (A) adenosine <t>monophosphate</t> (AMP), (B) glutathione (GSH), (C) spermine, and (D) nicotinamide adenine dinucleotide (NAD + ).
    Adenylation Kit, supplied by Bioo Scientific, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Eurofins complete adenylation
    Tandem MS of molecular ions from cell supernatants and authentic compounds. (A) adenosine <t>monophosphate</t> (AMP), (B) glutathione (GSH), (C) spermine, and (D) nicotinamide adenine dinucleotide (NAD + ).
    Complete Adenylation, supplied by Eurofins, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Abcam adenylate cyclase
    A water soluble fraction of Smilax china L. leaf ethanol extract (wsSCLE) stimulates protein kinase A (PKA) signaling. (A) The expression of <t>adenylate</t> cyclase (AC) and PKA by wsSCLE treatment. Mature 3T3-L1 adipocytes were incubated with wsSCLE (0.1 and 0.25 mg/mL) for 24 h. The β-adrenergic receptors were induced (by Iso, an agonist) and inhibited (by Pro, an antagonist). (B) The induction of the phosphorylation (p) of PKA substrates (PKAs) by wsSCLE treatment. H89 treatment (10 µM) decreased wsSCLE-induced pPKAs. Data are expressed as mean ± SD of triplicate experiments, ## P
    Adenylate Cyclase, supplied by Abcam, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Santa Cruz Biotechnology adenylate cyclase
    Conditional loss of Cilia in the adult hippocampus and cortex. ( A ) Immunofluorescence for the neuronal cilia marker <t>adenylate</t> cyclase <t>III</t> (red) in brain sections from the following regions of the brain: Ob, Ctx, and regions of the hippocampus the pyramidal layers CA1, CA3, and granule layer of the dentate gyrus (DG). Ift88 flox/flox control samples (top row) are compared to Ift88 Δ/Δ ; Emx1-cre (bottom row). Hoechst nuclear stain is in blue. Scale bar is 50 µm. ( B ) Graph indicating the amount of cilia counted in several regions of the brain in control ( Ift88 flox/flox , N = 3) and mutants ( Ift88 Δ/Δ N = 4). Statistically significant differences are indicated: Student's t-test, * p
    Adenylate Cyclase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc adenylation
    A schematic representation of the sample preparation workflow. The processes of the TruSeq™ RNA Sample Preparation v2 low sample (LS) protocol (Illumina) performed manually and adopted for automated use with the SX-8G IP-Star® Compact System are illustrated. The automated protocol minimizes the hands-on time required for the error-prone manual steps of RNA-seq library synthesis, <t>adenylation,</t> and adaptor ligation including all related clean up steps and allows experimental multitasking for the researcher in task.
    Adenylation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Bioo Scientific air adenylated linker a
    A schematic representation of the sample preparation workflow. The processes of the TruSeq™ RNA Sample Preparation v2 low sample (LS) protocol (Illumina) performed manually and adopted for automated use with the SX-8G IP-Star® Compact System are illustrated. The automated protocol minimizes the hands-on time required for the error-prone manual steps of RNA-seq library synthesis, <t>adenylation,</t> and adaptor ligation including all related clean up steps and allows experimental multitasking for the researcher in task.
    Air Adenylated Linker A, supplied by Bioo Scientific, used in various techniques. Bioz Stars score: 82/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Promega adenylate tail
    A schematic representation of the sample preparation workflow. The processes of the TruSeq™ RNA Sample Preparation v2 low sample (LS) protocol (Illumina) performed manually and adopted for automated use with the SX-8G IP-Star® Compact System are illustrated. The automated protocol minimizes the hands-on time required for the error-prone manual steps of RNA-seq library synthesis, <t>adenylation,</t> and adaptor ligation including all related clean up steps and allows experimental multitasking for the researcher in task.
    Adenylate Tail, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Mimetics ub adenylate mimetics
    A schematic representation of the sample preparation workflow. The processes of the TruSeq™ RNA Sample Preparation v2 low sample (LS) protocol (Illumina) performed manually and adopted for automated use with the SX-8G IP-Star® Compact System are illustrated. The automated protocol minimizes the hands-on time required for the error-prone manual steps of RNA-seq library synthesis, <t>adenylation,</t> and adaptor ligation including all related clean up steps and allows experimental multitasking for the researcher in task.
    Ub Adenylate Mimetics, supplied by Mimetics, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore adenyl cyclase
    A schematic representation of the sample preparation workflow. The processes of the TruSeq™ RNA Sample Preparation v2 low sample (LS) protocol (Illumina) performed manually and adopted for automated use with the SX-8G IP-Star® Compact System are illustrated. The automated protocol minimizes the hands-on time required for the error-prone manual steps of RNA-seq library synthesis, <t>adenylation,</t> and adaptor ligation including all related clean up steps and allows experimental multitasking for the researcher in task.
    Adenyl Cyclase, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore adenylate kinase
    A schematic representation of the sample preparation workflow. The processes of the TruSeq™ RNA Sample Preparation v2 low sample (LS) protocol (Illumina) performed manually and adopted for automated use with the SX-8G IP-Star® Compact System are illustrated. The automated protocol minimizes the hands-on time required for the error-prone manual steps of RNA-seq library synthesis, <t>adenylation,</t> and adaptor ligation including all related clean up steps and allows experimental multitasking for the researcher in task.
    Adenylate Kinase, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Enzymatics adenylation
    A schematic representation of the sample preparation workflow. The processes of the TruSeq™ RNA Sample Preparation v2 low sample (LS) protocol (Illumina) performed manually and adopted for automated use with the SX-8G IP-Star® Compact System are illustrated. The automated protocol minimizes the hands-on time required for the error-prone manual steps of RNA-seq library synthesis, <t>adenylation,</t> and adaptor ligation including all related clean up steps and allows experimental multitasking for the researcher in task.
    Adenylation, supplied by Enzymatics, used in various techniques. Bioz Stars score: 87/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Illumina Inc pre adenylated linker ra3
    A schematic representation of the sample preparation workflow. The processes of the TruSeq™ RNA Sample Preparation v2 low sample (LS) protocol (Illumina) performed manually and adopted for automated use with the SX-8G IP-Star® Compact System are illustrated. The automated protocol minimizes the hands-on time required for the error-prone manual steps of RNA-seq library synthesis, <t>adenylation,</t> and adaptor ligation including all related clean up steps and allows experimental multitasking for the researcher in task.
    Pre Adenylated Linker Ra3, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Agilent technologies viral poly adenylated rnas
    A schematic representation of the sample preparation workflow. The processes of the TruSeq™ RNA Sample Preparation v2 low sample (LS) protocol (Illumina) performed manually and adopted for automated use with the SX-8G IP-Star® Compact System are illustrated. The automated protocol minimizes the hands-on time required for the error-prone manual steps of RNA-seq library synthesis, <t>adenylation,</t> and adaptor ligation including all related clean up steps and allows experimental multitasking for the researcher in task.
    Viral Poly Adenylated Rnas, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 81/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Santa Cruz Biotechnology adenylate cyclases
    RPS23RG family proteins interact with <t>adenylate</t> cyclases, activate PKA and reduce GSK-3 activity, Aβ level and tau phosphorylation. ( A ) RPS23RG family proteins interact with adenylate cyclase 8 (AC8). N2a cells were transfected with RPS23RG1, RPS23RG2 or RPS23RG2CΔ37, all of which are Myc-tagged, together with AC8. Co-immunoprecipitation assays in these cells showed that an adenylate cyclase (AC) antibody pulled down RPS23RG1, RPS23RG2 and RPS23RG2CΔ37 as the Myc antibody 9E10 did (upper panel), and that the Myc antibody pulled down AC as the AC antibody did (lower panel). However, mouse IgG (mIgG) and rabbit IgG (rIgG) did not pull down RPS23RG family proteins or AC. *Non-specific bands. Note that rIgG pulled down a non-specific band that is localized slightly higher than the AC band. ( B ) RPS23RG family proteins modulate the activities of PKA and GSK-3, APP processing/Aβ level and tau phosphorylation. Overexpression of RPS23RG1, RPS23RG2 and RPS23RG2CΔ37 in human HeLa cells stably expressing human APP Swedish mutation increased the levels of phosphorylated CREB indicative of upregulated PKA activity, phosphorylated GSK-3α/β indicative of reduced GSK-3 activity, sAPPα and APP CTFs, and decreased Aβ level and tau phosphorylation. p: phosphorylated forms. In some experiments, transfected cells were subjected to biotinylation and affinity precipitation to detect cell surface levels of transfected proteins. In some experiments, protein levels were quantified by densitometry and normalized to those of controls for comparison (set as one arbitrary unit). Error bars indicate SEM. # P
    Adenylate Cyclases, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 76/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore adenylate myo kinase
    RPS23RG family proteins interact with <t>adenylate</t> cyclases, activate PKA and reduce GSK-3 activity, Aβ level and tau phosphorylation. ( A ) RPS23RG family proteins interact with adenylate cyclase 8 (AC8). N2a cells were transfected with RPS23RG1, RPS23RG2 or RPS23RG2CΔ37, all of which are Myc-tagged, together with AC8. Co-immunoprecipitation assays in these cells showed that an adenylate cyclase (AC) antibody pulled down RPS23RG1, RPS23RG2 and RPS23RG2CΔ37 as the Myc antibody 9E10 did (upper panel), and that the Myc antibody pulled down AC as the AC antibody did (lower panel). However, mouse IgG (mIgG) and rabbit IgG (rIgG) did not pull down RPS23RG family proteins or AC. *Non-specific bands. Note that rIgG pulled down a non-specific band that is localized slightly higher than the AC band. ( B ) RPS23RG family proteins modulate the activities of PKA and GSK-3, APP processing/Aβ level and tau phosphorylation. Overexpression of RPS23RG1, RPS23RG2 and RPS23RG2CΔ37 in human HeLa cells stably expressing human APP Swedish mutation increased the levels of phosphorylated CREB indicative of upregulated PKA activity, phosphorylated GSK-3α/β indicative of reduced GSK-3 activity, sAPPα and APP CTFs, and decreased Aβ level and tau phosphorylation. p: phosphorylated forms. In some experiments, transfected cells were subjected to biotinylation and affinity precipitation to detect cell surface levels of transfected proteins. In some experiments, protein levels were quantified by densitometry and normalized to those of controls for comparison (set as one arbitrary unit). Error bars indicate SEM. # P
    Adenylate Myo Kinase, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Lonza adenylate kinase release
    DIDS abolishes pathology-induced membrane degradation. Stress-induced vital dye uptake and <t>adenylate</t> kinase release are prevented by DIDS treatment. ( A ) Summary of the effect of DIDS on stress-induced propidium iodide (PI) uptake. ( B C ) Dose-response relationship of IS- (B) or STS-mediated (C) PI uptake vs. [DIDS]. ( D ) Confocal fluorescence images of PI fluorescence (red) from neurons treated as indicated. Nuclei were stained with DAPI (blue) for co-localization analysis. Arrows indicate representative neurons that have taken up PI. ( E ) Summary of the effect of DIDS on stress-induced trypan blue (TB) uptake. ( F ) Summary of the effect of DIDS on stress-induced adenylate kinase (AK) release. ( G ) Summary of plasma membrane widths measured from TEM analysis. Data are mean ±SEM from 3-10 separate 24-hr experiments. Asterisks (*) indicate significant difference from normoxic controls; black bars indicate significance between connected treatments ( p
    Adenylate Kinase Release, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    TriLink pre adenylated linker l3
    DIDS abolishes pathology-induced membrane degradation. Stress-induced vital dye uptake and <t>adenylate</t> kinase release are prevented by DIDS treatment. ( A ) Summary of the effect of DIDS on stress-induced propidium iodide (PI) uptake. ( B C ) Dose-response relationship of IS- (B) or STS-mediated (C) PI uptake vs. [DIDS]. ( D ) Confocal fluorescence images of PI fluorescence (red) from neurons treated as indicated. Nuclei were stained with DAPI (blue) for co-localization analysis. Arrows indicate representative neurons that have taken up PI. ( E ) Summary of the effect of DIDS on stress-induced trypan blue (TB) uptake. ( F ) Summary of the effect of DIDS on stress-induced adenylate kinase (AK) release. ( G ) Summary of plasma membrane widths measured from TEM analysis. Data are mean ±SEM from 3-10 separate 24-hr experiments. Asterisks (*) indicate significant difference from normoxic controls; black bars indicate significance between connected treatments ( p
    Pre Adenylated Linker L3, supplied by TriLink, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Lonza adenylate kinase reagent
    <t>Adenylate</t> kinase activities of MRSA cultures treated with different concentrations of azalomycin F 5a . MRSA ATCC 33592 cultures, respectively, treated with 1.0, 2.0, 4.0, and 8.0 μ g/mL of azalomycin F 5a , were incubated at 37°C for 6 h, and their luminescence ( n = 3) was recorded using a SpectraMax M5 microplate reader. ∗ indicates a significant difference between blank control and azalomycin F 5a group with specific concentration ( P ≤ 0.05).
    Adenylate Kinase Reagent, supplied by Lonza, used in various techniques. Bioz Stars score: 81/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore adenyl cyclase activator
    <t>Adenylate</t> kinase activities of MRSA cultures treated with different concentrations of azalomycin F 5a . MRSA ATCC 33592 cultures, respectively, treated with 1.0, 2.0, 4.0, and 8.0 μ g/mL of azalomycin F 5a , were incubated at 37°C for 6 h, and their luminescence ( n = 3) was recorded using a SpectraMax M5 microplate reader. ∗ indicates a significant difference between blank control and azalomycin F 5a group with specific concentration ( P ≤ 0.05).
    Adenyl Cyclase Activator, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Trinity Biotech dna adenylation kit
    <t>Adenylate</t> kinase activities of MRSA cultures treated with different concentrations of azalomycin F 5a . MRSA ATCC 33592 cultures, respectively, treated with 1.0, 2.0, 4.0, and 8.0 μ g/mL of azalomycin F 5a , were incubated at 37°C for 6 h, and their luminescence ( n = 3) was recorded using a SpectraMax M5 microplate reader. ∗ indicates a significant difference between blank control and azalomycin F 5a group with specific concentration ( P ≤ 0.05).
    Dna Adenylation Kit, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    75
    Cambrex adenylate nucleotide ratio assay
    <t>Adenylate</t> kinase activities of MRSA cultures treated with different concentrations of azalomycin F 5a . MRSA ATCC 33592 cultures, respectively, treated with 1.0, 2.0, 4.0, and 8.0 μ g/mL of azalomycin F 5a , were incubated at 37°C for 6 h, and their luminescence ( n = 3) was recorded using a SpectraMax M5 microplate reader. ∗ indicates a significant difference between blank control and azalomycin F 5a group with specific concentration ( P ≤ 0.05).
    Adenylate Nucleotide Ratio Assay, supplied by Cambrex, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher full adenylation
    <t>Adenylate</t> kinase activities of MRSA cultures treated with different concentrations of azalomycin F 5a . MRSA ATCC 33592 cultures, respectively, treated with 1.0, 2.0, 4.0, and 8.0 μ g/mL of azalomycin F 5a , were incubated at 37°C for 6 h, and their luminescence ( n = 3) was recorded using a SpectraMax M5 microplate reader. ∗ indicates a significant difference between blank control and azalomycin F 5a group with specific concentration ( P ≤ 0.05).
    Full Adenylation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Thermo Fisher poly adenylated
    <t>Adenylate</t> kinase activities of MRSA cultures treated with different concentrations of azalomycin F 5a . MRSA ATCC 33592 cultures, respectively, treated with 1.0, 2.0, 4.0, and 8.0 μ g/mL of azalomycin F 5a , were incubated at 37°C for 6 h, and their luminescence ( n = 3) was recorded using a SpectraMax M5 microplate reader. ∗ indicates a significant difference between blank control and azalomycin F 5a group with specific concentration ( P ≤ 0.05).
    Poly Adenylated, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Enzo Biochem adenylate cyclases
    <t>Adenylate</t> kinase activities of MRSA cultures treated with different concentrations of azalomycin F 5a . MRSA ATCC 33592 cultures, respectively, treated with 1.0, 2.0, 4.0, and 8.0 μ g/mL of azalomycin F 5a , were incubated at 37°C for 6 h, and their luminescence ( n = 3) was recorded using a SpectraMax M5 microplate reader. ∗ indicates a significant difference between blank control and azalomycin F 5a group with specific concentration ( P ≤ 0.05).
    Adenylate Cyclases, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore pacap
    Epac2 is involved in <t>PACAP-induced</t> astrocytogenesis. (A) GFAP immunolabeling of neural precursor cells (NPCs) at day 0 (DAT0). NPCs from neither wild-type (WT) nor Epac2-knockout (KO) mice expressed GFAP at DAT0. (B) WT NPCs remarkably differentiated into astrocytes when cultured in <t>bFGF-free</t> media for 4 days (at DAT4), but KO NPCs showed much lower GFAP immunoreactivity than WT NPCs. (C) CNTF (50 ng/ml) induced differentiation of NPCs of both genotypes into astrocytes. (D) WT NPCs incubated in PACAP (100 nM)-treated media for 4 days expressed GFAP, but KO NPCs did not display astrocytic differentiation. (E) Ratio of GFAP-immunopositive cell number to total NPCs number. KO NPCs did not show GFAP-immunopositive astrocytes by DAT4 in bFGF-free media ( # P = 0.0001, independent-samples t -test, trial number = 8). Treatment with either CNTF or PACAP increased differentiation of WT NPCs into astrocytes compared to WT NPCs without treatment at DAT4 (*P = 0.003 for CNTF, *P = 0.002 for PACAP, independent-samples t -test, trial number in each experiment = 8), but KO NPCs did not induce astrocytic differentiation after PACAP treatment contrast to CNTF treatment ( ## P = 0.0002, independent-samples t -test, trial number = 8). (F) GFAP intensity of immunopositive cells. KO NPCs showed much lower intensity in GFAP immunoreactivity compared to WT NPCs at DAT4 in bFGF-free media ( # P = 0.0066, independent-samples t -test). Treatment with either CNTF or PACAP increased GFAP intensity in WT NPCs compared to WT NPCs without treatment at DAT4 (*P = 0.024 for CNTF, *P = 0.007 for PACAP, independent-samples t -test), but contrast to CNTF, KO NPCs did not increase GFAP immunoreactivity even after PACAP treatment compared to WT NPCs ( ## P = 0.00004, independent-samples t -test). Scale bar = 200 μm.
    Pacap, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs adenylation kit
    Epac2 is involved in <t>PACAP-induced</t> astrocytogenesis. (A) GFAP immunolabeling of neural precursor cells (NPCs) at day 0 (DAT0). NPCs from neither wild-type (WT) nor Epac2-knockout (KO) mice expressed GFAP at DAT0. (B) WT NPCs remarkably differentiated into astrocytes when cultured in <t>bFGF-free</t> media for 4 days (at DAT4), but KO NPCs showed much lower GFAP immunoreactivity than WT NPCs. (C) CNTF (50 ng/ml) induced differentiation of NPCs of both genotypes into astrocytes. (D) WT NPCs incubated in PACAP (100 nM)-treated media for 4 days expressed GFAP, but KO NPCs did not display astrocytic differentiation. (E) Ratio of GFAP-immunopositive cell number to total NPCs number. KO NPCs did not show GFAP-immunopositive astrocytes by DAT4 in bFGF-free media ( # P = 0.0001, independent-samples t -test, trial number = 8). Treatment with either CNTF or PACAP increased differentiation of WT NPCs into astrocytes compared to WT NPCs without treatment at DAT4 (*P = 0.003 for CNTF, *P = 0.002 for PACAP, independent-samples t -test, trial number in each experiment = 8), but KO NPCs did not induce astrocytic differentiation after PACAP treatment contrast to CNTF treatment ( ## P = 0.0002, independent-samples t -test, trial number = 8). (F) GFAP intensity of immunopositive cells. KO NPCs showed much lower intensity in GFAP immunoreactivity compared to WT NPCs at DAT4 in bFGF-free media ( # P = 0.0066, independent-samples t -test). Treatment with either CNTF or PACAP increased GFAP intensity in WT NPCs compared to WT NPCs without treatment at DAT4 (*P = 0.024 for CNTF, *P = 0.007 for PACAP, independent-samples t -test), but contrast to CNTF, KO NPCs did not increase GFAP immunoreactivity even after PACAP treatment compared to WT NPCs ( ## P = 0.00004, independent-samples t -test). Scale bar = 200 μm.
    Adenylation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cytoplasmic Polyadenylation Element Binding Protein 1 Human Recombinant 20 ug
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    Illumina Inc pre adenylated
    Epac2 is involved in <t>PACAP-induced</t> astrocytogenesis. (A) GFAP immunolabeling of neural precursor cells (NPCs) at day 0 (DAT0). NPCs from neither wild-type (WT) nor Epac2-knockout (KO) mice expressed GFAP at DAT0. (B) WT NPCs remarkably differentiated into astrocytes when cultured in <t>bFGF-free</t> media for 4 days (at DAT4), but KO NPCs showed much lower GFAP immunoreactivity than WT NPCs. (C) CNTF (50 ng/ml) induced differentiation of NPCs of both genotypes into astrocytes. (D) WT NPCs incubated in PACAP (100 nM)-treated media for 4 days expressed GFAP, but KO NPCs did not display astrocytic differentiation. (E) Ratio of GFAP-immunopositive cell number to total NPCs number. KO NPCs did not show GFAP-immunopositive astrocytes by DAT4 in bFGF-free media ( # P = 0.0001, independent-samples t -test, trial number = 8). Treatment with either CNTF or PACAP increased differentiation of WT NPCs into astrocytes compared to WT NPCs without treatment at DAT4 (*P = 0.003 for CNTF, *P = 0.002 for PACAP, independent-samples t -test, trial number in each experiment = 8), but KO NPCs did not induce astrocytic differentiation after PACAP treatment contrast to CNTF treatment ( ## P = 0.0002, independent-samples t -test, trial number = 8). (F) GFAP intensity of immunopositive cells. KO NPCs showed much lower intensity in GFAP immunoreactivity compared to WT NPCs at DAT4 in bFGF-free media ( # P = 0.0066, independent-samples t -test). Treatment with either CNTF or PACAP increased GFAP intensity in WT NPCs compared to WT NPCs without treatment at DAT4 (*P = 0.024 for CNTF, *P = 0.007 for PACAP, independent-samples t -test), but contrast to CNTF, KO NPCs did not increase GFAP immunoreactivity even after PACAP treatment compared to WT NPCs ( ## P = 0.00004, independent-samples t -test). Scale bar = 200 μm.
    Pre Adenylated, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Tandem MS of molecular ions from cell supernatants and authentic compounds. (A) adenosine monophosphate (AMP), (B) glutathione (GSH), (C) spermine, and (D) nicotinamide adenine dinucleotide (NAD + ).

    Journal:

    Article Title: UPLC-ESI-TOFMS-Based Metabolomics and Gene Expression Dynamics Inspector Self-Organizing Metabolomic Maps as Tools for Understanding the Cellular Response to Ionizing Radiation

    doi: 10.1021/ac701807v

    Figure Lengend Snippet: Tandem MS of molecular ions from cell supernatants and authentic compounds. (A) adenosine monophosphate (AMP), (B) glutathione (GSH), (C) spermine, and (D) nicotinamide adenine dinucleotide (NAD + ).

    Article Snippet: Adenosine monophosphate, debrisoquine hemisulfate, glutathione (reduced), nicotinamide, 5-oxoproline, proline, calcium phosphorylcholine chloride, guanosine 5′-monophosphate, uridine 5′-monophosphate, β -nicotinamide adenine dinucleotide, and spermine were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Mass Spectrometry

    A water soluble fraction of Smilax china L. leaf ethanol extract (wsSCLE) stimulates protein kinase A (PKA) signaling. (A) The expression of adenylate cyclase (AC) and PKA by wsSCLE treatment. Mature 3T3-L1 adipocytes were incubated with wsSCLE (0.1 and 0.25 mg/mL) for 24 h. The β-adrenergic receptors were induced (by Iso, an agonist) and inhibited (by Pro, an antagonist). (B) The induction of the phosphorylation (p) of PKA substrates (PKAs) by wsSCLE treatment. H89 treatment (10 µM) decreased wsSCLE-induced pPKAs. Data are expressed as mean ± SD of triplicate experiments, ## P

    Journal: Nutrition Research and Practice

    Article Title: Antiobesity effects of the water-soluble fraction of the ethanol extract of Smilax china L. leaf in 3T3-L1 adipocytes

    doi: 10.4162/nrp.2015.9.6.606

    Figure Lengend Snippet: A water soluble fraction of Smilax china L. leaf ethanol extract (wsSCLE) stimulates protein kinase A (PKA) signaling. (A) The expression of adenylate cyclase (AC) and PKA by wsSCLE treatment. Mature 3T3-L1 adipocytes were incubated with wsSCLE (0.1 and 0.25 mg/mL) for 24 h. The β-adrenergic receptors were induced (by Iso, an agonist) and inhibited (by Pro, an antagonist). (B) The induction of the phosphorylation (p) of PKA substrates (PKAs) by wsSCLE treatment. H89 treatment (10 µM) decreased wsSCLE-induced pPKAs. Data are expressed as mean ± SD of triplicate experiments, ## P

    Article Snippet: The membrane was placed in blocking buffer consisting of tris-buffered saline (TBS) supplemented with 0.1% tween 20 and 5% skim milk and was incubated for 2 h. The membrane was subsequently incubated in buffer containing primary antibody for 2 h. The antibodies, and dilutions used, were as follows: adenylate cyclase (1000:1) (Abcam, Cambridge, UK); PKA (2500:1); p-PKA (2000:1); p-PKA substrate (2000:1); hormone-sensitive lipase (HSL; 3000:1); GAPDH (2000:1; Santa Cruz Biotechnology, Santa Cruz, CA, USA); p-HSL (Ser563; 1000:1);and p-HSL (Ser660; 1000:1; Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Expressing, Incubation

    Conditional loss of Cilia in the adult hippocampus and cortex. ( A ) Immunofluorescence for the neuronal cilia marker adenylate cyclase III (red) in brain sections from the following regions of the brain: Ob, Ctx, and regions of the hippocampus the pyramidal layers CA1, CA3, and granule layer of the dentate gyrus (DG). Ift88 flox/flox control samples (top row) are compared to Ift88 Δ/Δ ; Emx1-cre (bottom row). Hoechst nuclear stain is in blue. Scale bar is 50 µm. ( B ) Graph indicating the amount of cilia counted in several regions of the brain in control ( Ift88 flox/flox , N = 3) and mutants ( Ift88 Δ/Δ N = 4). Statistically significant differences are indicated: Student's t-test, * p

    Journal: PLoS ONE

    Article Title: Hippocampal and Cortical Primary Cilia Are Required for Aversive Memory in Mice

    doi: 10.1371/journal.pone.0106576

    Figure Lengend Snippet: Conditional loss of Cilia in the adult hippocampus and cortex. ( A ) Immunofluorescence for the neuronal cilia marker adenylate cyclase III (red) in brain sections from the following regions of the brain: Ob, Ctx, and regions of the hippocampus the pyramidal layers CA1, CA3, and granule layer of the dentate gyrus (DG). Ift88 flox/flox control samples (top row) are compared to Ift88 Δ/Δ ; Emx1-cre (bottom row). Hoechst nuclear stain is in blue. Scale bar is 50 µm. ( B ) Graph indicating the amount of cilia counted in several regions of the brain in control ( Ift88 flox/flox , N = 3) and mutants ( Ift88 Δ/Δ N = 4). Statistically significant differences are indicated: Student's t-test, * p

    Article Snippet: The following primary antibodies and dilutions were used: anti-actin (A2066; Sigma-Aldrich) 1∶1,000, anti-Adenylate cyclase III (sc-588; Santa Cruz Biotechnology, Santa Cruz, CA, USA) 1∶500, and anti-Ift88 rabbit polyclonal 1∶500 (Yoder Laboratory ).

    Techniques: Immunofluorescence, Marker, Staining

    A schematic representation of the sample preparation workflow. The processes of the TruSeq™ RNA Sample Preparation v2 low sample (LS) protocol (Illumina) performed manually and adopted for automated use with the SX-8G IP-Star® Compact System are illustrated. The automated protocol minimizes the hands-on time required for the error-prone manual steps of RNA-seq library synthesis, adenylation, and adaptor ligation including all related clean up steps and allows experimental multitasking for the researcher in task.

    Journal: BMC Research Notes

    Article Title: An automated method for efficient, accurate and reproducible construction of RNA-seq libraries

    doi: 10.1186/s13104-015-1089-9

    Figure Lengend Snippet: A schematic representation of the sample preparation workflow. The processes of the TruSeq™ RNA Sample Preparation v2 low sample (LS) protocol (Illumina) performed manually and adopted for automated use with the SX-8G IP-Star® Compact System are illustrated. The automated protocol minimizes the hands-on time required for the error-prone manual steps of RNA-seq library synthesis, adenylation, and adaptor ligation including all related clean up steps and allows experimental multitasking for the researcher in task.

    Article Snippet: Use of the SX-8G IP-Star® Compact System significantly reduced the hands-on time for RNA-seq library synthesis, adenylation, and adaptor ligation providing with high quality RNA-seq libraries tailored for Illumina high-throughput next-generation sequencing.

    Techniques: Sample Prep, RNA Sequencing Assay, Ligation

    RPS23RG family proteins interact with adenylate cyclases, activate PKA and reduce GSK-3 activity, Aβ level and tau phosphorylation. ( A ) RPS23RG family proteins interact with adenylate cyclase 8 (AC8). N2a cells were transfected with RPS23RG1, RPS23RG2 or RPS23RG2CΔ37, all of which are Myc-tagged, together with AC8. Co-immunoprecipitation assays in these cells showed that an adenylate cyclase (AC) antibody pulled down RPS23RG1, RPS23RG2 and RPS23RG2CΔ37 as the Myc antibody 9E10 did (upper panel), and that the Myc antibody pulled down AC as the AC antibody did (lower panel). However, mouse IgG (mIgG) and rabbit IgG (rIgG) did not pull down RPS23RG family proteins or AC. *Non-specific bands. Note that rIgG pulled down a non-specific band that is localized slightly higher than the AC band. ( B ) RPS23RG family proteins modulate the activities of PKA and GSK-3, APP processing/Aβ level and tau phosphorylation. Overexpression of RPS23RG1, RPS23RG2 and RPS23RG2CΔ37 in human HeLa cells stably expressing human APP Swedish mutation increased the levels of phosphorylated CREB indicative of upregulated PKA activity, phosphorylated GSK-3α/β indicative of reduced GSK-3 activity, sAPPα and APP CTFs, and decreased Aβ level and tau phosphorylation. p: phosphorylated forms. In some experiments, transfected cells were subjected to biotinylation and affinity precipitation to detect cell surface levels of transfected proteins. In some experiments, protein levels were quantified by densitometry and normalized to those of controls for comparison (set as one arbitrary unit). Error bars indicate SEM. # P

    Journal: Human Molecular Genetics

    Article Title: The Rps23rg gene family originated through retroposition of the ribosomal protein s23 mRNA and encodes proteins that decrease Alzheimer's ?-amyloid level and tau phosphorylation

    doi: 10.1093/hmg/ddq302

    Figure Lengend Snippet: RPS23RG family proteins interact with adenylate cyclases, activate PKA and reduce GSK-3 activity, Aβ level and tau phosphorylation. ( A ) RPS23RG family proteins interact with adenylate cyclase 8 (AC8). N2a cells were transfected with RPS23RG1, RPS23RG2 or RPS23RG2CΔ37, all of which are Myc-tagged, together with AC8. Co-immunoprecipitation assays in these cells showed that an adenylate cyclase (AC) antibody pulled down RPS23RG1, RPS23RG2 and RPS23RG2CΔ37 as the Myc antibody 9E10 did (upper panel), and that the Myc antibody pulled down AC as the AC antibody did (lower panel). However, mouse IgG (mIgG) and rabbit IgG (rIgG) did not pull down RPS23RG family proteins or AC. *Non-specific bands. Note that rIgG pulled down a non-specific band that is localized slightly higher than the AC band. ( B ) RPS23RG family proteins modulate the activities of PKA and GSK-3, APP processing/Aβ level and tau phosphorylation. Overexpression of RPS23RG1, RPS23RG2 and RPS23RG2CΔ37 in human HeLa cells stably expressing human APP Swedish mutation increased the levels of phosphorylated CREB indicative of upregulated PKA activity, phosphorylated GSK-3α/β indicative of reduced GSK-3 activity, sAPPα and APP CTFs, and decreased Aβ level and tau phosphorylation. p: phosphorylated forms. In some experiments, transfected cells were subjected to biotinylation and affinity precipitation to detect cell surface levels of transfected proteins. In some experiments, protein levels were quantified by densitometry and normalized to those of controls for comparison (set as one arbitrary unit). Error bars indicate SEM. # P

    Article Snippet: Lysates were immunoprecipitated using mouse IgG, rabbit IgG and antibodies against Myc or adenylate cyclases (Santa Cruz Biotechnology) and Trueblot™ IP beads (eBioscience, San Diego, CA, USA), followed by immunoblot with the Myc antibody.

    Techniques: Activity Assay, Transfection, Immunoprecipitation, Over Expression, Stable Transfection, Expressing, Mutagenesis, Affinity Precipitation

    DIDS abolishes pathology-induced membrane degradation. Stress-induced vital dye uptake and adenylate kinase release are prevented by DIDS treatment. ( A ) Summary of the effect of DIDS on stress-induced propidium iodide (PI) uptake. ( B C ) Dose-response relationship of IS- (B) or STS-mediated (C) PI uptake vs. [DIDS]. ( D ) Confocal fluorescence images of PI fluorescence (red) from neurons treated as indicated. Nuclei were stained with DAPI (blue) for co-localization analysis. Arrows indicate representative neurons that have taken up PI. ( E ) Summary of the effect of DIDS on stress-induced trypan blue (TB) uptake. ( F ) Summary of the effect of DIDS on stress-induced adenylate kinase (AK) release. ( G ) Summary of plasma membrane widths measured from TEM analysis. Data are mean ±SEM from 3-10 separate 24-hr experiments. Asterisks (*) indicate significant difference from normoxic controls; black bars indicate significance between connected treatments ( p

    Journal: PLoS ONE

    Article Title: DIDS Prevents Ischemic Membrane Degradation in Cultured Hippocampal Neurons by Inhibiting Matrix Metalloproteinase Release

    doi: 10.1371/journal.pone.0043995

    Figure Lengend Snippet: DIDS abolishes pathology-induced membrane degradation. Stress-induced vital dye uptake and adenylate kinase release are prevented by DIDS treatment. ( A ) Summary of the effect of DIDS on stress-induced propidium iodide (PI) uptake. ( B C ) Dose-response relationship of IS- (B) or STS-mediated (C) PI uptake vs. [DIDS]. ( D ) Confocal fluorescence images of PI fluorescence (red) from neurons treated as indicated. Nuclei were stained with DAPI (blue) for co-localization analysis. Arrows indicate representative neurons that have taken up PI. ( E ) Summary of the effect of DIDS on stress-induced trypan blue (TB) uptake. ( F ) Summary of the effect of DIDS on stress-induced adenylate kinase (AK) release. ( G ) Summary of plasma membrane widths measured from TEM analysis. Data are mean ±SEM from 3-10 separate 24-hr experiments. Asterisks (*) indicate significant difference from normoxic controls; black bars indicate significance between connected treatments ( p

    Article Snippet: Adenylate kinase membrane viability assay Assessment of the leakage of bulky adenylate kinase (AK) through plasma membranes was measured using a 96-well Toxilight microplate kit according to the manufacturer's instructions (Lonza).

    Techniques: Fluorescence, Staining, Transmission Electron Microscopy

    Adenylate kinase activities of MRSA cultures treated with different concentrations of azalomycin F 5a . MRSA ATCC 33592 cultures, respectively, treated with 1.0, 2.0, 4.0, and 8.0 μ g/mL of azalomycin F 5a , were incubated at 37°C for 6 h, and their luminescence ( n = 3) was recorded using a SpectraMax M5 microplate reader. ∗ indicates a significant difference between blank control and azalomycin F 5a group with specific concentration ( P ≤ 0.05).

    Journal: BioMed Research International

    Article Title: Mechanism of Azalomycin F5a against Methicillin-Resistant Staphylococcus aureus

    doi: 10.1155/2018/6942452

    Figure Lengend Snippet: Adenylate kinase activities of MRSA cultures treated with different concentrations of azalomycin F 5a . MRSA ATCC 33592 cultures, respectively, treated with 1.0, 2.0, 4.0, and 8.0 μ g/mL of azalomycin F 5a , were incubated at 37°C for 6 h, and their luminescence ( n = 3) was recorded using a SpectraMax M5 microplate reader. ∗ indicates a significant difference between blank control and azalomycin F 5a group with specific concentration ( P ≤ 0.05).

    Article Snippet: Finally, 100 μ L of adenylate kinase reagent (Lonza Rockland, Inc., USA) was added to each well according to the instruction of ToxiLight® BioAssay Kit and incubated 30 min at room temperature in darkness.

    Techniques: Incubation, Concentration Assay

    Epac2 is involved in PACAP-induced astrocytogenesis. (A) GFAP immunolabeling of neural precursor cells (NPCs) at day 0 (DAT0). NPCs from neither wild-type (WT) nor Epac2-knockout (KO) mice expressed GFAP at DAT0. (B) WT NPCs remarkably differentiated into astrocytes when cultured in bFGF-free media for 4 days (at DAT4), but KO NPCs showed much lower GFAP immunoreactivity than WT NPCs. (C) CNTF (50 ng/ml) induced differentiation of NPCs of both genotypes into astrocytes. (D) WT NPCs incubated in PACAP (100 nM)-treated media for 4 days expressed GFAP, but KO NPCs did not display astrocytic differentiation. (E) Ratio of GFAP-immunopositive cell number to total NPCs number. KO NPCs did not show GFAP-immunopositive astrocytes by DAT4 in bFGF-free media ( # P = 0.0001, independent-samples t -test, trial number = 8). Treatment with either CNTF or PACAP increased differentiation of WT NPCs into astrocytes compared to WT NPCs without treatment at DAT4 (*P = 0.003 for CNTF, *P = 0.002 for PACAP, independent-samples t -test, trial number in each experiment = 8), but KO NPCs did not induce astrocytic differentiation after PACAP treatment contrast to CNTF treatment ( ## P = 0.0002, independent-samples t -test, trial number = 8). (F) GFAP intensity of immunopositive cells. KO NPCs showed much lower intensity in GFAP immunoreactivity compared to WT NPCs at DAT4 in bFGF-free media ( # P = 0.0066, independent-samples t -test). Treatment with either CNTF or PACAP increased GFAP intensity in WT NPCs compared to WT NPCs without treatment at DAT4 (*P = 0.024 for CNTF, *P = 0.007 for PACAP, independent-samples t -test), but contrast to CNTF, KO NPCs did not increase GFAP immunoreactivity even after PACAP treatment compared to WT NPCs ( ## P = 0.00004, independent-samples t -test). Scale bar = 200 μm.

    Journal: BMB Reports

    Article Title: Epac2 contributes to PACAP-induced astrocytic differentiation through calcium ion influx in neural precursor cells

    doi: 10.5483/BMBRep.2016.49.2.202

    Figure Lengend Snippet: Epac2 is involved in PACAP-induced astrocytogenesis. (A) GFAP immunolabeling of neural precursor cells (NPCs) at day 0 (DAT0). NPCs from neither wild-type (WT) nor Epac2-knockout (KO) mice expressed GFAP at DAT0. (B) WT NPCs remarkably differentiated into astrocytes when cultured in bFGF-free media for 4 days (at DAT4), but KO NPCs showed much lower GFAP immunoreactivity than WT NPCs. (C) CNTF (50 ng/ml) induced differentiation of NPCs of both genotypes into astrocytes. (D) WT NPCs incubated in PACAP (100 nM)-treated media for 4 days expressed GFAP, but KO NPCs did not display astrocytic differentiation. (E) Ratio of GFAP-immunopositive cell number to total NPCs number. KO NPCs did not show GFAP-immunopositive astrocytes by DAT4 in bFGF-free media ( # P = 0.0001, independent-samples t -test, trial number = 8). Treatment with either CNTF or PACAP increased differentiation of WT NPCs into astrocytes compared to WT NPCs without treatment at DAT4 (*P = 0.003 for CNTF, *P = 0.002 for PACAP, independent-samples t -test, trial number in each experiment = 8), but KO NPCs did not induce astrocytic differentiation after PACAP treatment contrast to CNTF treatment ( ## P = 0.0002, independent-samples t -test, trial number = 8). (F) GFAP intensity of immunopositive cells. KO NPCs showed much lower intensity in GFAP immunoreactivity compared to WT NPCs at DAT4 in bFGF-free media ( # P = 0.0066, independent-samples t -test). Treatment with either CNTF or PACAP increased GFAP intensity in WT NPCs compared to WT NPCs without treatment at DAT4 (*P = 0.024 for CNTF, *P = 0.007 for PACAP, independent-samples t -test), but contrast to CNTF, KO NPCs did not increase GFAP immunoreactivity even after PACAP treatment compared to WT NPCs ( ## P = 0.00004, independent-samples t -test). Scale bar = 200 μm.

    Article Snippet: For astrocyte differentiation, cells were cultured in DMEM with N2 or calcium-free DMEM (Gibco, Thermo Fisher, Waltham, MA) with N2, which was then replaced with bFGF-free DMEM with N2 and a differentiation factor, such as 100 nM PACAP (A1439, Sigma- Aldrich, St Louis, MO) or 50 ng/ml CNTF (1203158, PeproTech) for 4 days.

    Techniques: Immunolabeling, Knock-Out, Mouse Assay, Cell Culture, Incubation