Journal: PLoS ONE

Article Title: Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva

doi: 10.1371/journal.pone.0192398

Figure Lengend Snippet: Devices used for RT-LAMP assay to detect ZIKV.

Article Snippet: Additional purified genomic RNA from four different ZIKV strains (Asian lineage) were obtained through BEI Resources, (Manassas, VA): PRVABC59, Puerto Rican strain, NR-50244 (2.0 x 10 7 RNA copies/ml); PLCal_ZV, Thailand strain, NR-50242 (5.1 x 10 7 RNA copies/ml); FLR, Florida strain, NR-5024 (4.4 x 10 7 RNA copies/ml) and R103451, Honduras strain, NR-50358 (5.4 x 10 7 RNA copies/ml).

Techniques: RT Lamp Assay, Fluorescence, Purification

Journal: PLoS ONE

Article Title: Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva

doi: 10.1371/journal.pone.0192398

Figure Lengend Snippet: (A) Representative RT-LAMP amplification curves that target the ZIKV capsid gene. Ten-fold serial dilutions of purified genomic RNA starting at 10 ng/ul. LAMP assay performed on the Genie III device. (B) Graph of the amplification threshold Tp as a function of ZIKV viral particle concentration. Tp: time to positivity.

Article Snippet: Additional purified genomic RNA from four different ZIKV strains (Asian lineage) were obtained through BEI Resources, (Manassas, VA): PRVABC59, Puerto Rican strain, NR-50244 (2.0 x 10 7 RNA copies/ml); PLCal_ZV, Thailand strain, NR-50242 (5.1 x 10 7 RNA copies/ml); FLR, Florida strain, NR-5024 (4.4 x 10 7 RNA copies/ml) and R103451, Honduras strain, NR-50358 (5.4 x 10 7 RNA copies/ml).

Techniques: Amplification, Purification, Lamp Assay, Concentration Assay

Journal: PLoS ONE

Article Title: Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva

doi: 10.1371/journal.pone.0192398

Figure Lengend Snippet: Limit of detection of RT-LAMP assay for ZIKV RNA from Asian-lineages ZIKV strains.

Article Snippet: Additional purified genomic RNA from four different ZIKV strains (Asian lineage) were obtained through BEI Resources, (Manassas, VA): PRVABC59, Puerto Rican strain, NR-50244 (2.0 x 10 7 RNA copies/ml); PLCal_ZV, Thailand strain, NR-50242 (5.1 x 10 7 RNA copies/ml); FLR, Florida strain, NR-5024 (4.4 x 10 7 RNA copies/ml) and R103451, Honduras strain, NR-50358 (5.4 x 10 7 RNA copies/ml).

Techniques: RT Lamp Assay

Journal: PLoS ONE

Article Title: Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva

doi: 10.1371/journal.pone.0192398

Figure Lengend Snippet: Each spot on the array represents duplicate immobilized Probe 4 ranging from a concentration of 20 μM to 0.039 μM by two-fold dilutions as described in . A total of 3 replicates of saliva spiked with 6 ZIKV RNA copies per reaction were evaluated along with negative controls (1XPBS as a template).

Article Snippet: Additional purified genomic RNA from four different ZIKV strains (Asian lineage) were obtained through BEI Resources, (Manassas, VA): PRVABC59, Puerto Rican strain, NR-50244 (2.0 x 10 7 RNA copies/ml); PLCal_ZV, Thailand strain, NR-50242 (5.1 x 10 7 RNA copies/ml); FLR, Florida strain, NR-5024 (4.4 x 10 7 RNA copies/ml) and R103451, Honduras strain, NR-50358 (5.4 x 10 7 RNA copies/ml).

Techniques: Concentration Assay