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  • 99
    Millipore adc growth supplement
    Adc Growth Supplement, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adc growth supplement/product/Millipore
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    adc growth supplement - by Bioz Stars, 2020-04
    99/100 stars
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    91
    Thermo Fisher gadl1 rs17026688 genotyping
    Gadl1 Rs17026688 Genotyping, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 2 article reviews
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    gadl1 rs17026688 genotyping - by Bioz Stars, 2020-04
    91/100 stars
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    87
    Thermo Fisher gadl1 antibody
    A cartoon evolutionary diagram showing the appearance of GADs, CSADC, ADC, and <t>GADL1</t> during species evolution. The assignment of proteins in different classes was based on the overall sequence similarity with typical human GAD1 (GAD67), GAD2 (GAD65),
    Gadl1 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 11 article reviews
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    91
    Thermo Fisher remel adc enrichment
    A cartoon evolutionary diagram showing the appearance of GADs, CSADC, ADC, and <t>GADL1</t> during species evolution. The assignment of proteins in different classes was based on the overall sequence similarity with typical human GAD1 (GAD67), GAD2 (GAD65),
    Remel Adc Enrichment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/remel adc enrichment/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
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    remel adc enrichment - by Bioz Stars, 2020-04
    91/100 stars
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    86
    Difco 7h10 adc
    A cartoon evolutionary diagram showing the appearance of GADs, CSADC, ADC, and <t>GADL1</t> during species evolution. The assignment of proteins in different classes was based on the overall sequence similarity with typical human GAD1 (GAD67), GAD2 (GAD65),
    7h10 Adc, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 adc/product/Difco
    Average 86 stars, based on 12 article reviews
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    7h10 adc - by Bioz Stars, 2020-04
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    92
    MiddleBrook Pharmaceuticals 7h9 adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    7h9 Adc, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 13 article reviews
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    7h9 adc - by Bioz Stars, 2020-04
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    92
    MiddleBrook Pharmaceuticals adc middlebrook
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Adc Middlebrook, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 7 article reviews
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    adc middlebrook - by Bioz Stars, 2020-04
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    93
    Difco adc supplement
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Adc Supplement, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adc supplement/product/Difco
    Average 93 stars, based on 58 article reviews
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    adc supplement - by Bioz Stars, 2020-04
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    85
    Beckman Coulter expo32 adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Expo32 Adc, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 3 article reviews
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    expo32 adc - by Bioz Stars, 2020-04
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    94
    Difco middlebrook adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Middlebrook Adc, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 19 article reviews
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    middlebrook adc - by Bioz Stars, 2020-04
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    93
    Becton Dickinson middlebrook adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Middlebrook Adc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 15 article reviews
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    middlebrook adc - by Bioz Stars, 2020-04
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    93
    Thermo Fisher middlebrook adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Middlebrook Adc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook adc/product/Thermo Fisher
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    middlebrook adc - by Bioz Stars, 2020-04
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    93
    Texas Instruments ads1291 adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Ads1291 Adc, supplied by Texas Instruments, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ads1291 adc/product/Texas Instruments
    Average 93 stars, based on 1 article reviews
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    ads1291 adc - by Bioz Stars, 2020-04
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    80
    Pointe Scientific alazartech adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Alazartech Adc, supplied by Pointe Scientific, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 1 article reviews
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    alazartech adc - by Bioz Stars, 2020-04
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    90
    Texas Instruments msp430f149 adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Msp430f149 Adc, supplied by Texas Instruments, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 2 article reviews
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    msp430f149 adc - by Bioz Stars, 2020-04
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    91
    Becton Dickinson adc supplement
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Adc Supplement, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 23 article reviews
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    adc supplement - by Bioz Stars, 2020-04
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    86
    Thermo Fisher adc supplement
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Adc Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    adc supplement - by Bioz Stars, 2020-04
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    94
    Ambrx αher2 adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    αher2 Adc, supplied by Ambrx, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 10 article reviews
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    αher2 adc - by Bioz Stars, 2020-04
    94/100 stars
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    80
    Becton Dickinson adc additive
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Adc Additive, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adc additive/product/Becton Dickinson
    Average 80 stars, based on 1 article reviews
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    adc additive - by Bioz Stars, 2020-04
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    84
    Avantor middlebrook adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Middlebrook Adc, supplied by Avantor, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 84 stars, based on 2 article reviews
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    middlebrook adc - by Bioz Stars, 2020-04
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    85
    Texas Instruments msps adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Msps Adc, supplied by Texas Instruments, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    msps adc - by Bioz Stars, 2020-04
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    92
    Difco catalase adc
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Catalase Adc, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/catalase adc/product/Difco
    Average 92 stars, based on 8 article reviews
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    catalase adc - by Bioz Stars, 2020-04
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    91
    Molecular Devices LLC dac adc interface
    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified <t>7H9/ADC</t> [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.
    Dac Adc Interface, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dac adc interface/product/Molecular Devices LLC
    Average 91 stars, based on 16 article reviews
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    dac adc interface - by Bioz Stars, 2020-04
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    94
    Becton Dickinson adc
    Intracellular survival of mutants compared to WT in human monocytes. Human blood monocytes (1.0x10 6 ) from healthy volunteers were infected (MOI 10) with mutants and WT. Intracellular bacteria were quantified after 4 hour of infection, and after 1, 2, 4 days. The monocytes were lysed in 1 ml of sterile water and 100 μl of 1:500 dilution in sterile water of sample were plated on <t>Middlebrook</t> agar plates supplemented with <t>ADC</t> for CFU counting. A : WT and mutant MAV_4334; B : WT and mutant MAV_5106; C : WT and mutant MAV_1778; D : WT and mutant MAV_3128. Statistical analysis was done using a two tailed, paired Student’s t test. When compared to wild-type a P
    Adc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 220 article reviews
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    adc - by Bioz Stars, 2020-04
    94/100 stars
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    adc  (Difco)
    93
    Difco adc
    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid <t>7H9</t> medium supplemented with 0.05% Tween 80, <t>ADC</t> supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).
    Adc, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adc/product/Difco
    Average 93 stars, based on 151 article reviews
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    adc - by Bioz Stars, 2020-04
    93/100 stars
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    92
    Genentech adc
    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid <t>7H9</t> medium supplemented with 0.05% Tween 80, <t>ADC</t> supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).
    Adc, supplied by Genentech, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 20 article reviews
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    adc - by Bioz Stars, 2020-04
    92/100 stars
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    adc  (Genovis)
    93
    Genovis adc
    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid <t>7H9</t> medium supplemented with 0.05% Tween 80, <t>ADC</t> supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).
    Adc, supplied by Genovis, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adc/product/Genovis
    Average 93 stars, based on 6 article reviews
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    adc - by Bioz Stars, 2020-04
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    92
    HiMedia Laboratories adc
    M. smegmatis expressing PPE2 with intact NLS inhibited NO production and survived significantly longer inside macrophages. About 0.5 million peritoneal macrophages from C57BL/6 mice ( A and C ) or RAW 264.7 macrophages ( B and D ) were infected with either M. smegmatis harbouring pVV16 vector alone (Msmeg-pVV16), or pVV16-PPE2 (Msmeg-PPE2) or pVV16-ΔNLS-PPE2 (Msmeg-ΔNLS-PPE2) at 1:10 multiplicities of infection (MOI) and supernatants were removed for quantification of nitrite accumulation at 24 and 48 hours ( A and B ). The infected cells were lysed at 4, 24 and 48 hour post-infection. The cell lysates were plated on <t>7H10</t> plates supplemented with 10% <t>ADC</t> and 0.05% of Tween 80 and incubated for 4 days for counting the number of colony forming units (CFUs) ( C and D ). Data were expressed as mean ± SD of 3 independent experiments.
    Adc, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 10 article reviews
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    adc - by Bioz Stars, 2020-04
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    92
    Merck & Co adc
    M. smegmatis expressing PPE2 with intact NLS inhibited NO production and survived significantly longer inside macrophages. About 0.5 million peritoneal macrophages from C57BL/6 mice ( A and C ) or RAW 264.7 macrophages ( B and D ) were infected with either M. smegmatis harbouring pVV16 vector alone (Msmeg-pVV16), or pVV16-PPE2 (Msmeg-PPE2) or pVV16-ΔNLS-PPE2 (Msmeg-ΔNLS-PPE2) at 1:10 multiplicities of infection (MOI) and supernatants were removed for quantification of nitrite accumulation at 24 and 48 hours ( A and B ). The infected cells were lysed at 4, 24 and 48 hour post-infection. The cell lysates were plated on <t>7H10</t> plates supplemented with 10% <t>ADC</t> and 0.05% of Tween 80 and incubated for 4 days for counting the number of colony forming units (CFUs) ( C and D ). Data were expressed as mean ± SD of 3 independent experiments.
    Adc, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    adc - by Bioz Stars, 2020-04
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    93
    Millipore adc
    The mRNA expression of <t>KP-OVA-52</t> in cancer cell lines. Cancer cell lines were treated for 5 days with or without 1 μ M <t>ADC.</t> RT-PCR amplifications were performed with primers specific to the KP-OVA-52 gene. GAPDH was used as a loading control for RT-PCR. The ten cell lines used were nine KP-OVA-52 non-expressing cell lines (SNU-C1, SK-LC-5, SK-LC-14, NCI-H189, NCI-H82, SNU-C2A, NCI-H23, MCF-7 and NCI-H146) and expressing cell line (SK-OV-3).
    Adc, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 60 article reviews
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    adc - by Bioz Stars, 2020-04
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    Image Search Results


    A cartoon evolutionary diagram showing the appearance of GADs, CSADC, ADC, and GADL1 during species evolution. The assignment of proteins in different classes was based on the overall sequence similarity with typical human GAD1 (GAD67), GAD2 (GAD65),

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Glutamate Decarboxylase-like Protein 1 (GADL1) in Taurine Biosynthesis *

    doi: 10.1074/jbc.M112.393728

    Figure Lengend Snippet: A cartoon evolutionary diagram showing the appearance of GADs, CSADC, ADC, and GADL1 during species evolution. The assignment of proteins in different classes was based on the overall sequence similarity with typical human GAD1 (GAD67), GAD2 (GAD65),

    Article Snippet: The membrane was blocked with 5% nonfat dried milk in TBST buffer (20 m m Tris-HCl, 500 m m NaCl, and 0.05% Tween 20) and incubated with GADL1 antibody (Thermo Scientific) at 1:100 dilution in TBST with 5% BSA overnight at 4 °C.

    Techniques: Sequencing

    RT-PCR analysis of GADL1 in mice and cattle tissues. The tissue distribution of GADL1 mRNA was studied in mice ( A ) and cattle ( B ). Ribosomal 18 S RNA was used as an internal standard for both species.

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Glutamate Decarboxylase-like Protein 1 (GADL1) in Taurine Biosynthesis *

    doi: 10.1074/jbc.M112.393728

    Figure Lengend Snippet: RT-PCR analysis of GADL1 in mice and cattle tissues. The tissue distribution of GADL1 mRNA was studied in mice ( A ) and cattle ( B ). Ribosomal 18 S RNA was used as an internal standard for both species.

    Article Snippet: The membrane was blocked with 5% nonfat dried milk in TBST buffer (20 m m Tris-HCl, 500 m m NaCl, and 0.05% Tween 20) and incubated with GADL1 antibody (Thermo Scientific) at 1:100 dilution in TBST with 5% BSA overnight at 4 °C.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Mouse Assay

    Western blot analysis of GADL1 in tissues and cell culture. A , the presence of GADL1 in mouse kidneys and muscles. Noticed that a lower band was detected in kidneys; this band corresponded to CSADC. B , the presence of GADL1 in mouse C2C12 myoblasts and

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Glutamate Decarboxylase-like Protein 1 (GADL1) in Taurine Biosynthesis *

    doi: 10.1074/jbc.M112.393728

    Figure Lengend Snippet: Western blot analysis of GADL1 in tissues and cell culture. A , the presence of GADL1 in mouse kidneys and muscles. Noticed that a lower band was detected in kidneys; this band corresponded to CSADC. B , the presence of GADL1 in mouse C2C12 myoblasts and

    Article Snippet: The membrane was blocked with 5% nonfat dried milk in TBST buffer (20 m m Tris-HCl, 500 m m NaCl, and 0.05% Tween 20) and incubated with GADL1 antibody (Thermo Scientific) at 1:100 dilution in TBST with 5% BSA overnight at 4 °C.

    Techniques: Western Blot, Cell Culture

    Spectral characteristics of recombinant Hu GADL1. Extensively purified Hu GADL1 was prepared in 20 m m phosphate buffer (pH 7.0), and its absorbance from 300 to 480 nm was determined using a Hitachi U-2800A spectrophotometer. The inset illustrates purified

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Glutamate Decarboxylase-like Protein 1 (GADL1) in Taurine Biosynthesis *

    doi: 10.1074/jbc.M112.393728

    Figure Lengend Snippet: Spectral characteristics of recombinant Hu GADL1. Extensively purified Hu GADL1 was prepared in 20 m m phosphate buffer (pH 7.0), and its absorbance from 300 to 480 nm was determined using a Hitachi U-2800A spectrophotometer. The inset illustrates purified

    Article Snippet: The membrane was blocked with 5% nonfat dried milk in TBST buffer (20 m m Tris-HCl, 500 m m NaCl, and 0.05% Tween 20) and incubated with GADL1 antibody (Thermo Scientific) at 1:100 dilution in TBST with 5% BSA overnight at 4 °C.

    Techniques: Recombinant, Purification, Spectrophotometry

    Substrate specificities of recombinant Hu GADL1. Chromatograms A , B , and C show the accumulation of hypotaurine in a Hu GADL1 and cysteine sulfinic acid reaction mixture, the accumulation of taurine in a Hu GADL1 and cysteic acid reaction mixture, and the

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Glutamate Decarboxylase-like Protein 1 (GADL1) in Taurine Biosynthesis *

    doi: 10.1074/jbc.M112.393728

    Figure Lengend Snippet: Substrate specificities of recombinant Hu GADL1. Chromatograms A , B , and C show the accumulation of hypotaurine in a Hu GADL1 and cysteine sulfinic acid reaction mixture, the accumulation of taurine in a Hu GADL1 and cysteic acid reaction mixture, and the

    Article Snippet: The membrane was blocked with 5% nonfat dried milk in TBST buffer (20 m m Tris-HCl, 500 m m NaCl, and 0.05% Tween 20) and incubated with GADL1 antibody (Thermo Scientific) at 1:100 dilution in TBST with 5% BSA overnight at 4 °C.

    Techniques: Recombinant

    Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified 7H9/ADC [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.

    Journal: Infection and Immunity

    Article Title: A Mutation in the 16S rRNA Decoding Region Attenuates the Virulence of Mycobacterium tuberculosis

    doi: 10.1128/IAI.00417-16

    Figure Lengend Snippet: Growth curves of NCGM2242 and Erdman in MycoBroth. The growth of the indicated strains in MycoBroth (modified 7H9/ADC [see Materials and Methods]) was monitored by measuring the OD 530 . Time ( y axis) was measured in days (d). Data are means ± standard deviations of results from three independent experiments.

    Article Snippet: The growth speeds of NCGM2242 and NCGM2242(pMV261Zeo) (mock) were identical in 7H9/ADC.

    Techniques: Modification

    Complementation by the wild-type rRNA operon. (A) Growth curves of strains Erdman, NCGM2242, NCGM2242(pMV261Zeo), and NCGM2242(pMV261Zeo-rrsWT) in 7H9/ADC medium. Data are means ± standard deviations of results from three independent experiments. (B) Expression of KsgA in Erdman, NCGM2242, NCGM2242(pMV261Zeo), and NCGM2242(pMV261Zeo-rrsWT). M. tuberculosis strains were cultured in 7H9/ADC at 37°C for 4 days with shaking. Lysates of the indicated strains were immunoblotted with anti-KsgA antibody. Asterisks indicate nonspecific bands. Arrows indicate endogenous KsgA. (C) Bacterial loads at day 94 in the spleens, livers, and lungs of BALB/c mice i nfected with strain Erdman ( n = 6), NCGM2242(pMV261Zeo-rrsWT) ( n = 6), or NCGM2242(pMV261Zeo) ( n = 5). *, P

    Journal: Infection and Immunity

    Article Title: A Mutation in the 16S rRNA Decoding Region Attenuates the Virulence of Mycobacterium tuberculosis

    doi: 10.1128/IAI.00417-16

    Figure Lengend Snippet: Complementation by the wild-type rRNA operon. (A) Growth curves of strains Erdman, NCGM2242, NCGM2242(pMV261Zeo), and NCGM2242(pMV261Zeo-rrsWT) in 7H9/ADC medium. Data are means ± standard deviations of results from three independent experiments. (B) Expression of KsgA in Erdman, NCGM2242, NCGM2242(pMV261Zeo), and NCGM2242(pMV261Zeo-rrsWT). M. tuberculosis strains were cultured in 7H9/ADC at 37°C for 4 days with shaking. Lysates of the indicated strains were immunoblotted with anti-KsgA antibody. Asterisks indicate nonspecific bands. Arrows indicate endogenous KsgA. (C) Bacterial loads at day 94 in the spleens, livers, and lungs of BALB/c mice i nfected with strain Erdman ( n = 6), NCGM2242(pMV261Zeo-rrsWT) ( n = 6), or NCGM2242(pMV261Zeo) ( n = 5). *, P

    Article Snippet: The growth speeds of NCGM2242 and NCGM2242(pMV261Zeo) (mock) were identical in 7H9/ADC.

    Techniques: Expressing, Cell Culture, Mouse Assay

    Intracellular survival of mutants compared to WT in human monocytes. Human blood monocytes (1.0x10 6 ) from healthy volunteers were infected (MOI 10) with mutants and WT. Intracellular bacteria were quantified after 4 hour of infection, and after 1, 2, 4 days. The monocytes were lysed in 1 ml of sterile water and 100 μl of 1:500 dilution in sterile water of sample were plated on Middlebrook agar plates supplemented with ADC for CFU counting. A : WT and mutant MAV_4334; B : WT and mutant MAV_5106; C : WT and mutant MAV_1778; D : WT and mutant MAV_3128. Statistical analysis was done using a two tailed, paired Student’s t test. When compared to wild-type a P

    Journal: BMC Microbiology

    Article Title: Illegitimate recombination: An efficient method for random mutagenesis in Mycobacterium avium subsp. hominissuis

    doi: 10.1186/1471-2180-12-204

    Figure Lengend Snippet: Intracellular survival of mutants compared to WT in human monocytes. Human blood monocytes (1.0x10 6 ) from healthy volunteers were infected (MOI 10) with mutants and WT. Intracellular bacteria were quantified after 4 hour of infection, and after 1, 2, 4 days. The monocytes were lysed in 1 ml of sterile water and 100 μl of 1:500 dilution in sterile water of sample were plated on Middlebrook agar plates supplemented with ADC for CFU counting. A : WT and mutant MAV_4334; B : WT and mutant MAV_5106; C : WT and mutant MAV_1778; D : WT and mutant MAV_3128. Statistical analysis was done using a two tailed, paired Student’s t test. When compared to wild-type a P

    Article Snippet: Bacterial strains, amoeba, cell lines and growth conditions Mycobacterial strains were grown in Middlebrook (MB) 7H9 broth (BD Biosciences, USA), supplemented with either 10% ADC (BD Biosciences) or 10% OADC (BD Biosciences) and 0.05% Tween 80 without shaking, and on MB 7H11 agar (BD Biosciences) at 37°C.

    Techniques: Infection, Mutagenesis, Two Tailed Test

    —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid 7H9 medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).

    Journal: Genome Biology and Evolution

    Article Title: Unexpected Genomic and Phenotypic Diversity of Mycobacterium africanum Lineage 5 Affects Drug Resistance, Protein Secretion, and Immunogenicity

    doi: 10.1093/gbe/evy145

    Figure Lengend Snippet: —Sublineage-specific growth characteristics of M. africanum sublineages. Precultures of the indicated strains were grown until midlogarithmic phase and were diluted to 0.05 OD 600 /ml in glass tubes containing 5 ml of liquid 7H9 medium supplemented with 0.05% Tween 80, ADC supplement and 0.2% pyruvate. Optical density was measured at the indicated time points (symbols). Data points are averages of three individual experiments performed in technical duplicates. Colors of line indicate the specific (sub)lineage of M. tuberculosis ( Mtb ) or M. africanum ( Maf ) on the legend (top left). Individual isolates can be identified by combination of colors and symbols used (bottom right).

    Article Snippet: Cultivation of all isolates ( , online) was performed on 7H11 solid medium with OADC supplement (Difco), or in liquid 7H9 medium supplemented with ADC (Difco), 0.05% Tween 80, and 0.2% w/v pyruvate ( ).

    Techniques:

    M. smegmatis expressing PPE2 with intact NLS inhibited NO production and survived significantly longer inside macrophages. About 0.5 million peritoneal macrophages from C57BL/6 mice ( A and C ) or RAW 264.7 macrophages ( B and D ) were infected with either M. smegmatis harbouring pVV16 vector alone (Msmeg-pVV16), or pVV16-PPE2 (Msmeg-PPE2) or pVV16-ΔNLS-PPE2 (Msmeg-ΔNLS-PPE2) at 1:10 multiplicities of infection (MOI) and supernatants were removed for quantification of nitrite accumulation at 24 and 48 hours ( A and B ). The infected cells were lysed at 4, 24 and 48 hour post-infection. The cell lysates were plated on 7H10 plates supplemented with 10% ADC and 0.05% of Tween 80 and incubated for 4 days for counting the number of colony forming units (CFUs) ( C and D ). Data were expressed as mean ± SD of 3 independent experiments.

    Journal: Scientific Reports

    Article Title: The PPE2 protein of Mycobacterium tuberculosis translocates to host nucleus and inhibits nitric oxide production

    doi: 10.1038/srep39706

    Figure Lengend Snippet: M. smegmatis expressing PPE2 with intact NLS inhibited NO production and survived significantly longer inside macrophages. About 0.5 million peritoneal macrophages from C57BL/6 mice ( A and C ) or RAW 264.7 macrophages ( B and D ) were infected with either M. smegmatis harbouring pVV16 vector alone (Msmeg-pVV16), or pVV16-PPE2 (Msmeg-PPE2) or pVV16-ΔNLS-PPE2 (Msmeg-ΔNLS-PPE2) at 1:10 multiplicities of infection (MOI) and supernatants were removed for quantification of nitrite accumulation at 24 and 48 hours ( A and B ). The infected cells were lysed at 4, 24 and 48 hour post-infection. The cell lysates were plated on 7H10 plates supplemented with 10% ADC and 0.05% of Tween 80 and incubated for 4 days for counting the number of colony forming units (CFUs) ( C and D ). Data were expressed as mean ± SD of 3 independent experiments.

    Article Snippet: Serial dilutions of cell extracts were plated on 7H10 plates supplemented with 10% ADC (HIMEDIA, India) and 25 μg/ml Kanamycin, 50 μg/ml Hygromycin.

    Techniques: Expressing, Mouse Assay, Infection, Plasmid Preparation, Incubation

    Effect of NO inhibitor, aminoguanidine (AG) and NO donor sodium nitroprusside (SNP) on survival of M. smegmatis harbouring either backbone vector (Msmeg-pVV16), or expressing PPE2 (Msmeg-PPE2) or ΔNLS-PPE2 (Msmeg-ΔNLS-PPE2). Murine peritoneal macrophages (0.5 × 10 6 ) were either cultured in medium alone or pre-treated with either AG (300 μg/ml) or SNP (100 μM) followed by infection with either Msmeg-pVV16, or Msmeg-PPE2 or Msmeg-ΔNLS-PPE2 at 1:10 multiplicities of infection (MOI). Twenty four hours after infection, supernatants were removed for nitrite determination and the cells were lysed for CFU assay. The cell lysates were plated on 7H10 plates supplemented with 10% ADC, Kanamycin (25 μg/ml), Hygromycin B (50 μg/ml) and 0.05% of Tween 80 and incubated for 4 days for counting the number of colony forming units (CFUs). Data were expressed as mean ± SD of 3 independent experiments.

    Journal: Scientific Reports

    Article Title: The PPE2 protein of Mycobacterium tuberculosis translocates to host nucleus and inhibits nitric oxide production

    doi: 10.1038/srep39706

    Figure Lengend Snippet: Effect of NO inhibitor, aminoguanidine (AG) and NO donor sodium nitroprusside (SNP) on survival of M. smegmatis harbouring either backbone vector (Msmeg-pVV16), or expressing PPE2 (Msmeg-PPE2) or ΔNLS-PPE2 (Msmeg-ΔNLS-PPE2). Murine peritoneal macrophages (0.5 × 10 6 ) were either cultured in medium alone or pre-treated with either AG (300 μg/ml) or SNP (100 μM) followed by infection with either Msmeg-pVV16, or Msmeg-PPE2 or Msmeg-ΔNLS-PPE2 at 1:10 multiplicities of infection (MOI). Twenty four hours after infection, supernatants were removed for nitrite determination and the cells were lysed for CFU assay. The cell lysates were plated on 7H10 plates supplemented with 10% ADC, Kanamycin (25 μg/ml), Hygromycin B (50 μg/ml) and 0.05% of Tween 80 and incubated for 4 days for counting the number of colony forming units (CFUs). Data were expressed as mean ± SD of 3 independent experiments.

    Article Snippet: Serial dilutions of cell extracts were plated on 7H10 plates supplemented with 10% ADC (HIMEDIA, India) and 25 μg/ml Kanamycin, 50 μg/ml Hygromycin.

    Techniques: Plasmid Preparation, Expressing, Cell Culture, Infection, Colony-forming Unit Assay, Incubation

    The mRNA expression of KP-OVA-52 in cancer cell lines. Cancer cell lines were treated for 5 days with or without 1 μ M ADC. RT-PCR amplifications were performed with primers specific to the KP-OVA-52 gene. GAPDH was used as a loading control for RT-PCR. The ten cell lines used were nine KP-OVA-52 non-expressing cell lines (SNU-C1, SK-LC-5, SK-LC-14, NCI-H189, NCI-H82, SNU-C2A, NCI-H23, MCF-7 and NCI-H146) and expressing cell line (SK-OV-3).

    Journal: International Journal of Oncology

    Article Title: A novel cancer/testis antigen KP-OVA-52 identified by SEREX in human ovarian cancer is regulated by DNA methylation

    doi: 10.3892/ijo.2012.1508

    Figure Lengend Snippet: The mRNA expression of KP-OVA-52 in cancer cell lines. Cancer cell lines were treated for 5 days with or without 1 μ M ADC. RT-PCR amplifications were performed with primers specific to the KP-OVA-52 gene. GAPDH was used as a loading control for RT-PCR. The ten cell lines used were nine KP-OVA-52 non-expressing cell lines (SNU-C1, SK-LC-5, SK-LC-14, NCI-H189, NCI-H82, SNU-C2A, NCI-H23, MCF-7 and NCI-H146) and expressing cell line (SK-OV-3).

    Article Snippet: In experiments designed to determine the effect of demethylation on the expression of KP-OVA-52 gene, actively replicating cells were treated with 1 μ M of ADC (Sigma, St. Louis, MO, USA) for 5 days.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction