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active recombinant heparanase  (R&D Systems)
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R&D Systems active recombinant heparanase
Fig. 4 HDACi upregulate <t>heparanase</t> mRNA and protein. a Analysis of HPSE expression by qRT-PCR in SS cells treated with SAHA or FK228 for the indicated times. Data are reported as relative quantification with respect to untreated cells as calibration sample. Relative mRNA values are the mean ± SE from three independent experiments. b Western blot detection of heparanase polypeptides in CME-1 cells exposed to SAHA or FK228 for the indicated times. Image on the right is from a cropped blot (dashed line) from which lanes not of interest have been removed. c N-glycosylation inhibition does not modify electrophoretic mobility of heparanase polypeptides. CME-1 cells were treated with tunicamycin (2 μg/ ml for 24 h) and processed for Western blotting. As controls, the shift of PDGFRα bands shows the glycosylation inhibition and BIP upregulation indicates endoplasmic reticulum stress. In (b) and (c) anti-actin, −vinculin and -GAPDH blots are shown as loading control
Active Recombinant Heparanase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/active recombinant heparanase/product/R&D Systems
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active recombinant heparanase - by Bioz Stars, 2026-05
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human active heparanase  (R&D Systems)
94
R&D Systems human active heparanase
MSC hpa promotes vascular regeneration in vivo. (A) : Representative Laser-Doppler images (LDI) of hind limbs before, immediately after, and 3, 7, and 14 days after femoral artery occlusion. (B) : Quantitative LDI analysis showing the right-to-left ( R/L ) ratio; n ≥ for each group, * denotes p < .05. (C) : Detection of <t>heparanase</t> protein expression using Western blot in WT MSC, MSC harboring empty vector, and heparanase over-expressed vector. (D) : Detection of heparanase by ELISA in conditioned medium of WT MSC, MSC harboring empty vector, and heparanase over-expressed vector; n = 4 for each group, * denotes p < .05. (E) : HE and immunofluorescent staining of α -SMA and CD31 in muscle tissues from each group; Bar = 100 μm for HE and Bar = 50 μm for immunofluorescent staining. (F, G) : Bar graph showed quantitative analysis of SMA and CD31 positive area density; n = 5 for each group, * denotes p < .05; ** denotes p < .01. Abbreviations: MSC, mesenchymal stem cell; PBS, phosphate buffer saline; SMA, smooth muscle actin.
Human Active Heparanase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human active heparanase/product/R&D Systems
Average 94 stars, based on 1 article reviews
human active heparanase - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
human heparanase 1 hpse activation kit by crispra  (OriGene)
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HPSE CRISPRa kit CRISPR gene activation of human heparanase
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recombinant human active heparanase hpse protein cf  (R&D Systems)
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The Recombinant Human Active Heparanase HPSE Protein from R D Systems is derived from CHO The Recombinant Human Active Heparanase HPSE Protein has been validated for the following applications Enzyme Activity
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Fig. 4 HDACi upregulate heparanase mRNA and protein. a Analysis of HPSE expression by qRT-PCR in SS cells treated with SAHA or FK228 for the indicated times. Data are reported as relative quantification with respect to untreated cells as calibration sample. Relative mRNA values are the mean ± SE from three independent experiments. b Western blot detection of heparanase polypeptides in CME-1 cells exposed to SAHA or FK228 for the indicated times. Image on the right is from a cropped blot (dashed line) from which lanes not of interest have been removed. c N-glycosylation inhibition does not modify electrophoretic mobility of heparanase polypeptides. CME-1 cells were treated with tunicamycin (2 μg/ ml for 24 h) and processed for Western blotting. As controls, the shift of PDGFRα bands shows the glycosylation inhibition and BIP upregulation indicates endoplasmic reticulum stress. In (b) and (c) anti-actin, −vinculin and -GAPDH blots are shown as loading control

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Upregulation of ERK-EGR1-heparanase axis by HDAC inhibitors provides targets for rational therapeutic intervention in synovial sarcoma.

doi: 10.1186/s13046-021-02150-y

Figure Lengend Snippet: Fig. 4 HDACi upregulate heparanase mRNA and protein. a Analysis of HPSE expression by qRT-PCR in SS cells treated with SAHA or FK228 for the indicated times. Data are reported as relative quantification with respect to untreated cells as calibration sample. Relative mRNA values are the mean ± SE from three independent experiments. b Western blot detection of heparanase polypeptides in CME-1 cells exposed to SAHA or FK228 for the indicated times. Image on the right is from a cropped blot (dashed line) from which lanes not of interest have been removed. c N-glycosylation inhibition does not modify electrophoretic mobility of heparanase polypeptides. CME-1 cells were treated with tunicamycin (2 μg/ ml for 24 h) and processed for Western blotting. As controls, the shift of PDGFRα bands shows the glycosylation inhibition and BIP upregulation indicates endoplasmic reticulum stress. In (b) and (c) anti-actin, −vinculin and -GAPDH blots are shown as loading control

Article Snippet: For treatment with human active recombinant heparanase (R&D systems, Minneapolis, MN) (Supplementary Table S1), cells plated in complete medium for 24 h were incubated in serum-free medium with or without 5 μg/ml recombinant enzyme for 24 h and 48 h.

Techniques: Expressing, Quantitative RT-PCR, Quantitative Proteomics, Western Blot, Glycoproteomics, Inhibition, Control

Fig. 9 Schematic representation of the proposed HDACi activated auto-sustaining pro-survival loop and its blockade by co-treatment with ERK pathway and heparanase inhibitors in SS cells. This figure was prepared using tools from Servier Medical Art (http://www.servier.fr/servier-medic al-art)

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Upregulation of ERK-EGR1-heparanase axis by HDAC inhibitors provides targets for rational therapeutic intervention in synovial sarcoma.

doi: 10.1186/s13046-021-02150-y

Figure Lengend Snippet: Fig. 9 Schematic representation of the proposed HDACi activated auto-sustaining pro-survival loop and its blockade by co-treatment with ERK pathway and heparanase inhibitors in SS cells. This figure was prepared using tools from Servier Medical Art (http://www.servier.fr/servier-medic al-art)

Article Snippet: For treatment with human active recombinant heparanase (R&D systems, Minneapolis, MN) (Supplementary Table S1), cells plated in complete medium for 24 h were incubated in serum-free medium with or without 5 μg/ml recombinant enzyme for 24 h and 48 h.

Techniques:

MSC hpa promotes vascular regeneration in vivo. (A) : Representative Laser-Doppler images (LDI) of hind limbs before, immediately after, and 3, 7, and 14 days after femoral artery occlusion. (B) : Quantitative LDI analysis showing the right-to-left ( R/L ) ratio; n ≥ for each group, * denotes p < .05. (C) : Detection of heparanase protein expression using Western blot in WT MSC, MSC harboring empty vector, and heparanase over-expressed vector. (D) : Detection of heparanase by ELISA in conditioned medium of WT MSC, MSC harboring empty vector, and heparanase over-expressed vector; n = 4 for each group, * denotes p < .05. (E) : HE and immunofluorescent staining of α -SMA and CD31 in muscle tissues from each group; Bar = 100 μm for HE and Bar = 50 μm for immunofluorescent staining. (F, G) : Bar graph showed quantitative analysis of SMA and CD31 positive area density; n = 5 for each group, * denotes p < .05; ** denotes p < .01. Abbreviations: MSC, mesenchymal stem cell; PBS, phosphate buffer saline; SMA, smooth muscle actin.

Journal: Stem cells (Dayton, Ohio)

Article Title: Heparanase Released From Mesenchymal Stem Cells Activates Integrin beta1/HIF-2alpha/Flk-1 Signaling and Promotes Endothelial Cell Migration and Angiogenesis

doi: 10.1002/stem.1995

Figure Lengend Snippet: MSC hpa promotes vascular regeneration in vivo. (A) : Representative Laser-Doppler images (LDI) of hind limbs before, immediately after, and 3, 7, and 14 days after femoral artery occlusion. (B) : Quantitative LDI analysis showing the right-to-left ( R/L ) ratio; n ≥ for each group, * denotes p < .05. (C) : Detection of heparanase protein expression using Western blot in WT MSC, MSC harboring empty vector, and heparanase over-expressed vector. (D) : Detection of heparanase by ELISA in conditioned medium of WT MSC, MSC harboring empty vector, and heparanase over-expressed vector; n = 4 for each group, * denotes p < .05. (E) : HE and immunofluorescent staining of α -SMA and CD31 in muscle tissues from each group; Bar = 100 μm for HE and Bar = 50 μm for immunofluorescent staining. (F, G) : Bar graph showed quantitative analysis of SMA and CD31 positive area density; n = 5 for each group, * denotes p < .05; ** denotes p < .01. Abbreviations: MSC, mesenchymal stem cell; PBS, phosphate buffer saline; SMA, smooth muscle actin.

Article Snippet: The lower chamber was filled with MSC-conditioned medium or control medium as above or with recombinant human active heparanase (100 mg/ml, R&D Systems, Minneapolis, MN, USA).

Techniques: In Vivo, Expressing, Western Blot, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Staining, Saline

Proangiogenic role of MSC hpa in vitro. (A) : Protein expression of heparanase and actin by Western blot in MSC null and MSC hpa-KD . (B) : Detection of heparanase concentration using ELISA in conditioned medium of MSC WT , MSC null , and MSC hpa-KD ; n = 4 for each group, * denotes p < .05. (C) : Representative images showing tube formation of human umbilical vein endothelial cells after cocultured with conditioned medium derived from MSC WT , MSC null , MSC hpa , MSC hpa-KD , respectively; Bar = 50 μm. (D) : Quantification of tube length in each group; n = 3 for each group, ** denotes p < .01. (E) : Aortic ring assay showing sprouting and branching in each group; Bar = 50 μm. (F, G) : Bar graph showed quantitative analysis of sprout length and outgrowth area, respectively; n = 3 for each group, * denotes p < .05, ** denotes p < .01. Abbreviation: MSC, mesenchymal stem cell.

Journal: Stem cells (Dayton, Ohio)

Article Title: Heparanase Released From Mesenchymal Stem Cells Activates Integrin beta1/HIF-2alpha/Flk-1 Signaling and Promotes Endothelial Cell Migration and Angiogenesis

doi: 10.1002/stem.1995

Figure Lengend Snippet: Proangiogenic role of MSC hpa in vitro. (A) : Protein expression of heparanase and actin by Western blot in MSC null and MSC hpa-KD . (B) : Detection of heparanase concentration using ELISA in conditioned medium of MSC WT , MSC null , and MSC hpa-KD ; n = 4 for each group, * denotes p < .05. (C) : Representative images showing tube formation of human umbilical vein endothelial cells after cocultured with conditioned medium derived from MSC WT , MSC null , MSC hpa , MSC hpa-KD , respectively; Bar = 50 μm. (D) : Quantification of tube length in each group; n = 3 for each group, ** denotes p < .01. (E) : Aortic ring assay showing sprouting and branching in each group; Bar = 50 μm. (F, G) : Bar graph showed quantitative analysis of sprout length and outgrowth area, respectively; n = 3 for each group, * denotes p < .05, ** denotes p < .01. Abbreviation: MSC, mesenchymal stem cell.

Article Snippet: The lower chamber was filled with MSC-conditioned medium or control medium as above or with recombinant human active heparanase (100 mg/ml, R&D Systems, Minneapolis, MN, USA).

Techniques: In Vitro, Expressing, Western Blot, Concentration Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay, Aortic Ring Assay

HIF-2 α activation by heparanase requires integrin β 1. (A) : Western blot and quantification of integrin β 1 and β 3 expressions in HUVECs treated with conditioned medium from MSC null , MSC hpa , and MSC hpa-KD . (B) : HIF-2 α and integrin β 1 expressions are decreased in integrin β 1 knockdown HUVECs. (C) : Representative images and bar graph of cell migration in HUVECs transfected with vector or integrin β 1 shRNA lentivirus; Bar = 50 μm, n = 3, ** denotes p < .01. (D) : Quantification of VEGF expression in MSC WT , MSC null , MSC hpa-KD , and MSC hpa . (E) : Quantification of VEGF, PDGF-BB, HGF, and TGF- β concentrations by ELISA in conditioned medium of MSC WT , MSC null , MSC hpa-KD , and MSC hpa ; n = 4 for each group. * denotes p < .05 (hpa vs. null) and ## denotes p < .01 (hpa-KD vs. null). (F) : Viability of MSCs in different concentrations of OGT2115 by CCK-8 assay. (G) : Bar graph shows quantitative analysis of cell migration, respectively; n = 3 for each group. * denotes p < .05 (hpa vs. null), ## denotes p < .01 (hpa1OGT2115 vs. hpa). (H) : Detection of heparanase concentration by ELISA in conditioned medium of MSC WT , MSC null , MSC hpa , and MSC hpa + OGT2115; n = 4 for each group, * denotes p < .05 (hpa vs. null), # denotes p < .05 (hpa+OGT2115 vs. hpa). (I) : Detection of VEGF, PDGF-BB, HGF, and TGF- β concentrations by ELISA in conditioned medium of MSC hpa and MSC hpa with OGT2115, n = 4 for each group. (J, K) : Bar graph shows quantitative analysis of cell migration and tube formation in each group; n = 3, ** denotes p < .01, * denotes p < .05. (L) : Quantification of integrin β 1, Flk-1, and HIF-2 α expression in HUVECs after incubated with active heparanase; n = 3. Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; HUVEC, human umbilical vein endothelial cell; MSC, mesenchymal stem cell.

Journal: Stem cells (Dayton, Ohio)

Article Title: Heparanase Released From Mesenchymal Stem Cells Activates Integrin beta1/HIF-2alpha/Flk-1 Signaling and Promotes Endothelial Cell Migration and Angiogenesis

doi: 10.1002/stem.1995

Figure Lengend Snippet: HIF-2 α activation by heparanase requires integrin β 1. (A) : Western blot and quantification of integrin β 1 and β 3 expressions in HUVECs treated with conditioned medium from MSC null , MSC hpa , and MSC hpa-KD . (B) : HIF-2 α and integrin β 1 expressions are decreased in integrin β 1 knockdown HUVECs. (C) : Representative images and bar graph of cell migration in HUVECs transfected with vector or integrin β 1 shRNA lentivirus; Bar = 50 μm, n = 3, ** denotes p < .01. (D) : Quantification of VEGF expression in MSC WT , MSC null , MSC hpa-KD , and MSC hpa . (E) : Quantification of VEGF, PDGF-BB, HGF, and TGF- β concentrations by ELISA in conditioned medium of MSC WT , MSC null , MSC hpa-KD , and MSC hpa ; n = 4 for each group. * denotes p < .05 (hpa vs. null) and ## denotes p < .01 (hpa-KD vs. null). (F) : Viability of MSCs in different concentrations of OGT2115 by CCK-8 assay. (G) : Bar graph shows quantitative analysis of cell migration, respectively; n = 3 for each group. * denotes p < .05 (hpa vs. null), ## denotes p < .01 (hpa1OGT2115 vs. hpa). (H) : Detection of heparanase concentration by ELISA in conditioned medium of MSC WT , MSC null , MSC hpa , and MSC hpa + OGT2115; n = 4 for each group, * denotes p < .05 (hpa vs. null), # denotes p < .05 (hpa+OGT2115 vs. hpa). (I) : Detection of VEGF, PDGF-BB, HGF, and TGF- β concentrations by ELISA in conditioned medium of MSC hpa and MSC hpa with OGT2115, n = 4 for each group. (J, K) : Bar graph shows quantitative analysis of cell migration and tube formation in each group; n = 3, ** denotes p < .01, * denotes p < .05. (L) : Quantification of integrin β 1, Flk-1, and HIF-2 α expression in HUVECs after incubated with active heparanase; n = 3. Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; HUVEC, human umbilical vein endothelial cell; MSC, mesenchymal stem cell.

Article Snippet: The lower chamber was filled with MSC-conditioned medium or control medium as above or with recombinant human active heparanase (100 mg/ml, R&D Systems, Minneapolis, MN, USA).

Techniques: Activation Assay, Western Blot, Knockdown, Migration, Transfection, Plasmid Preparation, shRNA, Expressing, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Concentration Assay, Incubation, Modification

Schematic of cell migration and angiogenesis by MSC-secreted heparanase. Abbreviation: MSC, mesenchymal stem cell.

Journal: Stem cells (Dayton, Ohio)

Article Title: Heparanase Released From Mesenchymal Stem Cells Activates Integrin beta1/HIF-2alpha/Flk-1 Signaling and Promotes Endothelial Cell Migration and Angiogenesis

doi: 10.1002/stem.1995

Figure Lengend Snippet: Schematic of cell migration and angiogenesis by MSC-secreted heparanase. Abbreviation: MSC, mesenchymal stem cell.

Article Snippet: The lower chamber was filled with MSC-conditioned medium or control medium as above or with recombinant human active heparanase (100 mg/ml, R&D Systems, Minneapolis, MN, USA).

Techniques: Migration