activation kit Search Results


t cell expansion kit  (Miltenyi Biotec)
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Miltenyi Biotec t cell expansion kit
T Cell Expansion Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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laminin 1  (Danaher Inc)
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Laminin 1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chip it high sensitivity kit  (Active Motif)
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Chip It High Sensitivity Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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global dna methylation line 1 kit  (Active Motif)
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Active Ras Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase 3 activity assay kit  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc caspase 3 activity assay kit
Caspase 3 Activity Assay Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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senescence β galactosidase activity assay kit  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc senescence β galactosidase activity assay kit
Increased mitochondrial respiratory supercomplex assembly and impaired <t>senescence‐associated</t> phenotypes in male COX7RP ‐Tg mice. (a) Increased oxygen consumption rates (OCRs) in COX7RP ‐Tg mice. OCR was measured in WAT and quadriceps femoris muscles dissected from 2‐year‐old COX7RP ‐Tg and WT mice using a Flux analyzer with the sequential addition of oligomycin, carbonyl cyanide 4‐(trifluoromethoxy)phenylhydrazone (FCCP), and rotenone/antimycin A ( n = 6). Basal and maximum OCRs were calculated after subtracting non‐mitochondrial OCR and the data are shown as means ± SD in the right panel ( n = 6). (b and c) Increased ATP synthesis (b) and decreased ROS levels (c) in mitochondria from WAT and quadriceps femoris muscle in COX7RP ‐Tg mice compared with WT mice at 2 years old. Data are presented as means ± SD ( n = 3). (d) Mitochondrial respiratory supercomplex assembly is enhanced in WAT and quadriceps femoris muscle in COX7RP ‐Tg mice. Mitochondrial proteins prepared from 2‐year‐old COX7RP ‐Tg and WT mice were solubilized with digitonin (4 g g −1 ) and subjected to BN‐PAGE. Western blot analysis was performed with antibodies for COX1 and Fp70. Positions corresponding to CI+ CIII 2 + CIV n , CIII 2 + CIV, CIII 2 or CIV n , and CIV are indicated. (e) Cellular nicotinamide adenine dinucleotide (NAD + ) level is increased in WAT and quadriceps femoris muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). (f) <t>Senescence‐associated</t> <t>β‐galactosidase</t> (SA‐β‐gal) activity is decreased in WAT and gastrocnemius muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). Differences between COX7RP ‐Tg and WT mice were analyzed using a two‐tailed Student t ‐test. * p < 0.05; ** p < 0.01.
Senescence β Galactosidase Activity Assay Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase 8 activities  (Beyotime)
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Beyotime caspase 8 activities
Increased mitochondrial respiratory supercomplex assembly and impaired <t>senescence‐associated</t> phenotypes in male COX7RP ‐Tg mice. (a) Increased oxygen consumption rates (OCRs) in COX7RP ‐Tg mice. OCR was measured in WAT and quadriceps femoris muscles dissected from 2‐year‐old COX7RP ‐Tg and WT mice using a Flux analyzer with the sequential addition of oligomycin, carbonyl cyanide 4‐(trifluoromethoxy)phenylhydrazone (FCCP), and rotenone/antimycin A ( n = 6). Basal and maximum OCRs were calculated after subtracting non‐mitochondrial OCR and the data are shown as means ± SD in the right panel ( n = 6). (b and c) Increased ATP synthesis (b) and decreased ROS levels (c) in mitochondria from WAT and quadriceps femoris muscle in COX7RP ‐Tg mice compared with WT mice at 2 years old. Data are presented as means ± SD ( n = 3). (d) Mitochondrial respiratory supercomplex assembly is enhanced in WAT and quadriceps femoris muscle in COX7RP ‐Tg mice. Mitochondrial proteins prepared from 2‐year‐old COX7RP ‐Tg and WT mice were solubilized with digitonin (4 g g −1 ) and subjected to BN‐PAGE. Western blot analysis was performed with antibodies for COX1 and Fp70. Positions corresponding to CI+ CIII 2 + CIV n , CIII 2 + CIV, CIII 2 or CIV n , and CIV are indicated. (e) Cellular nicotinamide adenine dinucleotide (NAD + ) level is increased in WAT and quadriceps femoris muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). (f) <t>Senescence‐associated</t> <t>β‐galactosidase</t> (SA‐β‐gal) activity is decreased in WAT and gastrocnemius muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). Differences between COX7RP ‐Tg and WT mice were analyzed using a two‐tailed Student t ‐test. * p < 0.05; ** p < 0.01.
Caspase 8 Activities, supplied by Beyotime, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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activity kits  (Beyotime)
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Beyotime activity kits
Increased mitochondrial respiratory supercomplex assembly and impaired <t>senescence‐associated</t> phenotypes in male COX7RP ‐Tg mice. (a) Increased oxygen consumption rates (OCRs) in COX7RP ‐Tg mice. OCR was measured in WAT and quadriceps femoris muscles dissected from 2‐year‐old COX7RP ‐Tg and WT mice using a Flux analyzer with the sequential addition of oligomycin, carbonyl cyanide 4‐(trifluoromethoxy)phenylhydrazone (FCCP), and rotenone/antimycin A ( n = 6). Basal and maximum OCRs were calculated after subtracting non‐mitochondrial OCR and the data are shown as means ± SD in the right panel ( n = 6). (b and c) Increased ATP synthesis (b) and decreased ROS levels (c) in mitochondria from WAT and quadriceps femoris muscle in COX7RP ‐Tg mice compared with WT mice at 2 years old. Data are presented as means ± SD ( n = 3). (d) Mitochondrial respiratory supercomplex assembly is enhanced in WAT and quadriceps femoris muscle in COX7RP ‐Tg mice. Mitochondrial proteins prepared from 2‐year‐old COX7RP ‐Tg and WT mice were solubilized with digitonin (4 g g −1 ) and subjected to BN‐PAGE. Western blot analysis was performed with antibodies for COX1 and Fp70. Positions corresponding to CI+ CIII 2 + CIV n , CIII 2 + CIV, CIII 2 or CIV n , and CIV are indicated. (e) Cellular nicotinamide adenine dinucleotide (NAD + ) level is increased in WAT and quadriceps femoris muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). (f) <t>Senescence‐associated</t> <t>β‐galactosidase</t> (SA‐β‐gal) activity is decreased in WAT and gastrocnemius muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). Differences between COX7RP ‐Tg and WT mice were analyzed using a two‐tailed Student t ‐test. * p < 0.05; ** p < 0.01.
Activity Kits, supplied by Beyotime, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase 1 activity assay caspase 1 activity  (Beyotime)
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Beyotime caspase 1 activity assay caspase 1 activity
Increased mitochondrial respiratory supercomplex assembly and impaired <t>senescence‐associated</t> phenotypes in male COX7RP ‐Tg mice. (a) Increased oxygen consumption rates (OCRs) in COX7RP ‐Tg mice. OCR was measured in WAT and quadriceps femoris muscles dissected from 2‐year‐old COX7RP ‐Tg and WT mice using a Flux analyzer with the sequential addition of oligomycin, carbonyl cyanide 4‐(trifluoromethoxy)phenylhydrazone (FCCP), and rotenone/antimycin A ( n = 6). Basal and maximum OCRs were calculated after subtracting non‐mitochondrial OCR and the data are shown as means ± SD in the right panel ( n = 6). (b and c) Increased ATP synthesis (b) and decreased ROS levels (c) in mitochondria from WAT and quadriceps femoris muscle in COX7RP ‐Tg mice compared with WT mice at 2 years old. Data are presented as means ± SD ( n = 3). (d) Mitochondrial respiratory supercomplex assembly is enhanced in WAT and quadriceps femoris muscle in COX7RP ‐Tg mice. Mitochondrial proteins prepared from 2‐year‐old COX7RP ‐Tg and WT mice were solubilized with digitonin (4 g g −1 ) and subjected to BN‐PAGE. Western blot analysis was performed with antibodies for COX1 and Fp70. Positions corresponding to CI+ CIII 2 + CIV n , CIII 2 + CIV, CIII 2 or CIV n , and CIV are indicated. (e) Cellular nicotinamide adenine dinucleotide (NAD + ) level is increased in WAT and quadriceps femoris muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). (f) <t>Senescence‐associated</t> <t>β‐galactosidase</t> (SA‐β‐gal) activity is decreased in WAT and gastrocnemius muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). Differences between COX7RP ‐Tg and WT mice were analyzed using a two‐tailed Student t ‐test. * p < 0.05; ** p < 0.01.
Caspase 1 Activity Assay Caspase 1 Activity, supplied by Beyotime, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase activity detection reagent kit  (Beyotime)
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Increased mitochondrial respiratory supercomplex assembly and impaired <t>senescence‐associated</t> phenotypes in male COX7RP ‐Tg mice. (a) Increased oxygen consumption rates (OCRs) in COX7RP ‐Tg mice. OCR was measured in WAT and quadriceps femoris muscles dissected from 2‐year‐old COX7RP ‐Tg and WT mice using a Flux analyzer with the sequential addition of oligomycin, carbonyl cyanide 4‐(trifluoromethoxy)phenylhydrazone (FCCP), and rotenone/antimycin A ( n = 6). Basal and maximum OCRs were calculated after subtracting non‐mitochondrial OCR and the data are shown as means ± SD in the right panel ( n = 6). (b and c) Increased ATP synthesis (b) and decreased ROS levels (c) in mitochondria from WAT and quadriceps femoris muscle in COX7RP ‐Tg mice compared with WT mice at 2 years old. Data are presented as means ± SD ( n = 3). (d) Mitochondrial respiratory supercomplex assembly is enhanced in WAT and quadriceps femoris muscle in COX7RP ‐Tg mice. Mitochondrial proteins prepared from 2‐year‐old COX7RP ‐Tg and WT mice were solubilized with digitonin (4 g g −1 ) and subjected to BN‐PAGE. Western blot analysis was performed with antibodies for COX1 and Fp70. Positions corresponding to CI+ CIII 2 + CIV n , CIII 2 + CIV, CIII 2 or CIV n , and CIV are indicated. (e) Cellular nicotinamide adenine dinucleotide (NAD + ) level is increased in WAT and quadriceps femoris muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). (f) <t>Senescence‐associated</t> <t>β‐galactosidase</t> (SA‐β‐gal) activity is decreased in WAT and gastrocnemius muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). Differences between COX7RP ‐Tg and WT mice were analyzed using a two‐tailed Student t ‐test. * p < 0.05; ** p < 0.01.
Caspase Activity Detection Reagent Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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activity assay kit  (Beyotime)
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Beyotime activity assay kit
Increased mitochondrial respiratory supercomplex assembly and impaired <t>senescence‐associated</t> phenotypes in male COX7RP ‐Tg mice. (a) Increased oxygen consumption rates (OCRs) in COX7RP ‐Tg mice. OCR was measured in WAT and quadriceps femoris muscles dissected from 2‐year‐old COX7RP ‐Tg and WT mice using a Flux analyzer with the sequential addition of oligomycin, carbonyl cyanide 4‐(trifluoromethoxy)phenylhydrazone (FCCP), and rotenone/antimycin A ( n = 6). Basal and maximum OCRs were calculated after subtracting non‐mitochondrial OCR and the data are shown as means ± SD in the right panel ( n = 6). (b and c) Increased ATP synthesis (b) and decreased ROS levels (c) in mitochondria from WAT and quadriceps femoris muscle in COX7RP ‐Tg mice compared with WT mice at 2 years old. Data are presented as means ± SD ( n = 3). (d) Mitochondrial respiratory supercomplex assembly is enhanced in WAT and quadriceps femoris muscle in COX7RP ‐Tg mice. Mitochondrial proteins prepared from 2‐year‐old COX7RP ‐Tg and WT mice were solubilized with digitonin (4 g g −1 ) and subjected to BN‐PAGE. Western blot analysis was performed with antibodies for COX1 and Fp70. Positions corresponding to CI+ CIII 2 + CIV n , CIII 2 + CIV, CIII 2 or CIV n , and CIV are indicated. (e) Cellular nicotinamide adenine dinucleotide (NAD + ) level is increased in WAT and quadriceps femoris muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). (f) <t>Senescence‐associated</t> <t>β‐galactosidase</t> (SA‐β‐gal) activity is decreased in WAT and gastrocnemius muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). Differences between COX7RP ‐Tg and WT mice were analyzed using a two‐tailed Student t ‐test. * p < 0.05; ** p < 0.01.
Activity Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Increased mitochondrial respiratory supercomplex assembly and impaired senescence‐associated phenotypes in male COX7RP ‐Tg mice. (a) Increased oxygen consumption rates (OCRs) in COX7RP ‐Tg mice. OCR was measured in WAT and quadriceps femoris muscles dissected from 2‐year‐old COX7RP ‐Tg and WT mice using a Flux analyzer with the sequential addition of oligomycin, carbonyl cyanide 4‐(trifluoromethoxy)phenylhydrazone (FCCP), and rotenone/antimycin A ( n = 6). Basal and maximum OCRs were calculated after subtracting non‐mitochondrial OCR and the data are shown as means ± SD in the right panel ( n = 6). (b and c) Increased ATP synthesis (b) and decreased ROS levels (c) in mitochondria from WAT and quadriceps femoris muscle in COX7RP ‐Tg mice compared with WT mice at 2 years old. Data are presented as means ± SD ( n = 3). (d) Mitochondrial respiratory supercomplex assembly is enhanced in WAT and quadriceps femoris muscle in COX7RP ‐Tg mice. Mitochondrial proteins prepared from 2‐year‐old COX7RP ‐Tg and WT mice were solubilized with digitonin (4 g g −1 ) and subjected to BN‐PAGE. Western blot analysis was performed with antibodies for COX1 and Fp70. Positions corresponding to CI+ CIII 2 + CIV n , CIII 2 + CIV, CIII 2 or CIV n , and CIV are indicated. (e) Cellular nicotinamide adenine dinucleotide (NAD + ) level is increased in WAT and quadriceps femoris muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). (f) Senescence‐associated β‐galactosidase (SA‐β‐gal) activity is decreased in WAT and gastrocnemius muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). Differences between COX7RP ‐Tg and WT mice were analyzed using a two‐tailed Student t ‐test. * p < 0.05; ** p < 0.01.

Journal: Aging Cell

Article Title: Mitochondrial Respiratory Supercomplex Assembly Factor COX7RP Contributes to Lifespan Extension in Mice

doi: 10.1111/acel.70294

Figure Lengend Snippet: Increased mitochondrial respiratory supercomplex assembly and impaired senescence‐associated phenotypes in male COX7RP ‐Tg mice. (a) Increased oxygen consumption rates (OCRs) in COX7RP ‐Tg mice. OCR was measured in WAT and quadriceps femoris muscles dissected from 2‐year‐old COX7RP ‐Tg and WT mice using a Flux analyzer with the sequential addition of oligomycin, carbonyl cyanide 4‐(trifluoromethoxy)phenylhydrazone (FCCP), and rotenone/antimycin A ( n = 6). Basal and maximum OCRs were calculated after subtracting non‐mitochondrial OCR and the data are shown as means ± SD in the right panel ( n = 6). (b and c) Increased ATP synthesis (b) and decreased ROS levels (c) in mitochondria from WAT and quadriceps femoris muscle in COX7RP ‐Tg mice compared with WT mice at 2 years old. Data are presented as means ± SD ( n = 3). (d) Mitochondrial respiratory supercomplex assembly is enhanced in WAT and quadriceps femoris muscle in COX7RP ‐Tg mice. Mitochondrial proteins prepared from 2‐year‐old COX7RP ‐Tg and WT mice were solubilized with digitonin (4 g g −1 ) and subjected to BN‐PAGE. Western blot analysis was performed with antibodies for COX1 and Fp70. Positions corresponding to CI+ CIII 2 + CIV n , CIII 2 + CIV, CIII 2 or CIV n , and CIV are indicated. (e) Cellular nicotinamide adenine dinucleotide (NAD + ) level is increased in WAT and quadriceps femoris muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). (f) Senescence‐associated β‐galactosidase (SA‐β‐gal) activity is decreased in WAT and gastrocnemius muscle of COX7RP ‐Tg mice at 2 years old. Data are presented as means ± SD ( n = 3). Differences between COX7RP ‐Tg and WT mice were analyzed using a two‐tailed Student t ‐test. * p < 0.05; ** p < 0.01.

Article Snippet: SA‐β‐gal activity was measured using the Senescence β‐galactosidase Activity Assay Kit (Cell Signaling Technology) according to the manufacturer's instructions.

Techniques: Muscles, Western Blot, Activity Assay, Two Tailed Test