Journal: EMBO Molecular Medicine

Article Title: Characterization and therapy of fertilization failure in murine and human models with HNRNPR mutations

doi: 10.1038/s44321-026-00374-z

Figure Lengend Snippet: ( A ) MII oocyte and zygote fluorescence imaging reflecting fertilization after ICSI of Hnrnpr -mutated sperm. Formation of male and female pronuclei indicates successful fertilization. Tubulin labels the spindle, showing that the oocyte is at metaphase of meiosis II, and P1 marks the first polar body. The white dotted line defines the boundary of the zygotes. Scale bar = 10 μm. ( B ) Quantification of the percentage of zygotes from ( A ). Data are presented as mean ± s.d., and P values were calculated using a two-sided Student’s t test. Data in ( A , B ) represent results from six independent experiments (two technical and three biological replicates, n = 6).

Article Snippet: Rabbit anti-alpha Tubulin , MedChemExpress , Cat# HY- P86200.

Techniques: Fluorescence, Imaging

Journal: The Journal of international medical research

Article Title: Physical properties of poly(N-isopropylacrylamide) hydrogel promote its effects on cardiac protection after myocardial infarction.

doi: 10.1177/030006051204000615

Figure Lengend Snippet: FIGURE 6: Neovasculature formation in sham-operated and infarct areas of myocardium 90 days after induction of myocardial infarction (MI) in rats, as identified by immunohistochemical staining for α-smooth muscle actin. (A) Sham-operated group; (B) phosphate-buffered saline (PBS)-treated MI group; (C) Gel A-treated MI group; (D) Gel B-treated MI group (scale bars, 100 µm). Immunohistochemical staining was used to quantify (E) blood vessel density per high-power field (HPF) in each group. Black arrows show neovasculature formed in sham-operated or infarcted myocardium. aP < 0.05 compared with sham-operated group; bP < 0.05 compared with MI + PBS group; one-way analysis of variance and unpaired Student’s t-test

Article Snippet: The slides were incubated overnight at 4 °C with anti-αsmooth muscle actin primary antibody (dilution 1 : 50; Boster, Wuhan, China).

Techniques: Immunohistochemical staining, Staining, Saline

Journal: Journal of thoracic disease

Article Title: Deletion of ACTA2 in mice promotes angiotensin II induced pathogenesis of thoracic aortic aneurysms and dissections.

doi: 10.21037/jtd.2018.07.75

Figure Lengend Snippet: Figure 2 Increased OPN expression and decreased α-SMA expression in AngII-treated ACTA2

Article Snippet: A mouse germline ACTA2 mutation was generated in a mixed C57BL/6J background in Cyagen Biosciences of Guangdong.

Techniques: Expressing

Journal: Journal of thoracic disease

Article Title: Deletion of ACTA2 in mice promotes angiotensin II induced pathogenesis of thoracic aortic aneurysms and dissections.

doi: 10.21037/jtd.2018.07.75

Figure Lengend Snippet: Figure 4 ACTA2 deficiency rendered aortic SMC susceptible to apoptosis. The expression levels of Bax and Bcl-2 were assessed by western

Article Snippet: A mouse germline ACTA2 mutation was generated in a mixed C57BL/6J background in Cyagen Biosciences of Guangdong.

Techniques: Expressing, Western Blot

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Dysregulated cholesterol uptake and efflux of bone marrow-derived α-SMA + macrophages contribute to atherosclerotic plaque formation

doi: 10.1007/s00018-025-05655-3

Figure Lengend Snippet: Macrophage-specific deletion of α-SMA inhibits atherosclerotic plaque formation. A Strategy for the generation of Acta2 f/f mice. B Genotyping results for flox1 (F1), flox2 (F2), or LysM-Cre. C Acta2 mRNA levels in BMDMs from Acta2 f/f or Acta2 MKO mice. D Schematic representation of the experimental workflow. E Representative en face images of Sudan IV-stained aortas from Acta2 f/f or Acta2 MKO mice treated with AAV -PCSK9 DY followed by a 12-week HFD. The mean aortic lesion area was quantified. F – H Representative Oil Red O-stained aortic root sections F , with quantification of lesion size G–H . Scale bar = 200 μm. n = 7 or 8. ** P < 0.01 by unpaired Student’s t-test. I , J Representative immunofluorescence staining for macrophages (MOMA-2, I ) and smooth muscle cells (α-SMA, J ) in the aortic root after a 12-week HFD. Purple, MOMA-2; red, α-SMA; blue, DAPI. Black scale bar = 200 μm, white scale bar = 50 μm. n = 7. * P < 0.05 by unpaired Student’s t-test. AAV, adeno-associated virus; HFD, high-fat diet

Article Snippet: Acta2 f/f (C57BL/6J background) mice were generated by Cyagen Biosciences (Guangzhou, China).

Techniques: Staining, Immunofluorescence, Virus

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Dysregulated cholesterol uptake and efflux of bone marrow-derived α-SMA + macrophages contribute to atherosclerotic plaque formation

doi: 10.1007/s00018-025-05655-3

Figure Lengend Snippet: α-SMA promotes the lipid uptake and inhibits lipid efflux in macrophages. A , B Flow cytometric analysis of binding and uptake of DiI-Ox-LDL in vector- or Acta2 hi RAW264.7 cells A , and BMDMs from Acta2 f/f or Acta2 MKO mice B . The quantification results are shown on the right. n = 3. * P < 0.05, *** P < 0.001, **** P < 0.0001 by unpaired Student’s t-test. C , D SR-A, ABCA1, ABCG1 and α-SMA protein levels in vector- and Acta2 hi RAW264.7 cells C , and BMDMs from Acta2 Flox or Acta2 MKO mice D . n = 3. * P < 0.05 by unpaired Student’s t-test. E–F Co-staining and quantification of SR-A + MOMA-2 + E or ABCA1 + MOMA-2 + F cells in aortic roots from Acta2 f/f or Acta2 MKO mice treated with AAV -PCSK9 DY followed by a 12-week HFD. n = 7. * P < 0.05 by unpaired Student’s t-test. G–H Flow cytometric analysis of binding G and uptake H of DiI-Ox-LDL in Acta2 hi RAW264.7 cells treated with an SR-A blocking antibody (20 μg/mL) or IgG control for 2 h were detected by flow cytometry. n = 3. * P < 0.05 by unpaired Student’s t-test. AAV, adeno-associated virus; HFD, high-fat diet

Article Snippet: Acta2 f/f (C57BL/6J background) mice were generated by Cyagen Biosciences (Guangzhou, China).

Techniques: Binding Assay, Plasmid Preparation, Staining, Blocking Assay, Control, Flow Cytometry, Virus

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Dysregulated cholesterol uptake and efflux of bone marrow-derived α-SMA + macrophages contribute to atherosclerotic plaque formation

doi: 10.1007/s00018-025-05655-3

Figure Lengend Snippet: AKT pathway is involved in α-SMA-induced lipid accumulation. A Abca1 mRNA levels in vector or Acta2 hi RAW264.7 cells treated with 50 μg/ml Ox-LDL for 0, 6, 12, or 24 h. n = 3. * P < 0.05 by unpaired Student’s t-test. B Top 15 KEGG pathways of genes downregulated in Acta2 hi RAW264.7 cells incubated with Ox-LDL for 6 h. C Protein levels of p-AKT, AKT, p-ERK, ERK, p-STAT3, STAT3, CD36, SR-A, ABCA1 and α-SMA treated with 50 μg/ml Ox-LDL for 6 h. n = 3. * P < 0.05 by one-way ANOVA. D – F Flow cytometric analysis of binding ( D and E ) and uptake ( D and F ) of DiI-Ox-LDL in vector or Acta2 hi RAW264.7 cells incubated with or without SC79 (2 μg/mL). n = 3. * P < 0.05, ** P < 0.01, **** P < 0.0001 by one-way ANOVA. G – I Protein levels of CD36, SR-A, ABCA1 and α-SMA in Acta2 hi or vector RAW264.7 cells incubated with or without SC79 (2 μg/mL) and treated with 50 μg/ml Ox-LDL for 2 h. n = 3. * P < 0.05, ** P < 0.01, **** P < 0.0001 by one-way ANOVA

Article Snippet: Acta2 f/f (C57BL/6J background) mice were generated by Cyagen Biosciences (Guangzhou, China).

Techniques: Plasmid Preparation, Incubation, Binding Assay