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Image Search Results
Journal: Cell reports
Article Title: Chronic hypoxia stabilizes 3βHSD1 via autophagy suppression
doi: 10.1016/j.celrep.2023.113575
Figure Lengend Snippet: (A and B) HR LNCaP and C4-2 cells were treated with acetate (5 mM) for 24 h under hypoxia. (A) H3K9ac, H3K27ac, and H3 proteins were determined by western blot. H3 was the loading control. (B) BECN1, ATG10, ATG7, ATG3, ATG4A, ATG4B, LC3B , and ATG2A mRNA was determined by qPCR. ACTB was the loading control. (C and D) Both ACSS1 and ACSS2 were stably knocked down by shRNA in HR cells. (C) ACSS1, ACSS2, H3K9ac, H3K27ac, and H3 proteins were determined by Western blot. H3 was the loading control. (D) ACSS1, ACSS2, BECN1, ATG10, ATG7, ATG3, ATG4A, ATG4B, LC3B , and ATG2A mRNA was determined by qPCR. (E and F) ACSS1 or ACSS2 was overexpressed in HR cells. (E) ACSS1, ACSS2, H3K9ac, H3K27ac, and H3 proteins were determined by western blot. H3 was the loading control. (F) BECN1, ATG10, ATG7, ATG3, ATG4A, ATG4B, LC3B , and ATG2A mRNA was determined by qPCR. ACTB was the loading control. *p < 0.05 using a one sample two-tailed t test. Error bars represent mean ± SEM.
Article Snippet: The following plasmids were purchased from
Techniques: Western Blot, Stable Transfection, shRNA, Two Tailed Test
Journal: Cell reports
Article Title: Chronic hypoxia stabilizes 3βHSD1 via autophagy suppression
doi: 10.1016/j.celrep.2023.113575
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: The following plasmids were purchased from
Techniques: Recombinant, Transfection, Mutagenesis, Viability Assay, Sequencing, Negative Control, shRNA, Plasmid Preparation, Expressing, Variant Assay, Software
Journal: Clinical, Cosmetic and Investigational Dermatology
Article Title: Biguanides Induce Acute de novo Lipogenesis in Human Primary Sebocytes
doi: 10.2147/CCID.S243154
Figure Lengend Snippet: Gene Expression Changes in Primary Sebocytes After 48 hrs
Article Snippet: Reverse transcription reactions were prepared using 1 μg of total RNA and qPCR analysis was performed in duplicate using 10 ng/well cDNA to determine the transcript levels of SREBF1 (Hs01088691_m1), FASN (Hs01005622_m1), ACSS2 (
Techniques: Gene Expression