Journal: Journal of Lipid Research

Article Title: Dyslipidemia-induced renal fibrosis related to ferroptosis and endoplasmic reticulum stress

doi: 10.1016/j.jlr.2024.100610

Figure Lengend Snippet: High-fat diet induced ferroptosis in the kidney. A, B: Representative C11-BODIPY 581/591 staining showed increased renal lipid peroxidation in the two HFD groups. C: Malondialdehyde (MDA). D: Ferrous ions increased in WT-HFD. E, F: Ferroptosis markers ( Gpx4 and Acsl4 ) mRNA expression in kidney tissue. Scale bar = 20 μm. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 between groups. Acsl4 , acyl-CoA synthetase long-chain family member-4; Gpx4 , glutathione peroxidase-4; WT, wild-type.

Article Snippet: To detect ferroptosis mediators, mRNA expression of Gpx4 (Ss03384646, Thermo Fisher Scientific) and Acsl4 (Ss03379974) was studied using quantitative real-time PCR in kidney tissues ( ).

Techniques: Staining, Expressing

Journal: International journal of molecular sciences

Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import.

doi: 10.3390/ijms251910832

Figure Lengend Snippet: Figure 1. (A) Protein levels of MPV17 in cardiomyocytes treated with FAC evaluated by WB; (B) the overexpression of MPV17 in cardiomyocytes was detected by WB; (C) cell death labeled with PI staining in each group; (D) the knockdown of MPV17 in cardiomyocytes by siRNA was detected by WB; (E) cell death labeled with PI staining in each group; (F) C11-BO was applied to label the lipid peroxides and flow cytometry was used for the detection of green fluoresce in each group; (G) C11-BO was applied to label the lipid peroxides and flow cytometry was used for the detection of green fluoresce in each group; (H) ACSL4, FTH–1 and GPX–4 were evaluated by WB; (I) ACSL4, FTH–1 and GPX–4 were evaluated by WB; *, p < 0.05 was statistically significant.

Article Snippet: The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).

Techniques: Over Expression, Labeling, Staining, Knockdown, Flow Cytometry

Journal: International journal of molecular sciences

Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import.

doi: 10.3390/ijms251910832

Figure Lengend Snippet: Figure 2. (A) Prussion Blue staining for the examination of iron levels in cardiac tissues; scale bar, 60 µm; (B) cell death labeled with PI staining in cardiac tissues after I/R surgery; red, PI; blue, DAPI; green, Actinin; scale bar, 60 µm; (C) statistic data of the relative MDA levels; (D) the expression of ACSL4, FTH–1 and GPX–4 were checked by WB; (E) cell death labeled with PI staining in cardiac tissues after I/R surgery; red, PI; blue, DAPI; green, Actinin; scale bar, 60 µm; (F) Masson trichrome staining for the examination of collagen in the heart tissue; (G) LVIDd, diastolic left ventricular internal diameter; (H) EF, the ejection fraction of the left ventricular diameter; FS, the fractional shortening of the left ventricular diameter; (I) statistic data of the relative MDA levels in each group; (J) the expression of MPV17, ACSL4, FTH–1 and GPX–4 were checked by WB; *, p < 0.05 was statistically significant; ns, none significance.

Article Snippet: The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).

Techniques: Staining, Labeling, Expressing