Journal: Scientific Reports

Article Title: Three dimensional modeling of biologically relevant fluid shear stress in human renal tubule cells mimics in vivo transcriptional profiles

doi: 10.1038/s41598-021-93570-5

Figure Lengend Snippet: Analysis of human proximal tubular albumin reuptake function. Albumin uptake by human proximal tubular epithelial cells after 15 min incubation at 37 °C with 50 µg mL-1 of FITC-albumin (green) added to the channel under static conditions ( A ) and fluid shear stress conditions ( B ). Mean Fluorescence Intensity (MFI) of RPTEC/TERT1 cells 24 h after static conditions versus fluid shear stress conditions showing a significant increase in FITC-Albumin fluorescence signal ( C ) (***p ≤ 0.001). Transcriptome profiling provides insight to differentially expressed genes corresponding to endocytosis process.

Article Snippet: Human immortalized hRPTECs (RPTEC/TERT1, ATCC CRL-4031) and were cultured and maintained in hTERT Immortalized RPTEC Growth Kit (ATCC ACS-4007), supplemented with Geneticin (Gibco, 10131035), in phenol red free DMEM/F-12 medium (Gibco, 11039021) according to the vendor's instructions.

Techniques: Incubation, Shear, Fluorescence

Journal: Scientific Reports

Article Title: Three dimensional modeling of biologically relevant fluid shear stress in human renal tubule cells mimics in vivo transcriptional profiles

doi: 10.1038/s41598-021-93570-5

Figure Lengend Snippet: Fluorescent transporter substrate Calcein-AM and CMFDA amasses in RPTEC/TERT1 cells under static conditions and dissipates with the application of FSS. Figure ( B ) and ( D ) sample fluorescence images demonstrate decreased in Calcein-AM and CMFDA accumulation in cells after 24 h of FSS treatment prior to staining compared to static channels ( A and C ) (Bar = 100 µm). ( E ) Quantitative analysis of Mean Fluorescence Intensity (MFI) of Calcein-AM showed a significant decrease after cells were treated with 24 h of FSS versus static environment (yellow filled bars). Indicating the increased efflux activity after being kept under fluidic conditions (Bar = 100 µm; n = 4, ***p ≤ 0.001).

Article Snippet: Human immortalized hRPTECs (RPTEC/TERT1, ATCC CRL-4031) and were cultured and maintained in hTERT Immortalized RPTEC Growth Kit (ATCC ACS-4007), supplemented with Geneticin (Gibco, 10131035), in phenol red free DMEM/F-12 medium (Gibco, 11039021) according to the vendor's instructions.

Techniques: Fluorescence, Staining, Activity Assay

Journal: Cells

Article Title: FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla Cells.

doi: 10.3390/cells11172730

Figure Lengend Snippet: Figure 1. Differentiation of pluripotent stem cells into dermal papilla cells.

Article Snippet: Dermal papilla cells (iDP) differentiated from human pluripotent stem cells (iPSKYOU (ACS-1023TM, ATCC, USA), iPS-DYP0730 (ACS-1023TM, ATCC, USA), iPSC-DP [21], hES-MK05 (Vavilov Institute of General Genetics, Russian Academy of Sciences) and primary human dermal papilla cells (hDP) from 3 donors were used to create 3D dermal cultures.

Techniques: