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  • 85
    Waters Corporation acquity 1
    Acquity 1, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation acquity 1 7 um c 18 reverse phase column
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    Waters Corporation uplc hss t3 column
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    Waters Corporation uplc
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    Waters Corporation column c18
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    Waters Corporation c18 phase reverse column
    Isolation of CoaPLA 2 from Crotalus oreganus abyssus venom. (a) Profile obtained by gel chromatography on a G75-Sephadex column. Fraction II, presenting phospholipase activity. (b) Lyophilized fraction II was homogenized and centrifuged and subjected to reverse phase HPLC using an analytical <t>C18</t> column. (c) Assessment of purity of RP-HPLC under the same conditions used in (b). Electrophoretic analysis shows the homogeneity of the CoaPLA 2 isolated from the venom of Crotalus Oreganus abyssus : Lines 1 and 4: standard molecular weight; Lines 2 and 5: PAGE under nonreduced conditions; Lines 3 and 6: PAGE under reduced conditions (SDS-PAGE).
    C18 Phase Reverse Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation in line c18 uplc column
    Isolation of CoaPLA 2 from Crotalus oreganus abyssus venom. (a) Profile obtained by gel chromatography on a G75-Sephadex column. Fraction II, presenting phospholipase activity. (b) Lyophilized fraction II was homogenized and centrifuged and subjected to reverse phase HPLC using an analytical <t>C18</t> column. (c) Assessment of purity of RP-HPLC under the same conditions used in (b). Electrophoretic analysis shows the homogeneity of the CoaPLA 2 isolated from the venom of Crotalus Oreganus abyssus : Lines 1 and 4: standard molecular weight; Lines 2 and 5: PAGE under nonreduced conditions; Lines 3 and 6: PAGE under reduced conditions (SDS-PAGE).
    In Line C18 Uplc Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation c18 reverse phase capillary column
    Isolation of CoaPLA 2 from Crotalus oreganus abyssus venom. (a) Profile obtained by gel chromatography on a G75-Sephadex column. Fraction II, presenting phospholipase activity. (b) Lyophilized fraction II was homogenized and centrifuged and subjected to reverse phase HPLC using an analytical <t>C18</t> column. (c) Assessment of purity of RP-HPLC under the same conditions used in (b). Electrophoretic analysis shows the homogeneity of the CoaPLA 2 isolated from the venom of Crotalus Oreganus abyssus : Lines 1 and 4: standard molecular weight; Lines 2 and 5: PAGE under nonreduced conditions; Lines 3 and 6: PAGE under reduced conditions (SDS-PAGE).
    C18 Reverse Phase Capillary Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Isolation of CoaPLA 2 from Crotalus oreganus abyssus venom. (a) Profile obtained by gel chromatography on a G75-Sephadex column. Fraction II, presenting phospholipase activity. (b) Lyophilized fraction II was homogenized and centrifuged and subjected to reverse phase HPLC using an analytical C18 column. (c) Assessment of purity of RP-HPLC under the same conditions used in (b). Electrophoretic analysis shows the homogeneity of the CoaPLA 2 isolated from the venom of Crotalus Oreganus abyssus : Lines 1 and 4: standard molecular weight; Lines 2 and 5: PAGE under nonreduced conditions; Lines 3 and 6: PAGE under reduced conditions (SDS-PAGE).

    Journal: BioMed Research International

    Article Title: A Novel Phospholipase A2 (D49) from the Venom of the Crotalus oreganus abyssus (North American Grand Canyon Rattlesnake)

    doi: 10.1155/2014/654170

    Figure Lengend Snippet: Isolation of CoaPLA 2 from Crotalus oreganus abyssus venom. (a) Profile obtained by gel chromatography on a G75-Sephadex column. Fraction II, presenting phospholipase activity. (b) Lyophilized fraction II was homogenized and centrifuged and subjected to reverse phase HPLC using an analytical C18 column. (c) Assessment of purity of RP-HPLC under the same conditions used in (b). Electrophoretic analysis shows the homogeneity of the CoaPLA 2 isolated from the venom of Crotalus Oreganus abyssus : Lines 1 and 4: standard molecular weight; Lines 2 and 5: PAGE under nonreduced conditions; Lines 3 and 6: PAGE under reduced conditions (SDS-PAGE).

    Article Snippet: An aliquot of the tryptic digest was injected and separated in a C18 reverse phase column (75 μ m Oi, 10 cm, nano-Acquity, 1.7 μ m BEH column, Waters).

    Techniques: Isolation, Chromatography, Activity Assay, High Performance Liquid Chromatography, Molecular Weight, Polyacrylamide Gel Electrophoresis, SDS Page