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  • 94
    Waters Corporation acquity uplc system
    FKA concentrations in plasma and urine and its relationship with bladder weight of male UPII-SV40T transgenic mice A, Chromatographic separation of FKA was achieved using an <t>ACQUITY</t> <t>UPLC</t> (Waters, UK) with a reversed-phase ACQUITY UPLCTM BEP C18 column (1.7μm, 2.1× 50mm, Waters). FKA was monitored as a precursor ion with an m/z value of 315 and a fragment ion with a value at 181.14. A, a representative chromatograph of the plasma level of FKA after 0.6% dietary FKA feeding for 290 days in a male UPII-SV40T transgenic mouse. Flavokawain B (FKB) was used as an internal control. B, The mean FKA concentrations in plasma and urine in mice fed with vehicle control or 0.6% FKA for 290 days. Bar: mean ± SD. C, Correlation analysis between FKA plasma concentrations and bladder weights in male low copyUPII-SV40T transgenic mice. D, Correlation analysis between FKA plasma concentrations and bladder weights in female low copy UPII-SV40T transgenic mice.
    Acquity Uplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 11473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acquity uplc system/product/Waters Corporation
    Average 94 stars, based on 11473 article reviews
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    94
    waters corporation acquity uplc beh c18 column
    Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus <t>C18</t> column connected to a Xevo G2 XS equipped with an <t>ACQUITY</t> <t>UPLC</t> I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.
    Acquity Uplc Beh C18 Column, supplied by waters corporation, used in various techniques. Bioz Stars score: 94/100, based on 5367 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acquity uplc beh c18 column/product/waters corporation
    Average 94 stars, based on 5367 article reviews
    Price from $9.99 to $1999.99
    acquity uplc beh c18 column - by Bioz Stars, 2020-10
    94/100 stars
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    92
    Waters Corporation acquity ultra performance liquid chromatography uplc system
    Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus <t>C18</t> column connected to a Xevo G2 XS equipped with an <t>ACQUITY</t> <t>UPLC</t> I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.
    Acquity Ultra Performance Liquid Chromatography Uplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 810 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acquity ultra performance liquid chromatography uplc system/product/Waters Corporation
    Average 92 stars, based on 810 article reviews
    Price from $9.99 to $1999.99
    acquity ultra performance liquid chromatography uplc system - by Bioz Stars, 2020-10
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    91
    Waters Corporation nano acquity uplc system
    Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus <t>C18</t> column connected to a Xevo G2 XS equipped with an <t>ACQUITY</t> <t>UPLC</t> I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.
    Nano Acquity Uplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 914 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano acquity uplc system/product/Waters Corporation
    Average 91 stars, based on 914 article reviews
    Price from $9.99 to $1999.99
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    92
    Waters Corporation acquity ultra performance lc system
    Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus <t>C18</t> column connected to a Xevo G2 XS equipped with an <t>ACQUITY</t> <t>UPLC</t> I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.
    Acquity Ultra Performance Lc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 729 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acquity ultra performance lc system/product/Waters Corporation
    Average 92 stars, based on 729 article reviews
    Price from $9.99 to $1999.99
    acquity ultra performance lc system - by Bioz Stars, 2020-10
    92/100 stars
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    91
    Waters Corporation acquity ultra performance liquid chromatography system
    Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus <t>C18</t> column connected to a Xevo G2 XS equipped with an <t>ACQUITY</t> <t>UPLC</t> I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.
    Acquity Ultra Performance Liquid Chromatography System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acquity ultra performance liquid chromatography system/product/Waters Corporation
    Average 91 stars, based on 672 article reviews
    Price from $9.99 to $1999.99
    acquity ultra performance liquid chromatography system - by Bioz Stars, 2020-10
    91/100 stars
      Buy from Supplier

    90
    Waters Corporation acquity uhplc system
    Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus <t>C18</t> column connected to a Xevo G2 XS equipped with an <t>ACQUITY</t> <t>UPLC</t> I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.
    Acquity Uhplc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acquity uhplc system/product/Waters Corporation
    Average 90 stars, based on 420 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    FKA concentrations in plasma and urine and its relationship with bladder weight of male UPII-SV40T transgenic mice A, Chromatographic separation of FKA was achieved using an ACQUITY UPLC (Waters, UK) with a reversed-phase ACQUITY UPLCTM BEP C18 column (1.7μm, 2.1× 50mm, Waters). FKA was monitored as a precursor ion with an m/z value of 315 and a fragment ion with a value at 181.14. A, a representative chromatograph of the plasma level of FKA after 0.6% dietary FKA feeding for 290 days in a male UPII-SV40T transgenic mouse. Flavokawain B (FKB) was used as an internal control. B, The mean FKA concentrations in plasma and urine in mice fed with vehicle control or 0.6% FKA for 290 days. Bar: mean ± SD. C, Correlation analysis between FKA plasma concentrations and bladder weights in male low copyUPII-SV40T transgenic mice. D, Correlation analysis between FKA plasma concentrations and bladder weights in female low copy UPII-SV40T transgenic mice.

    Journal: Cancer prevention research (Philadelphia, Pa.)

    Article Title: KAVA chalcone, Flavokawain A, inhibits urothelial tumorigenesis in the UPII-SV40T transgenic mouse model

    doi: 10.1158/1940-6207.CAPR-13-0219

    Figure Lengend Snippet: FKA concentrations in plasma and urine and its relationship with bladder weight of male UPII-SV40T transgenic mice A, Chromatographic separation of FKA was achieved using an ACQUITY UPLC (Waters, UK) with a reversed-phase ACQUITY UPLCTM BEP C18 column (1.7μm, 2.1× 50mm, Waters). FKA was monitored as a precursor ion with an m/z value of 315 and a fragment ion with a value at 181.14. A, a representative chromatograph of the plasma level of FKA after 0.6% dietary FKA feeding for 290 days in a male UPII-SV40T transgenic mouse. Flavokawain B (FKB) was used as an internal control. B, The mean FKA concentrations in plasma and urine in mice fed with vehicle control or 0.6% FKA for 290 days. Bar: mean ± SD. C, Correlation analysis between FKA plasma concentrations and bladder weights in male low copyUPII-SV40T transgenic mice. D, Correlation analysis between FKA plasma concentrations and bladder weights in female low copy UPII-SV40T transgenic mice.

    Article Snippet: Chromatography was performed on an Acquity UPLC system (Waters Corp., Milford, MA, USA) with an auto-sampler at 8°C.

    Techniques: Transgenic Assay, Mouse Assay

    Example of a chromatographic separation of atracurium besylate stereoisomers using an Agilent Technologies Zorbax Eclipse XDB-C18 50 mm×4.6 mm column with 1.8 μm particle, using a potassium phosphate buffer–acetonitrile–methanol gradient at a flow rate of 1.0 mL/min and UV detection at 280 nm. Example of a chromatographic separation of a degraded solution of cis–cis atracurium besylate exposed to a temperature of 80 °C for 60 min. The same chromatographic conditions as those used in (a) were used. The peak eluting at 1.6 min corresponds to laudanosine (RRT=0.27); the peak eluting at 7.4 min corresponds to monoacrylate laudanosine besylate (RRT 1.26). Separation of a peptide from phenol preservative in a multi-dose formulation. Experimental conditions: Waters Acquity UPLC ® BEH C18 column (50 mm×2.1 mm, with 1.7 μm hybrid silica particles), using a mobile phase gradient with 0.1% aqueous phosphoric acid and acetonitrile in 4 min, at a flow rate of 0.6 mL/min, with an injection volume of 1 μL and detection wavelength at 220 nm. Solution concentration: 50 μg/mL.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Impurity profiling and in-process testing of drugs for injection by fast liquid chromatography

    doi: 10.1016/j.jpha.2012.07.004

    Figure Lengend Snippet: Example of a chromatographic separation of atracurium besylate stereoisomers using an Agilent Technologies Zorbax Eclipse XDB-C18 50 mm×4.6 mm column with 1.8 μm particle, using a potassium phosphate buffer–acetonitrile–methanol gradient at a flow rate of 1.0 mL/min and UV detection at 280 nm. Example of a chromatographic separation of a degraded solution of cis–cis atracurium besylate exposed to a temperature of 80 °C for 60 min. The same chromatographic conditions as those used in (a) were used. The peak eluting at 1.6 min corresponds to laudanosine (RRT=0.27); the peak eluting at 7.4 min corresponds to monoacrylate laudanosine besylate (RRT 1.26). Separation of a peptide from phenol preservative in a multi-dose formulation. Experimental conditions: Waters Acquity UPLC ® BEH C18 column (50 mm×2.1 mm, with 1.7 μm hybrid silica particles), using a mobile phase gradient with 0.1% aqueous phosphoric acid and acetonitrile in 4 min, at a flow rate of 0.6 mL/min, with an injection volume of 1 μL and detection wavelength at 220 nm. Solution concentration: 50 μg/mL.

    Article Snippet: 2 Materials and method The experimental results reported in this paper were obtained on Waters Acquity UPLC® (Milford, MA, USA) systems with either a Photodiode Array Detector or tunable UV (TUV) detector.

    Techniques: Flow Cytometry, Injection, Concentration Assay

    Example of chromatographic separation of a taxane from known impurities and degradants on a Waters Acquity UPLC ® BEH C18 column (100 mm×2.1 mm), using a water/acetonitrile gradient at a flow rate of 0.6 mL/min and UV detection at 228 nm.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Impurity profiling and in-process testing of drugs for injection by fast liquid chromatography

    doi: 10.1016/j.jpha.2012.07.004

    Figure Lengend Snippet: Example of chromatographic separation of a taxane from known impurities and degradants on a Waters Acquity UPLC ® BEH C18 column (100 mm×2.1 mm), using a water/acetonitrile gradient at a flow rate of 0.6 mL/min and UV detection at 228 nm.

    Article Snippet: 2 Materials and method The experimental results reported in this paper were obtained on Waters Acquity UPLC® (Milford, MA, USA) systems with either a Photodiode Array Detector or tunable UV (TUV) detector.

    Techniques: Flow Cytometry

    Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus C18 column connected to a Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.

    Journal: Molecules

    Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

    doi: 10.3390/molecules25112583

    Figure Lengend Snippet: Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus C18 column connected to a Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.

    Article Snippet: Liquid Chromatography-Mass Spectrometry (LC-MS) Extracted samples from the local source were redissolved in 10 mL methanol and aliquots analyzed with a qTof LC-MS using Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA, USA), or analyzed after pre-purification carried out using a 1260 Infinity II HPLC system with a C18 column (250 × 4.6 mm, 5 μm particle size) (Waters, Milford, MA, USA).

    Techniques:

    Purity and structure of the furanone, 5‐hydroxy‐3‐methoxy‐5‐methyl‐4‐butylfuran‐2(5H)‐one. Purity assay was carried out using a Waters Acquity H Class UPLC system with a PDA detector

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: The marine‐derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα, et al. The marine‐derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα

    doi: 10.1111/jcmm.15012

    Figure Lengend Snippet: Purity and structure of the furanone, 5‐hydroxy‐3‐methoxy‐5‐methyl‐4‐butylfuran‐2(5H)‐one. Purity assay was carried out using a Waters Acquity H Class UPLC system with a PDA detector

    Article Snippet: Ultra‐performance liquid chromatography (UPLC) spectrum was performed on an Acquity UPLC BEH C18 column (2.1 × 50 mm i.d., 1.7 μm) connected to a Waters Acquity H Class UPLC System (Waters) with a PDA detector (wavelength of 212 nm).

    Techniques:

    Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus C18 column connected to a Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.

    Journal: Molecules

    Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

    doi: 10.3390/molecules25112583

    Figure Lengend Snippet: Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus C18 column connected to a Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.

    Article Snippet: Chromatography for LC-MS was performed using a Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm) (Agilent Technology, Santa Clara, CA, USA), an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm), an Atlantis C18 column (100 × 4.6 mm, 5 μm) (Waters, Milford, MA, USA) or a Pursuit 200Å PFP column (4.6 × 150 mm, 5 µm) (Agilent Technology, Santa Clara, CA, USA).

    Techniques:

    Detection of 1,20(OH) 2 D3 in honey. ( A ) The peak corresponding in RT to standard was collected from commercially available honey using an Atlantis C18 column using a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 439.319 [M + Na] + . ( B ) The local honey sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

    Journal: Molecules

    Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

    doi: 10.3390/molecules25112583

    Figure Lengend Snippet: Detection of 1,20(OH) 2 D3 in honey. ( A ) The peak corresponding in RT to standard was collected from commercially available honey using an Atlantis C18 column using a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 439.319 [M + Na] + . ( B ) The local honey sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

    Article Snippet: Chromatography for LC-MS was performed using a Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm) (Agilent Technology, Santa Clara, CA, USA), an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm), an Atlantis C18 column (100 × 4.6 mm, 5 μm) (Waters, Milford, MA, USA) or a Pursuit 200Å PFP column (4.6 × 150 mm, 5 µm) (Agilent Technology, Santa Clara, CA, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Purification

    Detection of 20(OH)D3 and 20(OH)-7DHC in honey. ( A ) After a pre-purification step using an Atlantis C18 column with a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 423.324 [M + Na] + . ( B ) After extraction of commercially sourced honey, the sample was directly analyzed using LC-MS with a Pursuit 200Å PFP column with a methanol gradient. The EIC was obtained using m/z = 383.331 [M + H − H 2 O] + .

    Journal: Molecules

    Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

    doi: 10.3390/molecules25112583

    Figure Lengend Snippet: Detection of 20(OH)D3 and 20(OH)-7DHC in honey. ( A ) After a pre-purification step using an Atlantis C18 column with a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 423.324 [M + Na] + . ( B ) After extraction of commercially sourced honey, the sample was directly analyzed using LC-MS with a Pursuit 200Å PFP column with a methanol gradient. The EIC was obtained using m/z = 383.331 [M + H − H 2 O] + .

    Article Snippet: Chromatography for LC-MS was performed using a Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm) (Agilent Technology, Santa Clara, CA, USA), an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm), an Atlantis C18 column (100 × 4.6 mm, 5 μm) (Waters, Milford, MA, USA) or a Pursuit 200Å PFP column (4.6 × 150 mm, 5 µm) (Agilent Technology, Santa Clara, CA, USA).

    Techniques: Purification, Liquid Chromatography with Mass Spectroscopy

    Ion chromatograms of conjugated BAs on an ACQUITY BEH C18 column eluted by the Gradient-I with various buffers (from bottom to top: 0.01% formic acid, 2 mM ammonium formate at pH 3.5, 2 mM ammonium acetate at pH 4.5, 2 mM ammonium acetate at pH 5.5 and 2 mM ammonium bicarbonate at pH 6.5) and acetonitrile as the aqueous phase and the organic phase respectively.

    Journal: Analytical and bioanalytical chemistry

    Article Title: Factors affecting separation and detection of bile acids by liquid chromatography coupled with mass spectrometry at negative mode

    doi: 10.1007/s00216-017-0489-1

    Figure Lengend Snippet: Ion chromatograms of conjugated BAs on an ACQUITY BEH C18 column eluted by the Gradient-I with various buffers (from bottom to top: 0.01% formic acid, 2 mM ammonium formate at pH 3.5, 2 mM ammonium acetate at pH 4.5, 2 mM ammonium acetate at pH 5.5 and 2 mM ammonium bicarbonate at pH 6.5) and acetonitrile as the aqueous phase and the organic phase respectively.

    Article Snippet: The Gradient-I with a run time of 20 min utilized ACQUITY BEH C18 column (1.7 μm, 100 mm × 2.1 mm) (Waters, Milford, MA, USA) maintained at 45 °C.

    Techniques:

    Ion chromatograms of unconjugated BAs on an ACQUITY BEH C18 column eluted by the Gradient-II with various buffers (from bottom to top: 0.01% formic acid, 2 mM ammonium formate at pH 3.5, 2 mM ammonium acetate at pH 4.5, 2 mM ammonium acetate at pH 5.5, 2 mM ammonium bicarbonate at pH 6.5 and 2 mM ammonium bicarbonate at pH 7.5) and acetonitrile as the aqueous phase and the organic phase respectively.

    Journal: Analytical and bioanalytical chemistry

    Article Title: Factors affecting separation and detection of bile acids by liquid chromatography coupled with mass spectrometry at negative mode

    doi: 10.1007/s00216-017-0489-1

    Figure Lengend Snippet: Ion chromatograms of unconjugated BAs on an ACQUITY BEH C18 column eluted by the Gradient-II with various buffers (from bottom to top: 0.01% formic acid, 2 mM ammonium formate at pH 3.5, 2 mM ammonium acetate at pH 4.5, 2 mM ammonium acetate at pH 5.5, 2 mM ammonium bicarbonate at pH 6.5 and 2 mM ammonium bicarbonate at pH 7.5) and acetonitrile as the aqueous phase and the organic phase respectively.

    Article Snippet: The Gradient-I with a run time of 20 min utilized ACQUITY BEH C18 column (1.7 μm, 100 mm × 2.1 mm) (Waters, Milford, MA, USA) maintained at 45 °C.

    Techniques:

    Retention time of unconjugated BAs eluted by the key intermediate gradient programs during optimization of the Gradient-II on an ACQUITY BEH C18 column. Mobile phase A: 0.01% formic acid (v/v) in water; Mobile phase B: acetonitrile; Blue square: monohydroxyl-BAs (isoLCA/ alloLCA/LCA, blue square); Green diamond: dihydroxyl-BAs (muroCA/βUDCA/UDCA/HDCA/ CDCA/DCA/isoDCA); Red circle: trihydroxyl-BAs (βUCA/UCA/ωMCA/βCA/αMCA/βMCA/HCA/ ACA/ CA).

    Journal: Analytical and bioanalytical chemistry

    Article Title: Factors affecting separation and detection of bile acids by liquid chromatography coupled with mass spectrometry at negative mode

    doi: 10.1007/s00216-017-0489-1

    Figure Lengend Snippet: Retention time of unconjugated BAs eluted by the key intermediate gradient programs during optimization of the Gradient-II on an ACQUITY BEH C18 column. Mobile phase A: 0.01% formic acid (v/v) in water; Mobile phase B: acetonitrile; Blue square: monohydroxyl-BAs (isoLCA/ alloLCA/LCA, blue square); Green diamond: dihydroxyl-BAs (muroCA/βUDCA/UDCA/HDCA/ CDCA/DCA/isoDCA); Red circle: trihydroxyl-BAs (βUCA/UCA/ωMCA/βCA/αMCA/βMCA/HCA/ ACA/ CA).

    Article Snippet: The Gradient-I with a run time of 20 min utilized ACQUITY BEH C18 column (1.7 μm, 100 mm × 2.1 mm) (Waters, Milford, MA, USA) maintained at 45 °C.

    Techniques: High Content Screening

    Retention variations with the pH value of mobile phases for the unconjugated BAs (A, C) and the conjugated BAs (B, D) on BEH C18 column (A, B) and HSS T3 column (C, D).

    Journal: Analytical and bioanalytical chemistry

    Article Title: Factors affecting separation and detection of bile acids by liquid chromatography coupled with mass spectrometry at negative mode

    doi: 10.1007/s00216-017-0489-1

    Figure Lengend Snippet: Retention variations with the pH value of mobile phases for the unconjugated BAs (A, C) and the conjugated BAs (B, D) on BEH C18 column (A, B) and HSS T3 column (C, D).

    Article Snippet: The Gradient-I with a run time of 20 min utilized ACQUITY BEH C18 column (1.7 μm, 100 mm × 2.1 mm) (Waters, Milford, MA, USA) maintained at 45 °C.

    Techniques:

    FKA concentrations in plasma and urine and its relationship with bladder weight of male UPII-SV40T transgenic mice A, Chromatographic separation of FKA was achieved using an ACQUITY UPLC (Waters, UK) with a reversed-phase ACQUITY UPLCTM BEP C18 column (1.7μm, 2.1× 50mm, Waters). FKA was monitored as a precursor ion with an m/z value of 315 and a fragment ion with a value at 181.14. A, a representative chromatograph of the plasma level of FKA after 0.6% dietary FKA feeding for 290 days in a male UPII-SV40T transgenic mouse. Flavokawain B (FKB) was used as an internal control. B, The mean FKA concentrations in plasma and urine in mice fed with vehicle control or 0.6% FKA for 290 days. Bar: mean ± SD. C, Correlation analysis between FKA plasma concentrations and bladder weights in male low copyUPII-SV40T transgenic mice. D, Correlation analysis between FKA plasma concentrations and bladder weights in female low copy UPII-SV40T transgenic mice.

    Journal: Cancer prevention research (Philadelphia, Pa.)

    Article Title: KAVA chalcone, Flavokawain A, inhibits urothelial tumorigenesis in the UPII-SV40T transgenic mouse model

    doi: 10.1158/1940-6207.CAPR-13-0219

    Figure Lengend Snippet: FKA concentrations in plasma and urine and its relationship with bladder weight of male UPII-SV40T transgenic mice A, Chromatographic separation of FKA was achieved using an ACQUITY UPLC (Waters, UK) with a reversed-phase ACQUITY UPLCTM BEP C18 column (1.7μm, 2.1× 50mm, Waters). FKA was monitored as a precursor ion with an m/z value of 315 and a fragment ion with a value at 181.14. A, a representative chromatograph of the plasma level of FKA after 0.6% dietary FKA feeding for 290 days in a male UPII-SV40T transgenic mouse. Flavokawain B (FKB) was used as an internal control. B, The mean FKA concentrations in plasma and urine in mice fed with vehicle control or 0.6% FKA for 290 days. Bar: mean ± SD. C, Correlation analysis between FKA plasma concentrations and bladder weights in male low copyUPII-SV40T transgenic mice. D, Correlation analysis between FKA plasma concentrations and bladder weights in female low copy UPII-SV40T transgenic mice.

    Article Snippet: Separation of compounds was carried out at 50°C using a Waters Acquity UPLC ®BEH C18 1.7 μm (2.1×50 mm) column with a gradient elution: (A) Water: Acetonitrile: acetic acid (97.8:2:0.2 v/v/v), and (B) Acetonitrile: acetic acid 99.8:0.2 (v/v) as the mobile phase.

    Techniques: Transgenic Assay, Mouse Assay

    UPLC-MS/MS results in the negative mode using a BEH amide column and ACN/H 2 O gradient for analysis of barley β-glucan (BBG) oxidation products (harsh conditions) after enzymatic digestion and SPE (strategy II). (A) Analysis of the neutral products using basic 0.1% NH 3 eluent additive (oxo-product peaks with carbonyl group at the non-reducing end oxo GlcGlc ( n −1) in red) and (B) their respective MS/MS spectra. The main oxo-Glc 5 species with C=O at a different position is also included (bottom spectrum). (C) Analysis of acidic products using a buffered 60 mM ammonium formate eluent additive (pH ~ 8) with glucuronic acid bearing oligomers GlcAGlc ( n −1) in red. (D) Respective MS/MS spectra of acidic products. The MS/MS fragments are named according to the nomenclature of Domon and Costello ( 1988 ) (cleaved linkage: C i fragments; cross-ring cleavage: A i fragments; i = 1, 2, … n ). * oxo-products with oxidized carbonyl group not at the non-reducing end; ** Disaccharide signal from catalase material; *** Buffer salt/solvent peak.

    Journal: Frontiers in Chemistry

    Article Title: Complementary Sample Preparation Strategies for Analysis of Cereal β-Glucan Oxidation Products by UPLC-MS/MS

    doi: 10.3389/fchem.2017.00090

    Figure Lengend Snippet: UPLC-MS/MS results in the negative mode using a BEH amide column and ACN/H 2 O gradient for analysis of barley β-glucan (BBG) oxidation products (harsh conditions) after enzymatic digestion and SPE (strategy II). (A) Analysis of the neutral products using basic 0.1% NH 3 eluent additive (oxo-product peaks with carbonyl group at the non-reducing end oxo GlcGlc ( n −1) in red) and (B) their respective MS/MS spectra. The main oxo-Glc 5 species with C=O at a different position is also included (bottom spectrum). (C) Analysis of acidic products using a buffered 60 mM ammonium formate eluent additive (pH ~ 8) with glucuronic acid bearing oligomers GlcAGlc ( n −1) in red. (D) Respective MS/MS spectra of acidic products. The MS/MS fragments are named according to the nomenclature of Domon and Costello ( 1988 ) (cleaved linkage: C i fragments; cross-ring cleavage: A i fragments; i = 1, 2, … n ). * oxo-products with oxidized carbonyl group not at the non-reducing end; ** Disaccharide signal from catalase material; *** Buffer salt/solvent peak.

    Article Snippet: UPLC-MS/MS analysis A Waters Acquity UPLC system equipped with an Acquity UPLC BEH Amide column (2.1 × 150 mm, 1.7 μm) was used unless otherwise noted, coupled to a Synapt G2 MS system composed of an electrospray ionization (ESI) source and a quadrupole time-of-flight (qToF) analyzer (Waters Corp., Milford, MA, USA) as described earlier (Boulos and Nyström, ).

    Techniques: Mass Spectrometry, Gas Chromatography

    (A) UPLC-MS base peak chromatogram in the negative ionization mode using a BEH amide column and ACN/H 2 O gradient (0.1% NH 3 ) for analysis of the released oligosaccharides from barley β-glucan (BBG) oxidation (harsh conditions) extracted by SPE and concentrated (sample preparation strategy I). Note the 6x zoom for region 6–12 min. Peaks are labeled with their respective base peak m/z and with n = number of monosaccharide units in blue for gluco-oligosaccharides Glc n and in red for oxo-Glc n species (with letter qualifier if same n occurs more than once, e.g., 3a, 3b…). (B) MS spectrum of the acidic products obtained from the indicated chromatogram region in (A) . Signals are labeled with m/z , with the glucose-carbon location of the carboxylic acid (C1, C6, or C1+C6), and with type of reducing end if cross-ring cleaved to arabinose (Ara). Glc, glucose; oxo-Glc n , oligomer Glc n oxidized anywhere along the chain (new C=O); Glc 3 Ara/Ery, glucotriose linked to arabinose/erythrose end group; Glc1A, gluconic acid group (C1 oxidized); GlcA, glucuronic acid group (C6 oxidized).

    Journal: Frontiers in Chemistry

    Article Title: Complementary Sample Preparation Strategies for Analysis of Cereal β-Glucan Oxidation Products by UPLC-MS/MS

    doi: 10.3389/fchem.2017.00090

    Figure Lengend Snippet: (A) UPLC-MS base peak chromatogram in the negative ionization mode using a BEH amide column and ACN/H 2 O gradient (0.1% NH 3 ) for analysis of the released oligosaccharides from barley β-glucan (BBG) oxidation (harsh conditions) extracted by SPE and concentrated (sample preparation strategy I). Note the 6x zoom for region 6–12 min. Peaks are labeled with their respective base peak m/z and with n = number of monosaccharide units in blue for gluco-oligosaccharides Glc n and in red for oxo-Glc n species (with letter qualifier if same n occurs more than once, e.g., 3a, 3b…). (B) MS spectrum of the acidic products obtained from the indicated chromatogram region in (A) . Signals are labeled with m/z , with the glucose-carbon location of the carboxylic acid (C1, C6, or C1+C6), and with type of reducing end if cross-ring cleaved to arabinose (Ara). Glc, glucose; oxo-Glc n , oligomer Glc n oxidized anywhere along the chain (new C=O); Glc 3 Ara/Ery, glucotriose linked to arabinose/erythrose end group; Glc1A, gluconic acid group (C1 oxidized); GlcA, glucuronic acid group (C6 oxidized).

    Article Snippet: UPLC-MS/MS analysis A Waters Acquity UPLC system equipped with an Acquity UPLC BEH Amide column (2.1 × 150 mm, 1.7 μm) was used unless otherwise noted, coupled to a Synapt G2 MS system composed of an electrospray ionization (ESI) source and a quadrupole time-of-flight (qToF) analyzer (Waters Corp., Milford, MA, USA) as described earlier (Boulos and Nyström, ).

    Techniques: Mass Spectrometry, Sample Prep, Labeling, Gas Chromatography, Acetylene Reduction Assay

    Formation of reaction products of the 6-plex enzyme assay. (A) Representative chromatograms of the reaction products of six LSD enzymes. A BEH C18 column (2 × 100 mm) was used. The arrows indicate the product of the enzyme reaction. P, product. (B) Time-dependent accumulation of enzyme reaction products of the LSD assay. The concentrations of the enzyme reaction products were determined at 0, 4 or 20 h of incubation at 37 °C. Details of the method are provided in the Experimental procedure section.

    Journal: Molecular Genetics and Metabolism Reports

    Article Title: Levels of enzyme activities in six lysosomal storage diseases in Japanese neonates determined by liquid chromatography-tandem mass spectrometry

    doi: 10.1016/j.ymgmr.2016.08.007

    Figure Lengend Snippet: Formation of reaction products of the 6-plex enzyme assay. (A) Representative chromatograms of the reaction products of six LSD enzymes. A BEH C18 column (2 × 100 mm) was used. The arrows indicate the product of the enzyme reaction. P, product. (B) Time-dependent accumulation of enzyme reaction products of the LSD assay. The concentrations of the enzyme reaction products were determined at 0, 4 or 20 h of incubation at 37 °C. Details of the method are provided in the Experimental procedure section.

    Article Snippet: The following analytical columns were tested: an ACQUITY BEH C18 (1.7 μm, 100 × 2.1 mm) from Waters, a Chromolith RP-2 (3 μm, 100 × 2.1 mm) from Merck-Millipore (Tokyo, Japan), and a MonoTower C18 (3 μm, 100 × 2.1 mm) from GL Sciences (Tokyo, Japan).

    Techniques: Enzymatic Assay, Incubation