acly inhibitor sb204990 Search Results


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    • acly inhibitor sb 204990  (Millipore)
    86
    Millipore acly inhibitor sb 204990
    ( A ) HCT116 cells were plated at 60% confluence in 96-well plate in complete medium (CM) containing 10% FBS. After 24 hr, cells were shifted to medium with or without serum. Next, cells were transferred into media containing concentrations of 10 μM, 20 μM, 40 μM, and 80 μM ACLYi <t>(SB-204990)</t> for 24 hr. XTT assay was performed for cell viability. ( B ) HCT116 cells were plated as in A . After 24 hr, cells were shifted to medium with or without serum. Next, cells were transferred into media containing SB-204990 (40 μM) for 12 hr, 24 hr, 48 hr, and 96 hr time points. XTT assay was performed for cell viability. ( C ) MDA-MB-231 cells were plated and treated as in A . XTT assay was performed for cell viability. ( D ) BJ-hTERT cells were plated and treated as in B . XTT assay was performed for cell viability. All experiments were repeated two or more times. Error bars represent ± SEM. Unpaired Student’s t-test was performed for statistical analysis.
    Acly Inhibitor Sb 204990, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acly inhibitor sb 204990/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acly inhibitor sb 204990 - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier
    acly inhibitor sb 204990  (Tocris)
    86
    Tocris acly inhibitor sb 204990
    ( A ) HCT116 cells were plated at 60% confluence in 96-well plate in complete medium (CM) containing 10% FBS. After 24 hr, cells were shifted to medium with or without serum. Next, cells were transferred into media containing concentrations of 10 μM, 20 μM, 40 μM, and 80 μM ACLYi <t>(SB-204990)</t> for 24 hr. XTT assay was performed for cell viability. ( B ) HCT116 cells were plated as in A . After 24 hr, cells were shifted to medium with or without serum. Next, cells were transferred into media containing SB-204990 (40 μM) for 12 hr, 24 hr, 48 hr, and 96 hr time points. XTT assay was performed for cell viability. ( C ) MDA-MB-231 cells were plated and treated as in A . XTT assay was performed for cell viability. ( D ) BJ-hTERT cells were plated and treated as in B . XTT assay was performed for cell viability. All experiments were repeated two or more times. Error bars represent ± SEM. Unpaired Student’s t-test was performed for statistical analysis.
    Acly Inhibitor Sb 204990, supplied by Tocris, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acly inhibitor sb 204990/product/Tocris
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acly inhibitor sb 204990 - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) HCT116 cells were plated at 60% confluence in 96-well plate in complete medium (CM) containing 10% FBS. After 24 hr, cells were shifted to medium with or without serum. Next, cells were transferred into media containing concentrations of 10 μM, 20 μM, 40 μM, and 80 μM ACLYi (SB-204990) for 24 hr. XTT assay was performed for cell viability. ( B ) HCT116 cells were plated as in A . After 24 hr, cells were shifted to medium with or without serum. Next, cells were transferred into media containing SB-204990 (40 μM) for 12 hr, 24 hr, 48 hr, and 96 hr time points. XTT assay was performed for cell viability. ( C ) MDA-MB-231 cells were plated and treated as in A . XTT assay was performed for cell viability. ( D ) BJ-hTERT cells were plated and treated as in B . XTT assay was performed for cell viability. All experiments were repeated two or more times. Error bars represent ± SEM. Unpaired Student’s t-test was performed for statistical analysis.

    Journal: PLoS ONE

    Article Title: Inhibiting glutamine utilization creates a synthetic lethality for suppression of ATP citrate lyase in KRas-driven cancer cells

    doi: 10.1371/journal.pone.0276579

    Figure Lengend Snippet: ( A ) HCT116 cells were plated at 60% confluence in 96-well plate in complete medium (CM) containing 10% FBS. After 24 hr, cells were shifted to medium with or without serum. Next, cells were transferred into media containing concentrations of 10 μM, 20 μM, 40 μM, and 80 μM ACLYi (SB-204990) for 24 hr. XTT assay was performed for cell viability. ( B ) HCT116 cells were plated as in A . After 24 hr, cells were shifted to medium with or without serum. Next, cells were transferred into media containing SB-204990 (40 μM) for 12 hr, 24 hr, 48 hr, and 96 hr time points. XTT assay was performed for cell viability. ( C ) MDA-MB-231 cells were plated and treated as in A . XTT assay was performed for cell viability. ( D ) BJ-hTERT cells were plated and treated as in B . XTT assay was performed for cell viability. All experiments were repeated two or more times. Error bars represent ± SEM. Unpaired Student’s t-test was performed for statistical analysis.

    Article Snippet: The ACLY inhibitor SB-204990 (Sigma SML2829), OA (Sigma O3008), fatty acid-free bovine serum albumin (BSA) (Sigma A9205), dimethyl α-ketoglutarate (DMKG) (Sigma 349631) were purchased from Sigma.

    Techniques: XTT Assay

    ( A ) MDA-MB-231 cells were plated at 50% confluence in 96-well plates in complete medium (CM) containing 10% FBS. After 24 hr, cells were shifted to medium containing or lacking glutamine for 48 h. For the last 24 hr, cells were transferred into media containing SB-204990 (40 μM) with or without DMKG. Plates were read after 24 hr in plate reader and analyzed for cytotoxicity via XTT assay. Lysates were collected and analyzed for protein expression via western blotting. (B ) MDA-MB-231 cells were plated as in A . After 24 hr, cells were shifted to medium containing or lacking AOA for 48 hr. For the last 24 hr, cells were transferred into media containing SB-204990 (40 μM) with or without DMKG (4 mM). Plates were read after 24 hr in plate reader and analyzed for cytotoxicity via XTT assay. Lysates were collected and analyzed for protein expression via western blotting. All experiments were repeated two or more times. Error bars represent ± SEM. Unpaired Student’s t-test was performed for statistical analysis. The Western blots shown are representative of experiments repeated at least two times.

    Journal: PLoS ONE

    Article Title: Inhibiting glutamine utilization creates a synthetic lethality for suppression of ATP citrate lyase in KRas-driven cancer cells

    doi: 10.1371/journal.pone.0276579

    Figure Lengend Snippet: ( A ) MDA-MB-231 cells were plated at 50% confluence in 96-well plates in complete medium (CM) containing 10% FBS. After 24 hr, cells were shifted to medium containing or lacking glutamine for 48 h. For the last 24 hr, cells were transferred into media containing SB-204990 (40 μM) with or without DMKG. Plates were read after 24 hr in plate reader and analyzed for cytotoxicity via XTT assay. Lysates were collected and analyzed for protein expression via western blotting. (B ) MDA-MB-231 cells were plated as in A . After 24 hr, cells were shifted to medium containing or lacking AOA for 48 hr. For the last 24 hr, cells were transferred into media containing SB-204990 (40 μM) with or without DMKG (4 mM). Plates were read after 24 hr in plate reader and analyzed for cytotoxicity via XTT assay. Lysates were collected and analyzed for protein expression via western blotting. All experiments were repeated two or more times. Error bars represent ± SEM. Unpaired Student’s t-test was performed for statistical analysis. The Western blots shown are representative of experiments repeated at least two times.

    Article Snippet: The ACLY inhibitor SB-204990 (Sigma SML2829), OA (Sigma O3008), fatty acid-free bovine serum albumin (BSA) (Sigma A9205), dimethyl α-ketoglutarate (DMKG) (Sigma 349631) were purchased from Sigma.

    Techniques: XTT Assay, Expressing, Western Blot

    Mitochondrial use of citrate is mainly catabolic via sequential oxidative decarboxylation reactions while cytosolic utilization of citrate yields anabolic precursors. Metabolic reprogramming in KRas-driven cancer cells changes the fate of citrate from mitochondrial catabolism to the anabolic ACLY pathway. Blocking ACLY in combination with blocking GOT disturbs the reprogramming in KRas-driven cancer cells creating a synthetic lethal situation where cancer cells harboring KRas mutations undergo apoptotic cell death. Additional abbreviations: ACLYi, ACLY inhibitor (SB-204990); Cyto, cytoplasm; DHAP, dihydroxyacetone phosphate; ER, endoplasmic reticulum; GA3P, glyceraldehyde 3-phosphate; G3P, glycerol 3-phosphate; Mito, mitochondrion.

    Journal: PLoS ONE

    Article Title: Inhibiting glutamine utilization creates a synthetic lethality for suppression of ATP citrate lyase in KRas-driven cancer cells

    doi: 10.1371/journal.pone.0276579

    Figure Lengend Snippet: Mitochondrial use of citrate is mainly catabolic via sequential oxidative decarboxylation reactions while cytosolic utilization of citrate yields anabolic precursors. Metabolic reprogramming in KRas-driven cancer cells changes the fate of citrate from mitochondrial catabolism to the anabolic ACLY pathway. Blocking ACLY in combination with blocking GOT disturbs the reprogramming in KRas-driven cancer cells creating a synthetic lethal situation where cancer cells harboring KRas mutations undergo apoptotic cell death. Additional abbreviations: ACLYi, ACLY inhibitor (SB-204990); Cyto, cytoplasm; DHAP, dihydroxyacetone phosphate; ER, endoplasmic reticulum; GA3P, glyceraldehyde 3-phosphate; G3P, glycerol 3-phosphate; Mito, mitochondrion.

    Article Snippet: The ACLY inhibitor SB-204990 (Sigma SML2829), OA (Sigma O3008), fatty acid-free bovine serum albumin (BSA) (Sigma A9205), dimethyl α-ketoglutarate (DMKG) (Sigma 349631) were purchased from Sigma.

    Techniques: Blocking Assay