Journal:

Article Title: Defining Aggressive Prostate Cancer Using a 12-Gene Model 1

doi:

Figure Lengend Snippet: Protein expression of 41 genes selected for differential expression in prostate cancer progression. (A) A “heat map” showing the relative protein expression of 41 genes selected as highly likely to demonstrate differential expression in prostate tissue samples along the spectrum of cancer progression. Protein expression was determined using antibodies directed against gene products and measured by immunohistochemistry and using a semiautomated image analysis system (ACIS II; Chromavision Medical Systems, Inc.), which measures staining intensity along a continuous scale from 0 to 255. Low and high expressions are depicted using light green and bright red, respectively. Hierarchical clustering of the samples demonstrates a good—but not perfect—ability of this 41-gene panel to distinguish between classes. (B) Although some subtypes of metastatic prostate cancer (small cell cancer) (upper figure; original magnification, x 200) had discrete profiles, the clustering did not accurately distinguish between all of the hormone-naïve and hormone-refractory prostate tumor samples. Interestingly, a high-grade LPCa (Gleason pattern 4 prostate cancer) (lower figure; original magnification, x 200) was found to cluster more closely to the metastatic samples using this 41-gene profile.

Article Snippet: Quantitative Biomarker Analysis Protein expression was evaluated by immunohistochemistry using a semiautomated quantitative image analysis system ACIS II (Chromavision Medical Systems, Inc., San Juan Capistrano, CA).

Techniques: Expressing, Quantitative Proteomics, Immunohistochemistry, Staining