Journal: Frontiers in Microbiology

Article Title: Differential Impacts of Willow and Mineral Fertilizer on Bacterial Communities and Biodegradation in Diesel Fuel Oil-Contaminated Soil

doi: 10.3389/fmicb.2016.00837

Figure Lengend Snippet: Taxa deriving carbon from naphthalene, either directly or via cross-feeding, as detected at different time points.

Article Snippet: Hydrogenophaga , Hydrogenophaga taeniospiralis ATCC 49743 , AF078768 , 0.994 , 1.1 , 1.6 , 5.1 , 0.6 , 0.9 , 0.0 , 1.4 , 10.1.

Techniques: Sequencing

Journal: Applied and Environmental Microbiology

Article Title: A Novel Aerobic Degradation Pathway for Thiobencarb Is Initiated by the TmoAB Two-Component Flavin Mononucleotide-Dependent Monooxygenase System in Acidovorax sp. Strain T1

doi: 10.1128/AEM.01490-17

Figure Lengend Snippet: GC-MS spectra of the silanized derivatives of the intermediates during thiobencarb degradation by Acidovorax sp. T1. (A) GC spectrum of the silanized derivatives of the intermediates. (B) Mass spectrum of the product at GC retention time 7.56 min. (C) Mass spectrum of the product at GC retention time 9.57 min.

Article Snippet: Acidovorax sp. T1 (= CCTCC M2016205) , Degrades thiobencarb , This study.

Techniques: Gas Chromatography-Mass Spectrometry

Journal: Applied and Environmental Microbiology

Article Title: A Novel Aerobic Degradation Pathway for Thiobencarb Is Initiated by the TmoAB Two-Component Flavin Mononucleotide-Dependent Monooxygenase System in Acidovorax sp. Strain T1

doi: 10.1128/AEM.01490-17

Figure Lengend Snippet: Degradation mechanism of thiobencarb in Acidovorax sp. T1. (A) Degradation pathway for thiobencarb. (B) Genome of Acidovorax sp. T1, containing one circular chromosome and three circular plasmids (P1, P2, and P3). The scale for each replicon is marked on the black scale circle. The positions of gene clusters for thiobencarb degradation and 4CBA degradation are marked by the outermost red arcs. The first and second circles from the center indicate the GC skew (G−C/G+C) and G+C content: for GC skew, the parts greater than and less than zero are colored pink and light blue, respectively; for G+C content, the parts greater than and less than zero are colored red and blue, respectively. The third circle represents rRNA (red) and tRNA (gray). The fourth and fifth circles show CDSs (coding sequences) on the negative and positive DNA strands, respectively. (C) Organization of the tmoA, tmoB, and tmoC genes in plasmid P3. (D) Organization of the lig, pob, and fcb operons in the chromosome.

Article Snippet: Acidovorax sp. T1 (= CCTCC M2016205) , Degrades thiobencarb , This study.

Techniques: Plasmid Preparation

Journal: Applied and Environmental Microbiology

Article Title: A Novel Aerobic Degradation Pathway for Thiobencarb Is Initiated by the TmoAB Two-Component Flavin Mononucleotide-Dependent Monooxygenase System in Acidovorax sp. Strain T1

doi: 10.1128/AEM.01490-17

Figure Lengend Snippet: Transcriptional levels of tmoA, tmoB, tmoC, fcbB, pobA, and ligB in Acidovorax sp. T1 with or without thiobencarb induction. Relative expression levels were calculated using the 2−ΔΔCT threshold cycle (CT) method with the 16S rRNA gene used as the reference gene. The data were derived from three independent measurements, and the error bars indicate standard deviations.

Article Snippet: Acidovorax sp. T1 (= CCTCC M2016205) , Degrades thiobencarb , This study.

Techniques: Expressing, Derivative Assay

Journal: Applied and Environmental Microbiology

Article Title: A Novel Aerobic Degradation Pathway for Thiobencarb Is Initiated by the TmoAB Two-Component Flavin Mononucleotide-Dependent Monooxygenase System in Acidovorax sp. Strain T1

doi: 10.1128/AEM.01490-17

Figure Lengend Snippet: Strains and vectors used in this study

Article Snippet: Acidovorax sp. T1 (= CCTCC M2016205) , Degrades thiobencarb , This study.

Techniques: Plasmid Preparation, Mutagenesis, Clone Assay, Expressing