Journal: Cell stem cell

Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy

doi: 10.1016/j.stem.2023.08.013

Figure Lengend Snippet: (A,B) Simplified schematics of neuronal subtypes derived from the MGE progenitor domain (FOXG1+ DLX1/2+ NKX2-1+), including GABAergic pallial interneurons (pINs) that migrate to the cortex and hippocampus (HC), as well as multiple lineages that remain in the subpallium. The latter include striatal GABAergic and cholinergic INs, cholinergic projection neurons (PN) of the basal telencephalon, and GABAergic PNs of the globus pallidus. Listed genes expressed in the immature neurons play critical roles in the specification of the indicated neuronal subtypes. (C,D) Representative ICC images of unsorted and sorted lot 1 after cryopreservation and thaw show expression of MGE pIN markers (LHX6, MAFB, MAF, ERBB4), and cholinergic neurons (ISL1). (E) Protein quantification across independent lots (n=10 to 18). (F,G) Quantification of GABA (F) and Acetylcholine (G) neurotransmitters in the culture supernatant from independent pIN lots (n=8), undifferentiated hESCs (n=1) and spinal motor neurons (n=1). (H) Cell viability post-thaw from independent pIN lots (n=16 unsorted/sorted pairs). In E-H, each dot is an average of technical replicates (2-3) from independently manufactured lots. All data are expressed as a mean ± SEM.

Article Snippet: The samples were submitted for liquid chromatography and mass spectrometry (LC/MS-MS) analysis of GABA and acetylcholine (ACh) neurotransmitter content (Charles Rivers Laboratories).

Techniques: Derivative Assay, Expressing

Journal: Frontiers in Pharmacology

Article Title: α-Bisabolol alleviates doxorubicin-induced cognitive dysfunction in rats via enhancing the hippocampal BDNF/TrKB signaling and inhibiting neuroinflammation

doi: 10.3389/fphar.2025.1549009

Figure Lengend Snippet: Effect of α-bisabolol on the protein expression of (A) BDNF, (B) TrKB, (C) Ach, (D) AchE, (E) 5-HT, (F) DA, (G) glutamate, (H) GABA in rat’s hippocampus in Dox-induced chemobrain. The findings are expressed as mean ± SEM (n = 6). Bars carrying various letters are substantially different (p < 0.05). BISA50, BISA100: α-bisabolol was introduced orally at 50 and 100 mg/kg/day, DOX: doxorubicin was injected i. p. Weekly for 4 weeks. BDNF: brain derived neurotropic factor, TrKB: tropomyosin receptor kinase B, ACh: acetylcholine, 5-HT: serotonin, DA: dopamine AChE: acetylcholinesterase, GABA: Gamma-aminobutyric acid.

Article Snippet: ELISA was utilized to estimate the hippocampal levels of interleukin-1β (IL-1β), IL-6 (Bioassay Technology Laboratory Co. Shanghai, China), total Nuclear factor kappa B (NF-kB, the total protein level from both nuclear and cytoplasmic fractions), acetylcholinesterase activity (AChE), concentrations of Gamma-aminobutyric acid (GABA) (Fine Test Co., Boulder, CO, United States), acetylcholine (Ach, Elabscience Co. Houston, TX, United States), serotonin (5-HT, AFG Scientific, Wood Dale, IL, United States), dopamine (DA, Cusabio Co., Houston, TX, United States), glutamate (Novus Biologicals Co., Centennial, Co., United States), BDNF (Biosensis Pty Ltd., Thebarton, SA, Australia), and TrkB (Antibodies co. Cambridge, United Kingdom).

Techniques: Expressing, Injection, Derivative Assay