acetonitrile acn Millipore Search Results


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  • 95
    Millipore acetonitrile
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Acetonitrile, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 4689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore acetonitrile acn
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Acetonitrile Acn, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore acetonitrile acn succinic d0 anhydride sigma
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
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    94
    Millipore acetonitrile acn trifluoroacetic acid
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Acetonitrile Acn Trifluoroacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore acn thf
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Acn Thf, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore liquid chromatography mass spectrometry lc ms grade acetonitrile acn
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Liquid Chromatography Mass Spectrometry Lc Ms Grade Acetonitrile Acn, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore acetonitrile water
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
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    95
    Millipore high performance liquid chromatography hplc grade acetonitrile
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    High Performance Liquid Chromatography Hplc Grade Acetonitrile, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Millipore reference material irmm 316
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Reference Material Irmm 316, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore reagents hplc grade acetonitrile
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Reagents Hplc Grade Acetonitrile, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore trifluoroacetic acid acn tfa
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Trifluoroacetic Acid Acn Tfa, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore hplc
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Hplc, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 952 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore hplc grade solvents
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Hplc Grade Solvents, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore hydroxytoluene
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Hydroxytoluene, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 726 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore buffer a
    Kinetic changes during <t>JBH</t> treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) <t>ACN</t> fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.
    Buffer A, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 10858 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Kinetic changes during JBH treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) ACN fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.

    Journal: Glycobiology

    Article Title: Hyaluronan synthase assembles hyaluronan on a [GlcNAc(β1,4)]n-GlcNAc(α1→)UDP primer and hyaluronan retains this residual chitin oligomer as a cap at the nonreducing end

    doi: 10.1093/glycob/cwx012

    Figure Lengend Snippet: Kinetic changes during JBH treatment show that G 3 H 2 and G 3 H 3 species from SeHAS HA are sequentially converted to smaller members of the H 2 and H 3 series. Aliquots (5 µL) of Wash 3 were incubated with 22 µL inactive (time-zero) or active JBH at 30°C for up to 26 h ( n = 4 except n = 3 at 26 h); values are the mean intensity ± SEM as indicated. Members of the G 0–3 H 3 (A) and G 0–3 H 2 (B) series were analyzed to monitor decreases or increases in each member as a function of increasing JBH digestion time, (G 3 H 2,3 , ●; G 2 H 2,3 , ▼; G 1 H 2,3 , ▲; G 0 H 2,3 , ■). Lines ( r 2 ≥ 0.97) for H 2,3 (solid) and G 3 H 2,3 (long dash) were calculated by linear regression or a 3-parameter hyperbolic decay function, respectively, using Sigma Plot v10. Lines for G 2 H 2,3 (short dash) and G 1 H 2,3 (dotted) are comprised of linear segments. (C) ACN fraction samples (10 μL) containing G 4 H 2 and much less or no smaller members of this G n H 2 series were incubated for up to 12 h, as indicated, with 22 µL of inactive (time-zero) or active JBH. Sequential and time-dependent changes in the levels of G 3 H 2 , G 2 H 2 , G 1 H 2 and H 2 are evident.

    Article Snippet: Jack bean β-N-acetylhexosaminidase (JBH, #A2264), Streptomyces hyalurolyticus hyaluronidase (HA lyase, #H1136), OTH (#H-6254), and ACN (HPLC grade) were from Sigma-Aldrich (St. Louis, MO).

    Techniques: Incubation

    JBH digestion of G n H n species from SeHAS HA releases nondehydro NR-end H 2 and H 3 oligos. A–F. Selected m/z ranges are shown for Wash 3 (0.5 μL) samples treated overnight at 30°C with inactive (A, C, E) or active (B, D, F) JBH and analyzed by MALDI-TOF MS in negative mode. G n H n species that decrease or increase, with active JBH treatment, are indicated in italic or boldface font, respectively. New m/z signals are evident corresponding to H 2 (B compared to A), H 3 (D compared to C) and H 4 (F compared to E). (G–J) ACN samples (1, 2 or 3 µL), as indicated, were incubated overnight at 30°C with active or inactive JBH, and the presence of m / z signals, proportionally increasing with sample volume, was monitored for H 2 (panel G) or H 3 (panel H). The m / z signal decreases for the larger G 4 H 3 (I) and G 4 H 2 (J) ions were assessed in the 3 µL samples, treated with active (top) or inactive (bottom) JBH.

    Journal: Glycobiology

    Article Title: Hyaluronan synthase assembles hyaluronan on a [GlcNAc(β1,4)]n-GlcNAc(α1→)UDP primer and hyaluronan retains this residual chitin oligomer as a cap at the nonreducing end

    doi: 10.1093/glycob/cwx012

    Figure Lengend Snippet: JBH digestion of G n H n species from SeHAS HA releases nondehydro NR-end H 2 and H 3 oligos. A–F. Selected m/z ranges are shown for Wash 3 (0.5 μL) samples treated overnight at 30°C with inactive (A, C, E) or active (B, D, F) JBH and analyzed by MALDI-TOF MS in negative mode. G n H n species that decrease or increase, with active JBH treatment, are indicated in italic or boldface font, respectively. New m/z signals are evident corresponding to H 2 (B compared to A), H 3 (D compared to C) and H 4 (F compared to E). (G–J) ACN samples (1, 2 or 3 µL), as indicated, were incubated overnight at 30°C with active or inactive JBH, and the presence of m / z signals, proportionally increasing with sample volume, was monitored for H 2 (panel G) or H 3 (panel H). The m / z signal decreases for the larger G 4 H 3 (I) and G 4 H 2 (J) ions were assessed in the 3 µL samples, treated with active (top) or inactive (bottom) JBH.

    Article Snippet: Jack bean β-N-acetylhexosaminidase (JBH, #A2264), Streptomyces hyalurolyticus hyaluronidase (HA lyase, #H1136), OTH (#H-6254), and ACN (HPLC grade) were from Sigma-Aldrich (St. Louis, MO).

    Techniques: Mass Spectrometry, Incubation