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Fig. 4. NPC patient-derived neurons show reduced neuronal network density. (A) Protocol for differentiation of iPSCs-derived NSCs into neurons. (B) <t>qPCR</t> analysis of neuronal markers in neurons. Run data were analyzed by the ΔΔCt method; cDNA from qPCR Human Reference Total RNA was used as a calibrator and β-ACTIN expression was used for normalization. Data are expressed as fold increases compared with levels in 201B7. *P < 0.05, **P < 0.01, and ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM). (C) Representative images of immunostaining for neuronal markers. Membrane-permeabilized neurons were stained with anti-TUJ1 antibody (green), anti-MAP2 antibody (white), and DAPI (blue). Scale bar = 100 μm. (D) Representative analysis images of TUJ1-positive neurites and DAPI-positive nuclei (scale bar = 200 μm). Neuronal network density was determined as TUJ1-positive %area divided by the number of DAPI-positive cell nuclei (the two latter variables were quantified using FIJI). **P < 0.01, ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM).
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Fig. 4. NPC patient-derived neurons show reduced neuronal network density. (A) Protocol for differentiation of iPSCs-derived NSCs into neurons. (B) <t>qPCR</t> analysis of neuronal markers in neurons. Run data were analyzed by the ΔΔCt method; cDNA from qPCR Human Reference Total RNA was used as a calibrator and β-ACTIN expression was used for normalization. Data are expressed as fold increases compared with levels in 201B7. *P < 0.05, **P < 0.01, and ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM). (C) Representative images of immunostaining for neuronal markers. Membrane-permeabilized neurons were stained with anti-TUJ1 antibody (green), anti-MAP2 antibody (white), and DAPI (blue). Scale bar = 100 μm. (D) Representative analysis images of TUJ1-positive neurites and DAPI-positive nuclei (scale bar = 200 μm). Neuronal network density was determined as TUJ1-positive %area divided by the number of DAPI-positive cell nuclei (the two latter variables were quantified using FIJI). **P < 0.01, ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM).
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Fig. 4. NPC patient-derived neurons show reduced neuronal network density. (A) Protocol for differentiation of iPSCs-derived NSCs into neurons. (B) <t>qPCR</t> analysis of neuronal markers in neurons. Run data were analyzed by the ΔΔCt method; cDNA from qPCR Human Reference Total RNA was used as a calibrator and β-ACTIN expression was used for normalization. Data are expressed as fold increases compared with levels in 201B7. *P < 0.05, **P < 0.01, and ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM). (C) Representative images of immunostaining for neuronal markers. Membrane-permeabilized neurons were stained with anti-TUJ1 antibody (green), anti-MAP2 antibody (white), and DAPI (blue). Scale bar = 100 μm. (D) Representative analysis images of TUJ1-positive neurites and DAPI-positive nuclei (scale bar = 200 μm). Neuronal network density was determined as TUJ1-positive %area divided by the number of DAPI-positive cell nuclei (the two latter variables were quantified using FIJI). **P < 0.01, ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM).
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anti human angiotensin  (Proteintech)
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Fig. 4. NPC patient-derived neurons show reduced neuronal network density. (A) Protocol for differentiation of iPSCs-derived NSCs into neurons. (B) <t>qPCR</t> analysis of neuronal markers in neurons. Run data were analyzed by the ΔΔCt method; cDNA from qPCR Human Reference Total RNA was used as a calibrator and β-ACTIN expression was used for normalization. Data are expressed as fold increases compared with levels in 201B7. *P < 0.05, **P < 0.01, and ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM). (C) Representative images of immunostaining for neuronal markers. Membrane-permeabilized neurons were stained with anti-TUJ1 antibody (green), anti-MAP2 antibody (white), and DAPI (blue). Scale bar = 100 μm. (D) Representative analysis images of TUJ1-positive neurites and DAPI-positive nuclei (scale bar = 200 μm). Neuronal network density was determined as TUJ1-positive %area divided by the number of DAPI-positive cell nuclei (the two latter variables were quantified using FIJI). **P < 0.01, ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM).
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Fig. 4. NPC patient-derived neurons show reduced neuronal network density. (A) Protocol for differentiation of iPSCs-derived NSCs into neurons. (B) <t>qPCR</t> analysis of neuronal markers in neurons. Run data were analyzed by the ΔΔCt method; cDNA from qPCR Human Reference Total RNA was used as a calibrator and β-ACTIN expression was used for normalization. Data are expressed as fold increases compared with levels in 201B7. *P < 0.05, **P < 0.01, and ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM). (C) Representative images of immunostaining for neuronal markers. Membrane-permeabilized neurons were stained with anti-TUJ1 antibody (green), anti-MAP2 antibody (white), and DAPI (blue). Scale bar = 100 μm. (D) Representative analysis images of TUJ1-positive neurites and DAPI-positive nuclei (scale bar = 200 μm). Neuronal network density was determined as TUJ1-positive %area divided by the number of DAPI-positive cell nuclei (the two latter variables were quantified using FIJI). **P < 0.01, ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM).
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anterior cingulate epilepsy ace  (MedChemExpress)
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Fig. 4. NPC patient-derived neurons show reduced neuronal network density. (A) Protocol for differentiation of iPSCs-derived NSCs into neurons. (B) <t>qPCR</t> analysis of neuronal markers in neurons. Run data were analyzed by the ΔΔCt method; cDNA from qPCR Human Reference Total RNA was used as a calibrator and β-ACTIN expression was used for normalization. Data are expressed as fold increases compared with levels in 201B7. *P < 0.05, **P < 0.01, and ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM). (C) Representative images of immunostaining for neuronal markers. Membrane-permeabilized neurons were stained with anti-TUJ1 antibody (green), anti-MAP2 antibody (white), and DAPI (blue). Scale bar = 100 μm. (D) Representative analysis images of TUJ1-positive neurites and DAPI-positive nuclei (scale bar = 200 μm). Neuronal network density was determined as TUJ1-positive %area divided by the number of DAPI-positive cell nuclei (the two latter variables were quantified using FIJI). **P < 0.01, ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM).
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gene exp ace hs00174179 m1  (Thermo Fisher)
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QRT-PCR Validation of Gene Array Results for Baboon Renal Cortex
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66 699 1 ig  (Proteintech)
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QRT-PCR Validation of Gene Array Results for Baboon Renal Cortex
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gene exp ace rn00561094 m1  (Thermo Fisher)
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QRT-PCR Validation of Gene Array Results for Baboon Renal Cortex
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gene exp ace mm00802048 m1  (Thermo Fisher)
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QRT-PCR Validation of Gene Array Results for Baboon Renal Cortex
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anti ace2  (ProSci Incorporated)
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QRT-PCR Validation of Gene Array Results for Baboon Renal Cortex
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Image Search Results


Fig. 4. NPC patient-derived neurons show reduced neuronal network density. (A) Protocol for differentiation of iPSCs-derived NSCs into neurons. (B) qPCR analysis of neuronal markers in neurons. Run data were analyzed by the ΔΔCt method; cDNA from qPCR Human Reference Total RNA was used as a calibrator and β-ACTIN expression was used for normalization. Data are expressed as fold increases compared with levels in 201B7. *P < 0.05, **P < 0.01, and ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM). (C) Representative images of immunostaining for neuronal markers. Membrane-permeabilized neurons were stained with anti-TUJ1 antibody (green), anti-MAP2 antibody (white), and DAPI (blue). Scale bar = 100 μm. (D) Representative analysis images of TUJ1-positive neurites and DAPI-positive nuclei (scale bar = 200 μm). Neuronal network density was determined as TUJ1-positive %area divided by the number of DAPI-positive cell nuclei (the two latter variables were quantified using FIJI). **P < 0.01, ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM).

Journal: Molecular genetics and metabolism reports

Article Title: A neuropathological cell model derived from Niemann-Pick disease type C patient-specific iPSCs shows disruption of the p62/SQSTM1-KEAP1-NRF2 Axis and impaired formation of neuronal networks.

doi: 10.1016/j.ymgmr.2021.100784

Figure Lengend Snippet: Fig. 4. NPC patient-derived neurons show reduced neuronal network density. (A) Protocol for differentiation of iPSCs-derived NSCs into neurons. (B) qPCR analysis of neuronal markers in neurons. Run data were analyzed by the ΔΔCt method; cDNA from qPCR Human Reference Total RNA was used as a calibrator and β-ACTIN expression was used for normalization. Data are expressed as fold increases compared with levels in 201B7. *P < 0.05, **P < 0.01, and ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM). (C) Representative images of immunostaining for neuronal markers. Membrane-permeabilized neurons were stained with anti-TUJ1 antibody (green), anti-MAP2 antibody (white), and DAPI (blue). Scale bar = 100 μm. (D) Representative analysis images of TUJ1-positive neurites and DAPI-positive nuclei (scale bar = 200 μm). Neuronal network density was determined as TUJ1-positive %area divided by the number of DAPI-positive cell nuclei (the two latter variables were quantified using FIJI). **P < 0.01, ***P < 0.001 (ANOVA followed by Tukey’s multiple comparisons test; N = 4, mean ± SEM).

Article Snippet: From this total RNA, 500 ng was used for reverse transcription, which was achieved using ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO Co. Ltd., Osaka, Japan).

Techniques: Derivative Assay, Expressing, Immunostaining, Membrane, Staining

QRT-PCR Validation of Gene Array Results for Baboon Renal Cortex

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: A Custom Rat and Baboon Hypertension Gene Array to Compare Experimental Models

doi: 10.1258/ebm.2011.011188

Figure Lengend Snippet: QRT-PCR Validation of Gene Array Results for Baboon Renal Cortex

Article Snippet: We quantified mRNA according to manufacturer’s instructions (Applied Biosystems Inc., Foster City, CA: ACE1 , Hs00174179_m1; GAL , Hs00544355_m1; IGF1 , Hs00153126_m1).

Techniques: Biomarker Discovery