Journal:

Article Title: New Simple and Rapid Test for Culture Confirmation of Mycobacterium tuberculosis Complex: a Multicenter Study

doi: 10.1128/JCM.40.3.908-912.2002

Figure Lengend Snippet: Sequential evaluation of sputum cultures prior to and after antituberculosis treatment a

Article Snippet: As a reference identification method, all of the cultures tested in this study were identified using either with the AccuProbe culture confirmation kits (Gen-Probe, San Diego, Calif.), by the DNA-DNA hybridization method (Kyokuto Pharmaceutical Co., Ltd., Tokyo, Japan) ( 7 ) (site 1), by high-pressure liquid chromatography, or by biochemical testing.

Techniques:

Journal:

Article Title: False-Positive Results for Mycobacterium celatum with the AccuProbe Mycobacterium tuberculosis Complex Assay

doi:

Figure Lengend Snippet: (A) Effect of selection time on RLU value in the AccuProbe M. tuberculosis complex assay with M. celatum. There were no statistically significant differences between the mean RLU values with solid or liquid medium for the different selection times. The RLU values from the solid medium suspension were significantly higher than those from the broth-grown suspension at all selection times (P < 0.05). Data are expressed as means plus SEM. The RLU cutoff is 30,000. (B) Effect of temperature on RLU value in the AccuProbe M. tuberculosis complex assay with M. celatum. The Mann-Whitney U test revealed significant differences between the mean RLU values with solid medium for 58°C versus 60 and 61°C, between 59°C versus 60 and 61°C, and 60°C versus 61°C (P < 0.05). The RLU values from the solid medium suspension were significantly higher than those from the broth-grown suspension at all selection temperatures (P < 0.05). Data are expressed as means plus SEM. The RLU cutoff is indicated by a horizontal line.

Article Snippet: These results indicate that in spite of Gen-Probe's previous modification of the selection time from 5 to 10 min ( 16 ; 1993 original and 1994 revised package inserts for the AccuProbe cultural identification test, Gen-Probe Incorporated), a considerable number of laboratories still obtained false-positive TB AccuProbe results with M. celatum .

Techniques: Selection, MANN-WHITNEY