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Image Search Results
Journal: Journal of Inflammation Research
Article Title: Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats
doi: 10.2147/JIR.S367193
Figure Lengend Snippet: TRPV1 and TRPA1 expression in vulvar nerves after multiple rounds of zymosan challenge. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 20 days of the 3rd round (n=8 per group). Mean ± SEM. * P <0.05, ** P <0.005, *** P <0.001.
Article Snippet: Afterward, to remove background staining, we used Background Buster (#NB306-50, INNOVEX), followed by three cycles of wash buffer for two minutes each, then incubated for one hour at room temperature in a humidity chamber with one of the first antibodies:
Techniques: Expressing, Saline, Staining, Fluorescence
Journal: Journal of Inflammation Research
Article Title: Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats
doi: 10.2147/JIR.S367193
Figure Lengend Snippet: TRPV1 and TRPA1 expression in vulvar nerves after 160 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 20 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; White arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 20 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; White arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the zymosan and the saline groups 160 days of the 3rd round (n=8 per group). Mean ± SEM. *** P <0.001.
Article Snippet: Afterward, to remove background staining, we used Background Buster (#NB306-50, INNOVEX), followed by three cycles of wash buffer for two minutes each, then incubated for one hour at room temperature in a humidity chamber with one of the first antibodies:
Techniques: Expressing, Saline, Staining, Fluorescence
Journal: Journal of Inflammation Research
Article Title: Characterization of Early Inflammatory Events Leading to Provoked Vulvodynia Development in Rats
doi: 10.2147/JIR.S367193
Figure Lengend Snippet: Long-term effects of mast cells stabilizer KF on TRPV1 and TRPA1 expression in vulvar nerves after 81 days of the 3rd round of zymosan/saline administration. ( A ) The expression of TRPV1 channel in vulva nerves, 81 days after the 3rd zymosan/saline administration. Coexpression of TRPV1 channel (green) and neuronal PGP 9.5 (red; white arrows) merged with dapi stain (blue). ( B ) The expression of TRPA1 channel in vulva nerves, 81 days after the 3rd round zymosan\saline administration. Coexpression of TRPA1 channel (red) and neuronal PGP 9.5 (green; white arrows) merged with dapi stain (blue). Scale bar: 50 µm. ( C ) Fluorescence intensity (arbitrary units) of TRPV1, TRPA1, TRPV1/PGP-9.5, and TRPA1/PGP-9.5 in the Saline- ketotifen, the zymosan- ketotifen, and the zymosan-saline group 81 of the 3rd round (n=7 per group). Mean ± SEM. * P <0.05, *** P <0.001.
Article Snippet: Afterward, to remove background staining, we used Background Buster (#NB306-50, INNOVEX), followed by three cycles of wash buffer for two minutes each, then incubated for one hour at room temperature in a humidity chamber with one of the first antibodies:
Techniques: Expressing, Saline, Staining, Fluorescence