Journal:

Article Title: Farnesyltransferase inhibitors induce cytochrome c release and caspase 3 activation preferentially in transformed cells

doi:

Figure Lengend Snippet: FTI induces activation of caspase 3 but not caspase 1. KNRK cells were treated with DMSO (control), 20 μM PD98059 (MEK inhibitor), 20 μM SCH56582 (FTI), or 20 μM SCH56582 plus 50 mM Z-DEVD-fmk (FTI + Z-DEVD-fmk) in 0.1% FCS medium. Cells were collected after 24 h, and caspase 3 and caspase 1 activities were measured as described in Materials and Methods by using the substrates Ac-DEVD-AMC and Ac-YVAD-AMC, respectively. At least 10,000 cells were examined in each assay.

Article Snippet: The caspase 1 assay was carried out similarly using the substrate Ac-YVAD-AMC (PharMingen).

Techniques: Activation Assay

Journal:

Article Title: Conserved Cysteine-Rich Domain of Paramyxovirus Simian Virus 5 V Protein Plays an Important Role in Blocking Apoptosis

doi: 10.1128/JVI.78.10.5068-5078.2004

Figure Lengend Snippet: Caspase activities of mock-, SV5-, and rSV5VΔC-infected cells a

Article Snippet: Similarly, subsequent caspase assays were carried out using 20 μM Ac-YVAD-AMC substrate (BD Bioscience) for caspase 1, 50 μM Ac-LEVD-AMC substrate (A.G. Scientific Inc., San Diego, Calif.) for caspase 4, 50 μM Ac-LEHD-AMC substrate (A.G. Scientific Inc.) for caspases 5, 9, 10, and 11, 50 μM Ac-VEID-AMC substrate (A. G. Scientific Inc.) for caspase 6 assay, 20 μM Ac-LETD-AMC substrate (BD Bioscience) for caspase 8, and 100 μM Ac-LEED-AFC substrate (Enzymesys, Aurora, Ohio) for caspase 13.

Techniques: Sequencing, Infection

Journal:

Article Title: Farnesyltransferase inhibitors induce cytochrome c release and caspase 3 activation preferentially in transformed cells

doi:

Figure Lengend Snippet: FTI induces activation of caspase 3 but not caspase 1. KNRK cells were treated with DMSO (control), 20 μM PD98059 (MEK inhibitor), 20 μM SCH56582 (FTI), or 20 μM SCH56582 plus 50 mM Z-DEVD-fmk (FTI + Z-DEVD-fmk) in 0.1% FCS medium. Cells were collected after 24 h, and caspase 3 and caspase 1 activities were measured as described in Materials and Methods by using the substrates Ac-DEVD-AMC and Ac-YVAD-AMC, respectively. At least 10,000 cells were examined in each assay.

Article Snippet: The caspase-3 fluorogenic substrate, Ac-DEVD-AMC, and caspase-1 fluorogenic substrate, Ac-YVAD-AMC, were purchased from PharMingen (AMC, 7-amino-4-methylcoumarin).

Techniques: Activation Assay