Journal: Hepatology Communications

Article Title: Constitutive Androstane Receptor Differentially Regulates Bile Acid Homeostasis in Mouse Models of Intrahepatic Cholestasis

doi: 10.1002/hep4.1274

Figure Lengend Snippet: TKO further attenuates ABCB11‐mediated fecal BA excretion. (A) mRNA (n = 8) and (B) protein levels (n = 3) of ABCB11 were analyzed by q‐PCR and proteomics, respectively. Ratio (%) indicates the proportion of ABCB11 expressions compared to WT. (C) Immunostaining of ABCB11 (magnification ×200); arrowhead, residual canalicular ABCB11 expression. (D) BA levels in gallbladder‐collected bile (n = 4) and feces (n = 3). ANOVA followed by Tukey HSD, # P < 0.05, ### P < 0.005. Abbreviation: iFOT, intensity‐based fraction of total.

Article Snippet: Briefly, after antigen retrieval in sodium citrate buffer (pH 6.0), sections were incubated with blocking buffer (5% normal goat serum, 30 minutes) followed by diluted anti‐KRT19 (1:200, Ab133496; Abcam; Research Resource Identifier [RRID], AB_11155282), anti‐ABCB11 (1:100, R31844; NSJ Bioreagents, San Diego, CA; RRID, AB_2725751), anti‐ABCC3 (1:20, sc‐59612; Santa Cruz Biotechnology, Dallas, TX; RRID, AB_631970), and anti‐ABCC4 (1:50, sc‐376262; Santa Cruz Biotechnology; RRID, AB_10987858) antibodies at room temperature for 2 hours.

Techniques: Immunostaining, Expressing

Journal: Hepatology Communications

Article Title: Constitutive Androstane Receptor Differentially Regulates Bile Acid Homeostasis in Mouse Models of Intrahepatic Cholestasis

doi: 10.1002/hep4.1274

Figure Lengend Snippet: TC treatment enhances canalicular and basolateral BA transporters. (A) Fecal BA excretion (n = 4) and urinary BA concentration (n = 3) in TC‐treated (1 week) DKO and BKO. (B) mRNA levels of BA transporters (n = 4). (C) Immunostaining of ABCB11, ABCC3, and ABCC4 (magnification ×200). Arrowhead, positive area. Student t test; * P < 0.05, *** P < 0.005 compared to CO‐treated group. Abbreviation: MDR, multidrug resistance.

Article Snippet: Briefly, after antigen retrieval in sodium citrate buffer (pH 6.0), sections were incubated with blocking buffer (5% normal goat serum, 30 minutes) followed by diluted anti‐KRT19 (1:200, Ab133496; Abcam; Research Resource Identifier [RRID], AB_11155282), anti‐ABCB11 (1:100, R31844; NSJ Bioreagents, San Diego, CA; RRID, AB_2725751), anti‐ABCC3 (1:20, sc‐59612; Santa Cruz Biotechnology, Dallas, TX; RRID, AB_631970), and anti‐ABCC4 (1:50, sc‐376262; Santa Cruz Biotechnology; RRID, AB_10987858) antibodies at room temperature for 2 hours.

Techniques: Concentration Assay, Immunostaining

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Human PXR Signaling Exacerbates Ethanol-induced Liver Injury in Females: Evidence From Mouse Models and Human Alcohol-associated Liver Disease Cohorts

doi: 10.1016/j.jcmgh.2025.101589

Figure Lengend Snippet: Sex-specific regulation of CAR, FXR, and ATP-binding cassette genes in response to ethanol. Total RNA was extracted from frozen liver obtained and evaluated for changes in mRNA expression of the following genes by RT-qPCR relative to the housekeeping Gapdh mRNA : ( A ) Esr1 ; ( B ) Car ; ( C ) Cyp2b10 ; ( D ) Cyp2b9 ; ( E ) Cyp2b13 ; ( F ) Fxr ; ( G ) Cyp7a1 ; ( H ) Abcb11 ; ( I ) Abcb4 ; ( J ) Abcg8 ; and ( K ) Abcc3 . Frozen liver homogenates were evaluated for changes in expression of the following proteins by Western blot relative to the housekeeping protein α-tubulin: ( L ) CAR protein for WT; ( M ) CAR protein for hPXR; ( N ) CYP2B10 protein for WT; ( O ) CYP2B10 protein for hPXR; ( P ) CYP2E1 for WT; ( Q ) CYP2E1 for hPXR; ( R ) FXR for WT; ( S ) FXR for hPXR. The membrane used to probe for CAR protein expression in L was stripped and reprobed with primary antibodies against FXR in R and M . Data represents mean ± SEM (n = 6). ∗ P ≤ .05, ∗∗ P ≤ .01, ∗∗∗ P ≤ .001, ∗∗∗∗ P ≤ .0001 between indicated groups.

Article Snippet: ATP binding cassette subfamily B member 11 (Abcb11) , Mm00445168_m1.

Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Western Blot, Membrane