abcb11 Search Results


gene exp rara hs00940446 m1  (Thermo Fisher)
91
Thermo Fisher gene exp rara hs00940446 m1
Gene Exp Rara Hs00940446 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti abcb11  (NSJ Bioreagents)
94
NSJ Bioreagents anti abcb11
TKO further attenuates <t>ABCB11‐mediated</t> fecal BA excretion. (A) mRNA (n = 8) and (B) protein levels (n = 3) of ABCB11 were analyzed by q‐PCR and proteomics, respectively. Ratio (%) indicates the proportion of ABCB11 expressions compared to WT. (C) Immunostaining of ABCB11 (magnification ×200); arrowhead, residual canalicular ABCB11 expression. (D) BA levels in gallbladder‐collected bile (n = 4) and feces (n = 3). ANOVA followed by Tukey HSD, # P < 0.05, ### P < 0.005. Abbreviation: iFOT, intensity‐based fraction of total.
Anti Abcb11, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp abcb11 hs00184824 m1  (Thermo Fisher)
87
Thermo Fisher gene exp abcb11 hs00184824 m1
TKO further attenuates <t>ABCB11‐mediated</t> fecal BA excretion. (A) mRNA (n = 8) and (B) protein levels (n = 3) of ABCB11 were analyzed by q‐PCR and proteomics, respectively. Ratio (%) indicates the proportion of ABCB11 expressions compared to WT. (C) Immunostaining of ABCB11 (magnification ×200); arrowhead, residual canalicular ABCB11 expression. (D) BA levels in gallbladder‐collected bile (n = 4) and feces (n = 3). ANOVA followed by Tukey HSD, # P < 0.05, ### P < 0.005. Abbreviation: iFOT, intensity‐based fraction of total.
Gene Exp Abcb11 Hs00184824 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bsep rabbit proteintech  (Proteintech)
94
Proteintech bsep rabbit proteintech
TKO further attenuates <t>ABCB11‐mediated</t> fecal BA excretion. (A) mRNA (n = 8) and (B) protein levels (n = 3) of ABCB11 were analyzed by q‐PCR and proteomics, respectively. Ratio (%) indicates the proportion of ABCB11 expressions compared to WT. (C) Immunostaining of ABCB11 (magnification ×200); arrowhead, residual canalicular ABCB11 expression. (D) BA levels in gallbladder‐collected bile (n = 4) and feces (n = 3). ANOVA followed by Tukey HSD, # P < 0.05, ### P < 0.005. Abbreviation: iFOT, intensity‐based fraction of total.
Bsep Rabbit Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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abcb11 pb9414  (Boster Bio)
94
Boster Bio abcb11 pb9414
TKO further attenuates <t>ABCB11‐mediated</t> fecal BA excretion. (A) mRNA (n = 8) and (B) protein levels (n = 3) of ABCB11 were analyzed by q‐PCR and proteomics, respectively. Ratio (%) indicates the proportion of ABCB11 expressions compared to WT. (C) Immunostaining of ABCB11 (magnification ×200); arrowhead, residual canalicular ABCB11 expression. (D) BA levels in gallbladder‐collected bile (n = 4) and feces (n = 3). ANOVA followed by Tukey HSD, # P < 0.05, ### P < 0.005. Abbreviation: iFOT, intensity‐based fraction of total.
Abcb11 Pb9414, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp abcb11 mm00445168 m1  (Thermo Fisher)
97
Thermo Fisher gene exp abcb11 mm00445168 m1
Western blot and densitometric analysis of transporter protein expression in total liver membrane of WT and Bcrp KO mice. Representative blots (n = 3) are shown, and densitometry values for all six mice are shown below the blots. Expression of apical Mrp2 is down equally in WT and KO mice after BDL. There was no difference in <t>Bsep</t> expression levels after BDL in either group. However, Mrp4 and Mdr1a were up-regulated after BDL in WT and KO mice. The increase in Mrp4 in the KO mice was only half that seen in the WT BDL mice, whereas the increase in Mdr1a was twice as great in the KO mice compared with that in the WT mice. Oatp1, Ntcp, and Oct1 protein expression was significantly decreased after BDL in the livers of both WT and KO animals, although the decrease for Oct1 was significantly less in the Bcrp KO mice than that seen in the WT mice. *, p < 0.05, sham versus BDL; #, p < 0.05, WT BDL versus KO BDL; +, p < 0.05, WT sham versus KO sham.
Gene Exp Abcb11 Mm00445168 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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variants abcb11 rs11568372 p e297g  (Thermo Fisher)
86
Thermo Fisher variants abcb11 rs11568372 p e297g
Western blot and densitometric analysis of transporter protein expression in total liver membrane of WT and Bcrp KO mice. Representative blots (n = 3) are shown, and densitometry values for all six mice are shown below the blots. Expression of apical Mrp2 is down equally in WT and KO mice after BDL. There was no difference in <t>Bsep</t> expression levels after BDL in either group. However, Mrp4 and Mdr1a were up-regulated after BDL in WT and KO mice. The increase in Mrp4 in the KO mice was only half that seen in the WT BDL mice, whereas the increase in Mdr1a was twice as great in the KO mice compared with that in the WT mice. Oatp1, Ntcp, and Oct1 protein expression was significantly decreased after BDL in the livers of both WT and KO animals, although the decrease for Oct1 was significantly less in the Bcrp KO mice than that seen in the WT mice. *, p < 0.05, sham versus BDL; #, p < 0.05, WT BDL versus KO BDL; +, p < 0.05, WT sham versus KO sham.
Variants Abcb11 Rs11568372 P E297g, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp abcb11 hs00184829 m1  (Thermo Fisher)
85
Thermo Fisher gene exp abcb11 hs00184829 m1
Western blot and densitometric analysis of transporter protein expression in total liver membrane of WT and Bcrp KO mice. Representative blots (n = 3) are shown, and densitometry values for all six mice are shown below the blots. Expression of apical Mrp2 is down equally in WT and KO mice after BDL. There was no difference in <t>Bsep</t> expression levels after BDL in either group. However, Mrp4 and Mdr1a were up-regulated after BDL in WT and KO mice. The increase in Mrp4 in the KO mice was only half that seen in the WT BDL mice, whereas the increase in Mdr1a was twice as great in the KO mice compared with that in the WT mice. Oatp1, Ntcp, and Oct1 protein expression was significantly decreased after BDL in the livers of both WT and KO animals, although the decrease for Oct1 was significantly less in the Bcrp KO mice than that seen in the WT mice. *, p < 0.05, sham versus BDL; #, p < 0.05, WT BDL versus KO BDL; +, p < 0.05, WT sham versus KO sham.
Gene Exp Abcb11 Hs00184829 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp abcb11 hs00994805 m1  (Thermo Fisher)
94
Thermo Fisher gene exp abcb11 hs00994805 m1
Western blot and densitometric analysis of transporter protein expression in total liver membrane of WT and Bcrp KO mice. Representative blots (n = 3) are shown, and densitometry values for all six mice are shown below the blots. Expression of apical Mrp2 is down equally in WT and KO mice after BDL. There was no difference in <t>Bsep</t> expression levels after BDL in either group. However, Mrp4 and Mdr1a were up-regulated after BDL in WT and KO mice. The increase in Mrp4 in the KO mice was only half that seen in the WT BDL mice, whereas the increase in Mdr1a was twice as great in the KO mice compared with that in the WT mice. Oatp1, Ntcp, and Oct1 protein expression was significantly decreased after BDL in the livers of both WT and KO animals, although the decrease for Oct1 was significantly less in the Bcrp KO mice than that seen in the WT mice. *, p < 0.05, sham versus BDL; #, p < 0.05, WT BDL versus KO BDL; +, p < 0.05, WT sham versus KO sham.
Gene Exp Abcb11 Hs00994805 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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gene exp abcb11 hs00994811 m1  (Thermo Fisher)
93
Thermo Fisher gene exp abcb11 hs00994811 m1
Western blot and densitometric analysis of transporter protein expression in total liver membrane of WT and Bcrp KO mice. Representative blots (n = 3) are shown, and densitometry values for all six mice are shown below the blots. Expression of apical Mrp2 is down equally in WT and KO mice after BDL. There was no difference in <t>Bsep</t> expression levels after BDL in either group. However, Mrp4 and Mdr1a were up-regulated after BDL in WT and KO mice. The increase in Mrp4 in the KO mice was only half that seen in the WT BDL mice, whereas the increase in Mdr1a was twice as great in the KO mice compared with that in the WT mice. Oatp1, Ntcp, and Oct1 protein expression was significantly decreased after BDL in the livers of both WT and KO animals, although the decrease for Oct1 was significantly less in the Bcrp KO mice than that seen in the WT mice. *, p < 0.05, sham versus BDL; #, p < 0.05, WT BDL versus KO BDL; +, p < 0.05, WT sham versus KO sham.
Gene Exp Abcb11 Hs00994811 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp abcb11 rn00582179 m1  (Thermo Fisher)
85
Thermo Fisher gene exp abcb11 rn00582179 m1
Gene expression of efflux transporters during endotoxemia
Gene Exp Abcb11 Rn00582179 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


TKO further attenuates ABCB11‐mediated fecal BA excretion. (A) mRNA (n = 8) and (B) protein levels (n = 3) of ABCB11 were analyzed by q‐PCR and proteomics, respectively. Ratio (%) indicates the proportion of ABCB11 expressions compared to WT. (C) Immunostaining of ABCB11 (magnification ×200); arrowhead, residual canalicular ABCB11 expression. (D) BA levels in gallbladder‐collected bile (n = 4) and feces (n = 3). ANOVA followed by Tukey HSD, # P < 0.05, ### P < 0.005. Abbreviation: iFOT, intensity‐based fraction of total.

Journal: Hepatology Communications

Article Title: Constitutive Androstane Receptor Differentially Regulates Bile Acid Homeostasis in Mouse Models of Intrahepatic Cholestasis

doi: 10.1002/hep4.1274

Figure Lengend Snippet: TKO further attenuates ABCB11‐mediated fecal BA excretion. (A) mRNA (n = 8) and (B) protein levels (n = 3) of ABCB11 were analyzed by q‐PCR and proteomics, respectively. Ratio (%) indicates the proportion of ABCB11 expressions compared to WT. (C) Immunostaining of ABCB11 (magnification ×200); arrowhead, residual canalicular ABCB11 expression. (D) BA levels in gallbladder‐collected bile (n = 4) and feces (n = 3). ANOVA followed by Tukey HSD, # P < 0.05, ### P < 0.005. Abbreviation: iFOT, intensity‐based fraction of total.

Article Snippet: Briefly, after antigen retrieval in sodium citrate buffer (pH 6.0), sections were incubated with blocking buffer (5% normal goat serum, 30 minutes) followed by diluted anti‐KRT19 (1:200, Ab133496; Abcam; Research Resource Identifier [RRID], AB_11155282), anti‐ABCB11 (1:100, R31844; NSJ Bioreagents, San Diego, CA; RRID, AB_2725751), anti‐ABCC3 (1:20, sc‐59612; Santa Cruz Biotechnology, Dallas, TX; RRID, AB_631970), and anti‐ABCC4 (1:50, sc‐376262; Santa Cruz Biotechnology; RRID, AB_10987858) antibodies at room temperature for 2 hours.

Techniques: Immunostaining, Expressing

TC treatment enhances canalicular and basolateral BA transporters. (A) Fecal BA excretion (n = 4) and urinary BA concentration (n = 3) in TC‐treated (1 week) DKO and BKO. (B) mRNA levels of BA transporters (n = 4). (C) Immunostaining of ABCB11, ABCC3, and ABCC4 (magnification ×200). Arrowhead, positive area. Student t test; * P < 0.05, *** P < 0.005 compared to CO‐treated group. Abbreviation: MDR, multidrug resistance.

Journal: Hepatology Communications

Article Title: Constitutive Androstane Receptor Differentially Regulates Bile Acid Homeostasis in Mouse Models of Intrahepatic Cholestasis

doi: 10.1002/hep4.1274

Figure Lengend Snippet: TC treatment enhances canalicular and basolateral BA transporters. (A) Fecal BA excretion (n = 4) and urinary BA concentration (n = 3) in TC‐treated (1 week) DKO and BKO. (B) mRNA levels of BA transporters (n = 4). (C) Immunostaining of ABCB11, ABCC3, and ABCC4 (magnification ×200). Arrowhead, positive area. Student t test; * P < 0.05, *** P < 0.005 compared to CO‐treated group. Abbreviation: MDR, multidrug resistance.

Article Snippet: Briefly, after antigen retrieval in sodium citrate buffer (pH 6.0), sections were incubated with blocking buffer (5% normal goat serum, 30 minutes) followed by diluted anti‐KRT19 (1:200, Ab133496; Abcam; Research Resource Identifier [RRID], AB_11155282), anti‐ABCB11 (1:100, R31844; NSJ Bioreagents, San Diego, CA; RRID, AB_2725751), anti‐ABCC3 (1:20, sc‐59612; Santa Cruz Biotechnology, Dallas, TX; RRID, AB_631970), and anti‐ABCC4 (1:50, sc‐376262; Santa Cruz Biotechnology; RRID, AB_10987858) antibodies at room temperature for 2 hours.

Techniques: Concentration Assay, Immunostaining

Western blot and densitometric analysis of transporter protein expression in total liver membrane of WT and Bcrp KO mice. Representative blots (n = 3) are shown, and densitometry values for all six mice are shown below the blots. Expression of apical Mrp2 is down equally in WT and KO mice after BDL. There was no difference in Bsep expression levels after BDL in either group. However, Mrp4 and Mdr1a were up-regulated after BDL in WT and KO mice. The increase in Mrp4 in the KO mice was only half that seen in the WT BDL mice, whereas the increase in Mdr1a was twice as great in the KO mice compared with that in the WT mice. Oatp1, Ntcp, and Oct1 protein expression was significantly decreased after BDL in the livers of both WT and KO animals, although the decrease for Oct1 was significantly less in the Bcrp KO mice than that seen in the WT mice. *, p < 0.05, sham versus BDL; #, p < 0.05, WT BDL versus KO BDL; +, p < 0.05, WT sham versus KO sham.

Journal: Drug Metabolism and Disposition

Article Title: Role of Breast Cancer Resistance Protein in the Adaptive Response to Cholestasis

doi: 10.1124/dmd.110.034512

Figure Lengend Snippet: Western blot and densitometric analysis of transporter protein expression in total liver membrane of WT and Bcrp KO mice. Representative blots (n = 3) are shown, and densitometry values for all six mice are shown below the blots. Expression of apical Mrp2 is down equally in WT and KO mice after BDL. There was no difference in Bsep expression levels after BDL in either group. However, Mrp4 and Mdr1a were up-regulated after BDL in WT and KO mice. The increase in Mrp4 in the KO mice was only half that seen in the WT BDL mice, whereas the increase in Mdr1a was twice as great in the KO mice compared with that in the WT mice. Oatp1, Ntcp, and Oct1 protein expression was significantly decreased after BDL in the livers of both WT and KO animals, although the decrease for Oct1 was significantly less in the Bcrp KO mice than that seen in the WT mice. *, p < 0.05, sham versus BDL; #, p < 0.05, WT BDL versus KO BDL; +, p < 0.05, WT sham versus KO sham.

Article Snippet: TaqMan Gene Expression Assays were used for the following: Bcrp ( {"type":"entrez-nucleotide","attrs":{"text":"NM_011920","term_id":"110815847"}} NM_011920 ), Mm01181556_m1; Mrp2 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_013806","term_id":"116063565"}} NM_013806 ), Mm00496899_m1; Mrp3 ( {"type":"entrez-nucleotide","attrs":{"text":"BC046560","term_id":"28279835"}} BC046560 ), Mm00551550_m1; Ntcp ( {"type":"entrez-nucleotide","attrs":{"text":"NM_011387","term_id":"294774570"}} NM_011387 ), Mm00441421_m1; Mdr1a ( {"type":"entrez-nucleotide","attrs":{"text":"NM_011076","term_id":"1315370118"}} NM_011076 ), Mm00440761; and Bsep ( {"type":"entrez-nucleotide","attrs":{"text":"NM_021022","term_id":"120432046"}} NM_021022 ), Mm00445168_m1.

Techniques: Western Blot, Expressing, Membrane

Gene expression of efflux transporters during endotoxemia

Journal: Pflugers Archiv

Article Title: Nitric oxide differentially regulates renal ATP-binding cassette transporters during endotoxemia

doi: 10.1007/s00424-007-0210-x

Figure Lengend Snippet: Gene expression of efflux transporters during endotoxemia

Article Snippet: Abcb11 , Bsep , Rn00582179_m1 , 10.5 ± 0.40 , 8.6 ± 0.37 , 10.3 ± 0.24 , 11.9 ± 0.21 , 8.7 ± 0.17 , 5.4 ± 0.49 , 9.9 ± 0.21 , 9.7 ± 0.21.

Techniques: Gene Expression, Full Display Name

Protein expression of different renal ABC transporters. Total membrane fractions of rat kidney were isolated and expression of Abcb1/P-gp ( a , b ), Abcb11/Bsep ( c ), Abcc2/Mrp2 ( d , e ), and Abcc4/Mrp4 ( f , g ) was determined by Western blotting. Representative images ( n = 4) show maximal Abcb1/P-gp protein expression 12 and 24 h after LPS + ( a ), and coadministration with aminoguanidine reversed this induction ( b ). The protein expression of Abcb11/Bsep was up-regulated 12 h after LPS + treatment, which was reversed by aminoguanidine ( c ). Maximal Abcc2/Mrp2 protein expression was seen 12 h after LPS + ( d ) and aminoguanidine reversed this induction ( e ). Abcc4/Mrp4 protein expression was down-regulated during endotoxemia ( f ), which was intensified by aminoguanidine ( g )

Journal: Pflugers Archiv

Article Title: Nitric oxide differentially regulates renal ATP-binding cassette transporters during endotoxemia

doi: 10.1007/s00424-007-0210-x

Figure Lengend Snippet: Protein expression of different renal ABC transporters. Total membrane fractions of rat kidney were isolated and expression of Abcb1/P-gp ( a , b ), Abcb11/Bsep ( c ), Abcc2/Mrp2 ( d , e ), and Abcc4/Mrp4 ( f , g ) was determined by Western blotting. Representative images ( n = 4) show maximal Abcb1/P-gp protein expression 12 and 24 h after LPS + ( a ), and coadministration with aminoguanidine reversed this induction ( b ). The protein expression of Abcb11/Bsep was up-regulated 12 h after LPS + treatment, which was reversed by aminoguanidine ( c ). Maximal Abcc2/Mrp2 protein expression was seen 12 h after LPS + ( d ) and aminoguanidine reversed this induction ( e ). Abcc4/Mrp4 protein expression was down-regulated during endotoxemia ( f ), which was intensified by aminoguanidine ( g )

Article Snippet: Abcb11 , Bsep , Rn00582179_m1 , 10.5 ± 0.40 , 8.6 ± 0.37 , 10.3 ± 0.24 , 11.9 ± 0.21 , 8.7 ± 0.17 , 5.4 ± 0.49 , 9.9 ± 0.21 , 9.7 ± 0.21.

Techniques: Expressing, Membrane, Isolation, Western Blot

Distribution of Abcb11/Bsep in rat kidney cortex. a , b Representative immunohistochemical images show apical staining in proximal tubules (indicated by arrows ). In addition, glomerular and peritubular staining of Bsep in the cortex was found. G glomerulus, asterisks peritubular staining, P proximal tubule, D distal tubule. Original magnification in a ×200, and in b ×400

Journal: Pflugers Archiv

Article Title: Nitric oxide differentially regulates renal ATP-binding cassette transporters during endotoxemia

doi: 10.1007/s00424-007-0210-x

Figure Lengend Snippet: Distribution of Abcb11/Bsep in rat kidney cortex. a , b Representative immunohistochemical images show apical staining in proximal tubules (indicated by arrows ). In addition, glomerular and peritubular staining of Bsep in the cortex was found. G glomerulus, asterisks peritubular staining, P proximal tubule, D distal tubule. Original magnification in a ×200, and in b ×400

Article Snippet: Abcb11 , Bsep , Rn00582179_m1 , 10.5 ± 0.40 , 8.6 ± 0.37 , 10.3 ± 0.24 , 11.9 ± 0.21 , 8.7 ± 0.17 , 5.4 ± 0.49 , 9.9 ± 0.21 , 9.7 ± 0.21.

Techniques: Immunohistochemical staining, Staining