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Image Search Results
Journal: Journal for Immunotherapy of Cancer
Article Title: Induction of EBV latent membrane protein-2A (LMP2A)-specific T cells and construction of individualized TCR-engineered T cells for EBV-associated malignancies
doi: 10.1136/jitc-2021-002516
Figure Lengend Snippet: Phenotype and functional screening of S0 and S2 in each donor. (A) Flow cytometry analysis revealed percentages of CD4+ and CD8+ T cells. (B) Flow cytometry analysis revealed percentages of naive, effect, effect memory (TEM) and central memory T cells (TCM). (C) IFN-γ ELISA analysis of S2 T cells cocultured autologous DCs pulsed with LMP2A peptides. (D) IFN-γ ELISPOT analysis of S2 T cells cocultured autologous DCs pulsed with LMP2A peptides or not. Column histogram summarized the number of positive spots (E). The mean values from each group are plotted. Error bars represent the SEM (**p<0.01, ns, not significant, analyzed by one-way analysis of variance). NC, unstimulated peripheral T cells of each donor.
Article Snippet:
Techniques: Functional Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot
Journal: Journal for Immunotherapy of Cancer
Article Title: Induction of EBV latent membrane protein-2A (LMP2A)-specific T cells and construction of individualized TCR-engineered T cells for EBV-associated malignancies
doi: 10.1136/jitc-2021-002516
Figure Lengend Snippet: Functional assay of potential LMP2A-reactive TCR-Ts. (A) IFN-γ ELISA assay on TCR-Ts cocultured with DCs loaded with LMP2A peptides. (B) IFN-γ ELISPOT assay on TCR-Ts cocultured with DCs pulsed with LMP2A peptides. (C) CD25 and CD137 staining on TCR-Ts cocultured with DCs loaded with LMP2A peptides. Error bars represent the SEM (*p<0.05, **p<0.01, ***p<0.001, ns, not significant, analyzed by one-way analysis of variance). LMP2A, latent membrane protein-2A; NC, untransduced T cells.
Article Snippet:
Techniques: Functional Assay, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Staining, Membrane