Journal: Frontiers in Pharmacology

Article Title: Yi-Shen-Hua-Shi granules inhibit diabetic nephropathy by ameliorating podocyte injury induced by macrophage-derived exosomes

doi: 10.3389/fphar.2022.962606

Figure Lengend Snippet: Identification of macrophage exosomes and the effects of YSHS and high-glucose stimulation on macrophage-derived exosome secretion. (A) Images of macrophage-derived exosome morphology taken by transmission electron microscopy (TEM). (B) Measurement of the protein level of CD9, CD63, and CD81 of macrophage-derived exosomes by Western blotting. (C) The protein concentration of macrophage-derived exosomes was detected by the BCA method. Data analysis adopts a one-way ANOVA analysis of variance. SD indicates error bars ns p > 0.05. NG indicates normal glucose (5.5 mM d -glucose), and HG indicates high glucose (30 mM d -glucose). Con-NG-RAW264.7-exo indicates exosomes from macrophage RAW264.7 cells treated with normal glucose and 10% control serum, Con-HG-RAW264.7-exo represents exosomes from macrophage RAW264.7 cells treated with high glucose and 10% control serum, and YSHS-HG-RAW264.7-exo represents exosomes from macrophage RAW264.7 cells treated with high glucose and 10% YSHS-serum.

Article Snippet: After blocking with 4% milk containing 0.1% Tween, add primary anti-CD9 antibody (1:2000; Abcam, ab92726), CD63 antibody (1:2000; Abcam, ab216130), CD81 antibody (1:1000; Abcam, ab155760), caspase-3 antibody (1:2000; Abcam, ab184787), GAPDH antibody (1:2500; Abcam, ab9485), and incubate overnight at 4°C.

Techniques: Derivative Assay, Transmission Assay, Electron Microscopy, Western Blot, Protein Concentration, Control

Journal: Scientific reports

Article Title: Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors.

doi: 10.1038/s41598-022-27176-w

Figure Lengend Snippet: Figure 1. Characteristics of SVF-EVs. (A) TEM images of SVF-EVs. (B) NTA analysis of SVF-EVs. The confirmed size distribution with a range of diameter around 100–300 nm. (C) Western blot shows that SVF- EVs express tetraspanin proteins like TSG101 and CD81. (D) Western blot reveals that SVF-EVs encapsulate proteins such as PPARγ, C/EBPα, Ang-1, and VEGF. (E) Confocal images verify the internalization of PKH26- labeled SVF-EVs (red) into endothelial cell (CD31-labeled, green), and the amount of endocytosed SVF-EVs after co-culturing for 4 h is more than that for 2 h (F).

Article Snippet: Then membranes were blocked with 5% skimmed milk, and incubated with specific antibodies against TSG101 (ab133586, Abcam, UK), CD81(ab109201, Abcam, UK), PPARγ (sc-7273, Santa, USA), CEBPα (sc-365318, Santa, USA), Ang-1 (23302-1-AP, Sanying, China) and VEGF (66828-1-Ig, Sanying, China) overnight at 4 °C.

Techniques: Western Blot, Labeling

Journal: PLoS ONE

Article Title: Mesenchymal Stem Cells Stabilize Atherosclerotic Vulnerable Plaque by Anti-Inflammatory Properties

doi: 10.1371/journal.pone.0136026

Figure Lengend Snippet: Confirmation of MSC identity by flow cytometry analysis. Cells were positive for CD29 (99.7%), CD44 (98.8%) and CD90 (98.5%) and showed low expression of CD14 (0.3%), and CD45 (0.4%). The black lines are isotype controls.

Article Snippet: MSCs were assessed by flow cytometry analysis for the markers CD29 (Abcam, Cambridge, MA), CD44 (AbD Serotec, Kidlington, UK), CD90 (Abcam), CD14 (Abcam) and CD45 (AbD Serotec) according to standard protocols [ ].

Techniques: Flow Cytometry, Expressing