Journal: Cell Transplantation

Article Title: Intravenous transplantation of olfactory bulb ensheathing cells for a spinal cord hemisection injury rat model

doi: 10.1177/0963689719883842

Figure Lengend Snippet: Exploration of the effect of OECs on axonal sprouting in the spinal cord after SCI. (a) GAP-43 immunostaining in the SCI/OEC, SCI/QD, and control groups at one day, three days, seven days, and 28 days after transplantation. (b) Average staining intensity (integrated optical density, IOD) of GAP-43 positive cells. Data are presented as mean ± SD, n = 5 per group. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; scale bar = 100 μm, applicable to all sections.

Article Snippet: Sections were incubated with the following primary antibodies: anti-Myelin Basic Protein (MBP) antibody (1:50, Abcam, ab7349, Cambridge, MA, USA), anti-Iba-1 antibody (1:50, Abcam, ab139590, Cambridge, MA, USA), anti-GAP-43 antibody (1:500, Abcam, ab16053, Cambridge, MA, USA) and anti-p75 nerve growth factor receptor (NGFR) antibody (1:500, Abcam, ab7349, Cambridge, MA, USA).

Techniques: Immunostaining, Transplantation Assay, Staining

Journal: Research in Pharmaceutical Sciences

Article Title: Inhibition of P-glycoprotein expression and function by anti-diabetic drugs gliclazide, metformin, and pioglitazone in vitro and in situ

doi:

Figure Lengend Snippet: Western blot of P-gp and β- actin in gliclazide (500 μM), metformin (7000 μM), pioglitazone (300 μM), and verapamil (300 μM) treated, and control (non-treated) Caco-2 cells (bands of 2 samples of each are shown).

Article Snippet: The proteins were visualized by exposing the membrane to medical x-ray film (Fuji, Japan) for 5 min in a dark room. β-actin was considered as the internal standard and was detected using rabbit polyclonal anti-β-actin (AB16039, Abcam) as the primary antibody and HRP-conjugated goat anti-rabbit IgG (AP7181, Razi Biotech) as the secondary antibody.

Techniques: Western Blot