ab15537 Search Results


98
Abcam rabbit polyclonal antibody
Rabbit Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech ab15537
Ab15537, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Danaher Inc antibody goat anti rabbit 594 ab15537 abcam
Antibody Goat Anti Rabbit 594 Ab15537 Abcam, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Danaher Inc apc tim 3
Glucose metabolism of CD8 + T cells is impaired in the TME of NPC (A) ECAR of the activated CD8 + T cells after 24 h of culture in normal medium, CM of c6661 cells, and CM along with 2 mg/mL glucose, respectively. (B) Protein levels of glycolysis-related HK2 and PKM2 in CD8 + T cells detected by immunoblot analysis. (C) Apoptosis of CD8 + T cells determined by flow cytometry (FITC and PI labeling). (D) Proliferation of CD8 + T cells determined by flow cytometry (CFSE labeling). (E) Protein level of the cytotoxic marker GZMB in CD8 + T cells detected by immunoblot analysis. (F and G) Proportions of IFN-γ + (F) and TNF-α + (G) CD8 + T cells analyzed by flow cytometry. (H and I) Proportions of <t>TIM-3</t> + (E) and PD-1 + (F) CD8 + T cells analyzed by flow cytometry. (J) Viability of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined using CCK-8 assay. (K) Apoptosis of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined by flow cytometry (FITC and PI labeling). Differences were compared by one-way (C–K) or two-way (A and B) ANOVA. ∗ vs. Normal medium; # vs. CM. ∗#p < 0.05. Three biological replicates were performed. The error bar denotes the standard deviation.
Apc Tim 3, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Danaher Inc cd34 apc
Glucose metabolism of CD8 + T cells is impaired in the TME of NPC (A) ECAR of the activated CD8 + T cells after 24 h of culture in normal medium, CM of c6661 cells, and CM along with 2 mg/mL glucose, respectively. (B) Protein levels of glycolysis-related HK2 and PKM2 in CD8 + T cells detected by immunoblot analysis. (C) Apoptosis of CD8 + T cells determined by flow cytometry (FITC and PI labeling). (D) Proliferation of CD8 + T cells determined by flow cytometry (CFSE labeling). (E) Protein level of the cytotoxic marker GZMB in CD8 + T cells detected by immunoblot analysis. (F and G) Proportions of IFN-γ + (F) and TNF-α + (G) CD8 + T cells analyzed by flow cytometry. (H and I) Proportions of <t>TIM-3</t> + (E) and PD-1 + (F) CD8 + T cells analyzed by flow cytometry. (J) Viability of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined using CCK-8 assay. (K) Apoptosis of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined by flow cytometry (FITC and PI labeling). Differences were compared by one-way (C–K) or two-way (A and B) ANOVA. ∗ vs. Normal medium; # vs. CM. ∗#p < 0.05. Three biological replicates were performed. The error bar denotes the standard deviation.
Cd34 Apc, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Danaher Inc anti human pe cy5 cd80 antibody
Glucose metabolism of CD8 + T cells is impaired in the TME of NPC (A) ECAR of the activated CD8 + T cells after 24 h of culture in normal medium, CM of c6661 cells, and CM along with 2 mg/mL glucose, respectively. (B) Protein levels of glycolysis-related HK2 and PKM2 in CD8 + T cells detected by immunoblot analysis. (C) Apoptosis of CD8 + T cells determined by flow cytometry (FITC and PI labeling). (D) Proliferation of CD8 + T cells determined by flow cytometry (CFSE labeling). (E) Protein level of the cytotoxic marker GZMB in CD8 + T cells detected by immunoblot analysis. (F and G) Proportions of IFN-γ + (F) and TNF-α + (G) CD8 + T cells analyzed by flow cytometry. (H and I) Proportions of <t>TIM-3</t> + (E) and PD-1 + (F) CD8 + T cells analyzed by flow cytometry. (J) Viability of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined using CCK-8 assay. (K) Apoptosis of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined by flow cytometry (FITC and PI labeling). Differences were compared by one-way (C–K) or two-way (A and B) ANOVA. ∗ vs. Normal medium; # vs. CM. ∗#p < 0.05. Three biological replicates were performed. The error bar denotes the standard deviation.
Anti Human Pe Cy5 Cd80 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Danaher Inc cd73 apc
Glucose metabolism of CD8 + T cells is impaired in the TME of NPC (A) ECAR of the activated CD8 + T cells after 24 h of culture in normal medium, CM of c6661 cells, and CM along with 2 mg/mL glucose, respectively. (B) Protein levels of glycolysis-related HK2 and PKM2 in CD8 + T cells detected by immunoblot analysis. (C) Apoptosis of CD8 + T cells determined by flow cytometry (FITC and PI labeling). (D) Proliferation of CD8 + T cells determined by flow cytometry (CFSE labeling). (E) Protein level of the cytotoxic marker GZMB in CD8 + T cells detected by immunoblot analysis. (F and G) Proportions of IFN-γ + (F) and TNF-α + (G) CD8 + T cells analyzed by flow cytometry. (H and I) Proportions of <t>TIM-3</t> + (E) and PD-1 + (F) CD8 + T cells analyzed by flow cytometry. (J) Viability of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined using CCK-8 assay. (K) Apoptosis of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined by flow cytometry (FITC and PI labeling). Differences were compared by one-way (C–K) or two-way (A and B) ANOVA. ∗ vs. Normal medium; # vs. CM. ∗#p < 0.05. Three biological replicates were performed. The error bar denotes the standard deviation.
Cd73 Apc, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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N/A
CD73 NT5E antibody N terminal region Purified Rabbit Polyclonal Antibody Pab
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N/A
This antibody reacts with cluster2membrane protein 125 145 KDa Store at 4°C Do not freeze http www creative diagnostics com Anti Lung carcinom Cluster 1 Antibody 96908 144 htm
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Image Search Results


Glucose metabolism of CD8 + T cells is impaired in the TME of NPC (A) ECAR of the activated CD8 + T cells after 24 h of culture in normal medium, CM of c6661 cells, and CM along with 2 mg/mL glucose, respectively. (B) Protein levels of glycolysis-related HK2 and PKM2 in CD8 + T cells detected by immunoblot analysis. (C) Apoptosis of CD8 + T cells determined by flow cytometry (FITC and PI labeling). (D) Proliferation of CD8 + T cells determined by flow cytometry (CFSE labeling). (E) Protein level of the cytotoxic marker GZMB in CD8 + T cells detected by immunoblot analysis. (F and G) Proportions of IFN-γ + (F) and TNF-α + (G) CD8 + T cells analyzed by flow cytometry. (H and I) Proportions of TIM-3 + (E) and PD-1 + (F) CD8 + T cells analyzed by flow cytometry. (J) Viability of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined using CCK-8 assay. (K) Apoptosis of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined by flow cytometry (FITC and PI labeling). Differences were compared by one-way (C–K) or two-way (A and B) ANOVA. ∗ vs. Normal medium; # vs. CM. ∗#p < 0.05. Three biological replicates were performed. The error bar denotes the standard deviation.

Journal: iScience

Article Title: EP300 restores the glycolytic activity and anti-tumor function of CD8 + cytotoxic T cells in nasopharyngeal carcinoma

doi: 10.1016/j.isci.2024.108957

Figure Lengend Snippet: Glucose metabolism of CD8 + T cells is impaired in the TME of NPC (A) ECAR of the activated CD8 + T cells after 24 h of culture in normal medium, CM of c6661 cells, and CM along with 2 mg/mL glucose, respectively. (B) Protein levels of glycolysis-related HK2 and PKM2 in CD8 + T cells detected by immunoblot analysis. (C) Apoptosis of CD8 + T cells determined by flow cytometry (FITC and PI labeling). (D) Proliferation of CD8 + T cells determined by flow cytometry (CFSE labeling). (E) Protein level of the cytotoxic marker GZMB in CD8 + T cells detected by immunoblot analysis. (F and G) Proportions of IFN-γ + (F) and TNF-α + (G) CD8 + T cells analyzed by flow cytometry. (H and I) Proportions of TIM-3 + (E) and PD-1 + (F) CD8 + T cells analyzed by flow cytometry. (J) Viability of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined using CCK-8 assay. (K) Apoptosis of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined by flow cytometry (FITC and PI labeling). Differences were compared by one-way (C–K) or two-way (A and B) ANOVA. ∗ vs. Normal medium; # vs. CM. ∗#p < 0.05. Three biological replicates were performed. The error bar denotes the standard deviation.

Article Snippet: The proportion of activated CD8 + T cells was screened by the Attune NxT flow cytometer (Thermo Fisher Scientific) using the antibodies of allophycocyanin (APC)-CD3 (ab239287, Abcam), fluorescein isothiocyanate (FITC)-CD8 (ab28010, Abcam), APC-TIM-3 (ab155379, Abcam), APC-PD-1 (ab272338, Abcam) at 4°C overnight.

Techniques: Western Blot, Flow Cytometry, Labeling, Marker, Co-Culture Assay, CCK-8 Assay, Standard Deviation

Journal: iScience

Article Title: EP300 restores the glycolytic activity and anti-tumor function of CD8 + cytotoxic T cells in nasopharyngeal carcinoma

doi: 10.1016/j.isci.2024.108957

Figure Lengend Snippet:

Article Snippet: The proportion of activated CD8 + T cells was screened by the Attune NxT flow cytometer (Thermo Fisher Scientific) using the antibodies of allophycocyanin (APC)-CD3 (ab239287, Abcam), fluorescein isothiocyanate (FITC)-CD8 (ab28010, Abcam), APC-TIM-3 (ab155379, Abcam), APC-PD-1 (ab272338, Abcam) at 4°C overnight.

Techniques: Recombinant, Activation Assay, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Luciferase, Reporter Assay, Sequencing, Software