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Image Search Results
Journal: iScience
Article Title: EP300 restores the glycolytic activity and anti-tumor function of CD8 + cytotoxic T cells in nasopharyngeal carcinoma
doi: 10.1016/j.isci.2024.108957
Figure Lengend Snippet: Glucose metabolism of CD8 + T cells is impaired in the TME of NPC (A) ECAR of the activated CD8 + T cells after 24 h of culture in normal medium, CM of c6661 cells, and CM along with 2 mg/mL glucose, respectively. (B) Protein levels of glycolysis-related HK2 and PKM2 in CD8 + T cells detected by immunoblot analysis. (C) Apoptosis of CD8 + T cells determined by flow cytometry (FITC and PI labeling). (D) Proliferation of CD8 + T cells determined by flow cytometry (CFSE labeling). (E) Protein level of the cytotoxic marker GZMB in CD8 + T cells detected by immunoblot analysis. (F and G) Proportions of IFN-γ + (F) and TNF-α + (G) CD8 + T cells analyzed by flow cytometry. (H and I) Proportions of TIM-3 + (E) and PD-1 + (F) CD8 + T cells analyzed by flow cytometry. (J) Viability of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined using CCK-8 assay. (K) Apoptosis of C666-1 cells after a 10-h direct co-culture with differentially treated CD8 + T cells determined by flow cytometry (FITC and PI labeling). Differences were compared by one-way (C–K) or two-way (A and B) ANOVA. ∗ vs. Normal medium; # vs. CM. ∗#p < 0.05. Three biological replicates were performed. The error bar denotes the standard deviation.
Article Snippet: The proportion of activated CD8 + T cells was screened by the Attune NxT flow cytometer (Thermo Fisher Scientific) using the antibodies of allophycocyanin (APC)-CD3 (ab239287, Abcam), fluorescein isothiocyanate (FITC)-CD8 (ab28010, Abcam),
Techniques: Western Blot, Flow Cytometry, Labeling, Marker, Co-Culture Assay, CCK-8 Assay, Standard Deviation
Journal: iScience
Article Title: EP300 restores the glycolytic activity and anti-tumor function of CD8 + cytotoxic T cells in nasopharyngeal carcinoma
doi: 10.1016/j.isci.2024.108957
Figure Lengend Snippet:
Article Snippet: The proportion of activated CD8 + T cells was screened by the Attune NxT flow cytometer (Thermo Fisher Scientific) using the antibodies of allophycocyanin (APC)-CD3 (ab239287, Abcam), fluorescein isothiocyanate (FITC)-CD8 (ab28010, Abcam),
Techniques: Recombinant, Activation Assay, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Luciferase, Reporter Assay, Sequencing, Software