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Image Search Results
Journal: Nucleic Acids Research
Article Title: Accelerated discovery and miniaturization of novel single-stranded cytidine deaminases
doi: 10.1093/nar/gkae800
Figure Lengend Snippet: In vivo mouse PCSK9 gene editing using single AAV8 - packaged SflSdd-CBE (nSaCas9) 2.0. ( A ) Theoretical packaging of SflSdd-CBE (nSaCas9) 2.0 into a single AAV8. The top, shows the architectures of AncBE4max (nSaCas9), BE4-gam (nSaCas9), and Sdd-CBE (nSaCas9) 2.0, with AncBE4max (nSaCas9) and BE4-gam (nSaCas9) in gray, indicating their size exceeds the single AAV packaging limit. The bottom presents a schematic representation of AAV8-SflSdd-CBE (nSaCas9) 2.0. ( B ) Experimental timeline for tail-vein injections and subsequent analyses using AAV8-SflSdd-CBE (nSaCas9) 2.0. ( C ) Editing efficiencies of SflSdd-CBE (nSaCas9) 2.0 at the Pcsk9 site in liver tissue post-injection of AAV8 at 5 × 10 11 total genome copies. (D-E) Four-week post-injection quantification of total serum cholesterol levels ( D ), serum ALB levels ( E ), serum ALT levels ( F ) and serum AST levels ( G ) (PBS, n = 5; AAV8- PCSK9 , n = 4). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Article Snippet: The
Techniques: In Vivo, Injection