Journal: Clinical and Translational Medicine

Article Title: Smad3‐mediated lncRNA HSALR1 enhances the non‐classic signalling pathway of TGF‐β1 in human bronchial fibroblasts by binding to HSP90AB1

doi: 10.1002/ctm2.1292

Figure Lengend Snippet: HSALR1 mice showed noticeable pathological differences of chronic obstructive pulmonary disease than wild‐type mice. (A) Schematic structures of AAV‐based negative control vector (AAV‐NC) and AAV‐based HSALR1 gene vector (AAV‐HSALR). (B) qRT‐PCR analysis of the expression of HSALR1 between AAV‐NC mice and AAV‐ HSALR1 mice (Number of mice in each group = 7, Student's t ‐test). (C) Workflow of AAV intratracheal instillation and cigarette smoke exposure. (D) Lung FEV20/FVC, RI, Cchord, Cydn and FRC were performed in 4 groups of mice. Cydn, lung dynamic compliance; Cchord, lung compliance; FRC, functional residual capacity; RI, respiratory index (Number of mice in each group = 7, Student's t ‐test). (E) Sections of the lung tissues from 4 groups mice were analysed by HE staining (Number of mice in each group = 7, Student's t ‐test). (F) Sections of the lung tissues from 4 groups mice were analysed by Masson staining (Number of mice in each group = 7, Student's t ‐test). (G) Sections of the lung tissues from 4 groups mice were analysed by IHC staining of α‐SMA (Number of mice in each group = 7, Student's t ‐test). (H) Sections of the lung tissues from 4 groups mice were analysed by IHC staining of FN1 (Number of mice in each group = 7, Student's t ‐test). Data information: Error bars represent means ± SD. * p < .05, ** p < .01 and *** p < .05.

Article Snippet: Adeno‐associated virus encoding HSALR1 (AAV‐ HSALR1 ) and AAV‐empty vector (AAV‐NC) were purchased from PackGene Biotech (Guangzhou, China).

Techniques: Negative Control, Plasmid Preparation, Quantitative RT-PCR, Expressing, Functional Assay, Staining, Immunohistochemistry