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Alomone Labs
ars α1d ![]() Ars α1d, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/aar-019/pmc04496866-59-64-72?v=Alomone+Labs Average 93 stars, based on 1 article reviews
ars α1d - by Bioz Stars,
2026-08
93/100 stars
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Journal: Arthritis Research & Therapy
Article Title: Reactivity of rat bone marrow-derived macrophages to neurotransmitter stimulation in the context of collagen II-induced arthritis
doi: 10.1186/s13075-015-0684-4
Figure Lengend Snippet: Neurotransmitter receptor profile during course of CIA. Adrenergic receptors alpha 1D, alpha 2B, and beta 2 and muscarinic ACh receptor M5 and the alternative VIP receptor PACAP receptor 1 were stained in BMMs from control and CIA animals 20 ( a ) and 40 ( b ) days post immunization. Paraformaldehyde-fixed cells were stained after 5 days of differentiation in the presence of M-CSF and RANKL, and sections containing only BMMs were photographed. Nuclei were counterstained with DAPI. N = 4 (control and CIA). Magnification 200×. ACh acetylcholine, AChR acetylcholine receptor, BMM bone marrow-derived macrophage, CIA collagen II-induced arthritis, DAPI 4′,6-diamidino-2-phenylindole, M-CSF macrophage colony-stimulating factor, NA noradrenaline, PACAP pituitary adenylate cyclase-activating peptide, RANKL receptor activator of nuclear factor-kappa-B ligand, VIP vasoactive intestinal peptide
Article Snippet: Neurotransmitter receptors were detected by using rabbit polyclonal immunoglobuline (Ig) G against rat pituitary adenylate cyclase-activating peptide (PACAP) receptor 1 (1:50, sc-30018, Santa Cruz Biotechnologies, Dallas, TX, USA) and rabbit polyclonal antibody against muscarinic ACh receptor M5 (1:400, ab41171) and rabbit polyclonal antibody against adrenoceptor (AR) β2 (3 μg/ml, ab36956; both Abcam, Cambridge, UK) as well as rabbit polyclonal antibodies against extracellular sections of
Techniques: Staining, Control, Derivative Assay