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Revvity
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Image Search Results
Journal: Journal of Cancer
Article Title: In vivo studies of a peptidomimetic that targets EGFR dimerization in NSCLC
doi: 10.7150/jca.46320
Figure Lengend Snippet: Effect of 18 on phosphorylation of HER2 and Akt. A549 lung cancer cell lines were incubated with 18 and after 36 h, cells were washed and lysed. Western blot was performed to determine p-HER2 and p-Akt as well as total HER2 and Akt. (Blots were cropped and presented). Quantification of signal intensities were done by ImageJ). Lapatinib was used as positive control. A) Western blot of HER2, p-HER2, Akt, p-Akt. Equal loading of GAPDH was used for comparison. Experiments were repeated three times. Quantification of B) p-HER2 and C) p-Akt using densitometry. Band intensity was represented with scaling from GAPDH band intensity. * p< 0.05, ** p< 0.01.
Article Snippet:
Techniques: Phospho-proteomics, Incubation, Western Blot, Positive Control, Comparison
Journal: Journal of Cancer
Article Title: In vivo studies of a peptidomimetic that targets EGFR dimerization in NSCLC
doi: 10.7150/jca.46320
Figure Lengend Snippet: Therapeutic effect of 18 in NSCLC mice model. NSCLC tumors were induced in mice using luciferase transfected A549 cells. Mice were imaged using an in vivo imaging instrument after injection of luciferin for tumor growth was monitored. Compound 18 was injected via tail vein, and lapatinib (via intraperitoneal injection) was used as a positive control. A) Representative images of tumor growth in mice. Image was processed using software, and ROIs were drawn on the luciferase intensity to quantify the intensity. B) Plot of log transformed total flux representing the tumor volume vs. time in days using box and whisker plot. The total flux (p/s) values were log transformed to meet assumptions of an ANOVA. Compound 18 inhibits the tumor growth in lungs significantly compared to control.
Article Snippet:
Techniques: Luciferase, Transfection, In Vivo Imaging, Injection, Positive Control, Software, Transformation Assay, Whisker Assay, Control