Journal:

Article Title: PP2B-mediated Dephosphorylation of c-Jun C Terminus Regulates Phorbol Ester-induced c-Jun/Sp1 Interaction in A431 Cells

doi: 10.1091/mbc.E06-09-0797

Figure Lengend Snippet: Effect of PP2B on PMA-induced genes expression. (A and B) Cells were transfected with pXLO-7-1, SPM7, pCMVβ-galactosidase, or various amounts of PP2B expression vector by lipofection. Cells were treated with 5 nM PMA and further cultured in fresh medium up to 18 h. The luciferase and β-galactosidase activities were then determined. Values represent means ± SEM of three determinations. (C) Cells were transfected with 50 nM PP2B siRNA or SC oligonucleotides by lipofection. After PMA treatment for 18 h, total RNA was extracted for reverse transcription PCR with 12(S)-lipoxygenase and glyceraldehyde-3-phosphate dehydrogenase primers. Expressions of PP2B and Sp1 proteins were analyzed by Western blot analysis by using anti-PP2B and Sp1 antibodies, respectively. Similar results were obtained in two independent experiments. (D) A549 cells were transfected with pPLA 599, pCMVβ-galactosidase, 50 nM PP2B siRNA, or SC oligonucleotides by lipofection. After PMA treatment for 16 h, the luciferase and β-galactosidase activities were then determined. Values represent means ± SEM of three determinations.

Article Snippet: Cell Culture Human epidermoid carcinoma A431 and nonsmall-cell lung carcinoma (NSCLC) A549 cells were grown at 37°C under 5% CO 2 in 10-cm plastic dishes containing 10 ml of DMEM (Invitrogen, Carlsbad, CA) and Nutrition mixture F12 Kaighn's modification medium (Biowest, Miami, FL), respectively and supplemented with 10% fetal bovine serum, 100 μg/ml streptomycin, and 100 U/ml penicillin.

Techniques: Expressing, Transfection, Plasmid Preparation, Cell Culture, Luciferase, Western Blot

Journal: Journal of Ginseng Research

Article Title: Ginsenoside Rg5 overcomes chemotherapeutic multidrug resistance mediated by ABCB1 transporter: in vitro and in vivo study

doi: 10.1016/j.jgr.2018.10.007

Figure Lengend Snippet: Chemical structure and cytotoxicity of Rg5 in MDR cancer cells. (A) Chemical structure of Rg5. (B) Cytotoxicity of Rg5 alone in pairs of A2780/T or A2780 cells. (C) The cells were treated with various concentrations of docetaxel (TXT) for 48 hours in pairs of A2780/T or A2780 cells. (D) Cytotoxicity of Rg5 alone in pairs of A549/T or A549 cells. (E) The cells were treated with various concentrations of TXT for 48 hours in pairs of A549/T or A549 cells. Cell growth was determined using the SRB assay (n ≥ 3). MDR, multidrug resistance; SRB, Sulforhodamine B.

Article Snippet: Extracellular signal–regulated kinases (ERK) 1/2 and actin antibodies were purchased from Santa Cruz Biotechnology; ABCB1 (P-gp) antibodies were purchased from Calbiochem, USA; Nrf2 antibodies were purchased from Abcam, Hong Kong; other antibodies such as AKT, P-AKT, and P-ERK1/2 were purchased from Cell Signaling Technology, Inc. Human ovarian cancer cells A2780, human nonsmall cell lung cancer (NSCLC) A549 and their PTX-resistant cell line A2780/T, and A549/T were purchased from KeyGen Biotech Co., Ltd, Nanjing, China.

Techniques: Sulforhodamine B Assay

Journal: Journal of Ginseng Research

Article Title: Ginsenoside Rg5 overcomes chemotherapeutic multidrug resistance mediated by ABCB1 transporter: in vitro and in vivo study

doi: 10.1016/j.jgr.2018.10.007

Figure Lengend Snippet: Rg5 recovered sensitivity to docetaxel. Cells were treated with the indicated drugs for 48 hours and subjected to SRB assay. Rg5 reduces the IC 50 of TXT in resistant cancer cells (A2780/T) (B) and A549/T (D) but not in drug sensitive (A2780) (A) and A549 (C) . (E) Rg5 inhibited the colony formation of TXT in resistant cancer cells A2780/T in a dose-dependent manner. ## ,** p < 0.01, ### ,*** p < 0.001 vs absence of Rg5. TXT, docetaxel; SRB, Sulforhodamine B.

Article Snippet: Extracellular signal–regulated kinases (ERK) 1/2 and actin antibodies were purchased from Santa Cruz Biotechnology; ABCB1 (P-gp) antibodies were purchased from Calbiochem, USA; Nrf2 antibodies were purchased from Abcam, Hong Kong; other antibodies such as AKT, P-AKT, and P-ERK1/2 were purchased from Cell Signaling Technology, Inc. Human ovarian cancer cells A2780, human nonsmall cell lung cancer (NSCLC) A549 and their PTX-resistant cell line A2780/T, and A549/T were purchased from KeyGen Biotech Co., Ltd, Nanjing, China.

Techniques: Sulforhodamine B Assay

Journal: Journal of Ginseng Research

Article Title: Ginsenoside Rg5 overcomes chemotherapeutic multidrug resistance mediated by ABCB1 transporter: in vitro and in vivo study

doi: 10.1016/j.jgr.2018.10.007

Figure Lengend Snippet: Rg5 sensitized the chemotherapy Drug Paclitaxel, docetaxel, 5-fluorouracil, daunorubicin, and doxorubicin to ABCB1-Mediated Drug resistance A2780/T Cells.

Article Snippet: Extracellular signal–regulated kinases (ERK) 1/2 and actin antibodies were purchased from Santa Cruz Biotechnology; ABCB1 (P-gp) antibodies were purchased from Calbiochem, USA; Nrf2 antibodies were purchased from Abcam, Hong Kong; other antibodies such as AKT, P-AKT, and P-ERK1/2 were purchased from Cell Signaling Technology, Inc. Human ovarian cancer cells A2780, human nonsmall cell lung cancer (NSCLC) A549 and their PTX-resistant cell line A2780/T, and A549/T were purchased from KeyGen Biotech Co., Ltd, Nanjing, China.

Techniques:

Journal: Journal of Ginseng Research

Article Title: Ginsenoside Rg5 overcomes chemotherapeutic multidrug resistance mediated by ABCB1 transporter: in vitro and in vivo study

doi: 10.1016/j.jgr.2018.10.007

Figure Lengend Snippet: Effects of treatment of Rg5 and docetaxel on A549/T cell xenograft nude mice model. (A) The body weight was drawn to monitor the body weight and tumor volume with time after implantation . (B) Tumour growth curves were drawn to monitor the body weight and tumor volume with time after implantation. The tumor xenografts were excised. (C) The tumors were photographed on the 27th day after implantation. (D) The tumors weighted on the 27th day after implantation. The data shown are expressed as the mean ± SD for each group (n = 9 or 10), * p < 0.05, ** p < 0.01, ** p < 0.001 . CTR, control group given vehicle; SD, standard deviation; TXT, docetaxel.

Article Snippet: Extracellular signal–regulated kinases (ERK) 1/2 and actin antibodies were purchased from Santa Cruz Biotechnology; ABCB1 (P-gp) antibodies were purchased from Calbiochem, USA; Nrf2 antibodies were purchased from Abcam, Hong Kong; other antibodies such as AKT, P-AKT, and P-ERK1/2 were purchased from Cell Signaling Technology, Inc. Human ovarian cancer cells A2780, human nonsmall cell lung cancer (NSCLC) A549 and their PTX-resistant cell line A2780/T, and A549/T were purchased from KeyGen Biotech Co., Ltd, Nanjing, China.

Techniques: Standard Deviation

Journal: Journal of Ginseng Research

Article Title: Ginsenoside Rg5 overcomes chemotherapeutic multidrug resistance mediated by ABCB1 transporter: in vitro and in vivo study

doi: 10.1016/j.jgr.2018.10.007

Figure Lengend Snippet: Chemical structure and cytotoxicity of Rg5 in MDR cancer cells. (A) Chemical structure of Rg5. (B) Cytotoxicity of Rg5 alone in pairs of A2780/T or A2780 cells. (C) The cells were treated with various concentrations of docetaxel (TXT) for 48 hours in pairs of A2780/T or A2780 cells. (D) Cytotoxicity of Rg5 alone in pairs of A549/T or A549 cells. (E) The cells were treated with various concentrations of TXT for 48 hours in pairs of A549/T or A549 cells. Cell growth was determined using the SRB assay (n ≥ 3). MDR, multidrug resistance; SRB, Sulforhodamine B.

Article Snippet: Extracellular signal–regulated kinases (ERK) 1/2 and actin antibodies were purchased from Santa Cruz Biotechnology; ABCB1 (P-gp) antibodies were purchased from Calbiochem, USA; Nrf2 antibodies were purchased from Abcam, Hong Kong; other antibodies such as AKT, P-AKT, and P-ERK1/2 were purchased from Cell Signaling Technology, Inc. Human ovarian cancer cells A2780, human nonsmall cell lung cancer (NSCLC) A549 and their PTX-resistant cell line A2780/T, and A549/T were purchased from KeyGen Biotech Co., Ltd, Nanjing, China.

Techniques: Sulforhodamine B Assay

Journal: Journal of Ginseng Research

Article Title: Ginsenoside Rg5 overcomes chemotherapeutic multidrug resistance mediated by ABCB1 transporter: in vitro and in vivo study

doi: 10.1016/j.jgr.2018.10.007

Figure Lengend Snippet: Rg5 recovered sensitivity to docetaxel. Cells were treated with the indicated drugs for 48 hours and subjected to SRB assay. Rg5 reduces the IC 50 of TXT in resistant cancer cells (A2780/T) (B) and A549/T (D) but not in drug sensitive (A2780) (A) and A549 (C) . (E) Rg5 inhibited the colony formation of TXT in resistant cancer cells A2780/T in a dose-dependent manner. ## ,** p < 0.01, ### ,*** p < 0.001 vs absence of Rg5. TXT, docetaxel; SRB, Sulforhodamine B.

Article Snippet: Extracellular signal–regulated kinases (ERK) 1/2 and actin antibodies were purchased from Santa Cruz Biotechnology; ABCB1 (P-gp) antibodies were purchased from Calbiochem, USA; Nrf2 antibodies were purchased from Abcam, Hong Kong; other antibodies such as AKT, P-AKT, and P-ERK1/2 were purchased from Cell Signaling Technology, Inc. Human ovarian cancer cells A2780, human nonsmall cell lung cancer (NSCLC) A549 and their PTX-resistant cell line A2780/T, and A549/T were purchased from KeyGen Biotech Co., Ltd, Nanjing, China.

Techniques: Sulforhodamine B Assay

Journal: Journal of Ginseng Research

Article Title: Ginsenoside Rg5 overcomes chemotherapeutic multidrug resistance mediated by ABCB1 transporter: in vitro and in vivo study

doi: 10.1016/j.jgr.2018.10.007

Figure Lengend Snippet: Rg5 sensitized the chemotherapy Drug Paclitaxel, docetaxel, 5-fluorouracil, daunorubicin, and doxorubicin to ABCB1-Mediated Drug resistance A2780/T Cells.

Article Snippet: Extracellular signal–regulated kinases (ERK) 1/2 and actin antibodies were purchased from Santa Cruz Biotechnology; ABCB1 (P-gp) antibodies were purchased from Calbiochem, USA; Nrf2 antibodies were purchased from Abcam, Hong Kong; other antibodies such as AKT, P-AKT, and P-ERK1/2 were purchased from Cell Signaling Technology, Inc. Human ovarian cancer cells A2780, human nonsmall cell lung cancer (NSCLC) A549 and their PTX-resistant cell line A2780/T, and A549/T were purchased from KeyGen Biotech Co., Ltd, Nanjing, China.

Techniques:

Journal: Journal of Ginseng Research

Article Title: Ginsenoside Rg5 overcomes chemotherapeutic multidrug resistance mediated by ABCB1 transporter: in vitro and in vivo study

doi: 10.1016/j.jgr.2018.10.007

Figure Lengend Snippet: Effects of treatment of Rg5 and docetaxel on A549/T cell xenograft nude mice model. (A) The body weight was drawn to monitor the body weight and tumor volume with time after implantation . (B) Tumour growth curves were drawn to monitor the body weight and tumor volume with time after implantation. The tumor xenografts were excised. (C) The tumors were photographed on the 27th day after implantation. (D) The tumors weighted on the 27th day after implantation. The data shown are expressed as the mean ± SD for each group (n = 9 or 10), * p < 0.05, ** p < 0.01, ** p < 0.001 . CTR, control group given vehicle; SD, standard deviation; TXT, docetaxel.

Article Snippet: Extracellular signal–regulated kinases (ERK) 1/2 and actin antibodies were purchased from Santa Cruz Biotechnology; ABCB1 (P-gp) antibodies were purchased from Calbiochem, USA; Nrf2 antibodies were purchased from Abcam, Hong Kong; other antibodies such as AKT, P-AKT, and P-ERK1/2 were purchased from Cell Signaling Technology, Inc. Human ovarian cancer cells A2780, human nonsmall cell lung cancer (NSCLC) A549 and their PTX-resistant cell line A2780/T, and A549/T were purchased from KeyGen Biotech Co., Ltd, Nanjing, China.

Techniques: Standard Deviation